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Träfflista för sökning "WFRF:(Collins A) srt2:(1995-1999);pers:(Collins R)"

Sökning: WFRF:(Collins A) > (1995-1999) > Collins R

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  • Collins, A R, et al. (författare)
  • Cystatin D, a natural salivary cysteine protease inhibitor, inhibits coronavirus replication at its physiologic concentration
  • 1998
  • Ingår i: Oral Microbiology and Immunology. - : Wiley. - 0902-0055 .- 1399-302X. ; 13:1, s. 59-61
  • Tidskriftsartikel (refereegranskat)abstract
    • This study was conducted to examine the effect of cystatin D, a newly discovered salivary cysteine protease inhibitor, on human coronavirus replication. When MRC-5, human diploid lung cells, were incubated with dilutions of recombinant human cystatin D from 0.65-10 microM for 1 h prior to, during and after infection with coronavirus OC43 and 229e strains, a decrease in virus yield was observed resulting in an IC50 of 0.8 microM for both virus strains. This dose is within the normal concentration range of cystatin D, 0.12-1.9 microM found in saliva. When a single dose, 2.5 microM, was applied, cystatin inhibition of release of virus progeny was not overcome until three days post infection whereas inhibition by leupeptin, a serine and cysteine protease inhibitor, was completely abrogated by two days. When cellular toxicity was measured by 3H-thymidine uptake, cystatin D did not markedly affect cell proliferation below a 10 microM dose. The results demonstrate that cystatin D is a potent inhibitor of coronavirus replication.
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