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Putative Epigenetic...
Putative Epigenetic Biomarkers of Stress in Red Blood Cells of Chickens Reared Across Different Biomes
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- Pértille, Fábio (författare)
- Linköpings universitet,Biologi,Tekniska fakulteten,Univ Sao Paulo, Brazil
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- Ibelli, Adriana Mercia Guaratini (författare)
- Embrapa Suinos & Aves, Concordia, Brazil.
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- El Sharif, Maj (författare)
- Linköpings universitet,Biologi,Tekniska fakulteten
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- Poleti, Mirele Daiana (författare)
- Univ Sao Paulo, Fac Anim Sci & Food Engn FZEA, Anim Sci Program, Pirassununga, Brazil.
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- Frohlich, Anna Sophie (författare)
- Linköpings universitet,Biologi,Tekniska fakulteten
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- Rezaei, Shiva (författare)
- Linköpings universitet,Biologi,Tekniska fakulteten,Avian Behavioural Genomics and Physiology Group
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- Ledur, Monica Correa (författare)
- Embrapa Suinos & Aves, Concordia, Brazil.
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- Jensen, Per (författare)
- Linköpings universitet,Biologi,Tekniska fakulteten
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- Guerrero-Bosagna, Carlos (författare)
- Linköpings universitet,Uppsala universitet,Institutionen för organismbiologi,Linkoping Univ, IFM Biol, Avian Behav Genom & Physiol Grp, Linkoping, Sweden.,Biologi,Tekniska fakulteten,Uppsala Univ, Sweden
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- Coutinho, Luiz Lehmann (författare)
- Univ Sao Paulo, Luiz de Queiroz Coll Agr ESALQ, Anim Sci & Pastures Dept, Anim Biotechnol Lab, Piracicaba, Brazil.
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(creator_code:org_t)
- 2020-11-02
- 2020
- Engelska.
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Ingår i: Frontiers in Genetics. - : Frontiers Media SA. - 1664-8021. ; 11
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Abstract
Ämnesord
Stäng
- Production animals are constantly subjected to early adverse environmental conditionsthat influence the adult phenotype and produce epigenetic effects. CpG dinucleotidemethylation in red blood cells (RBC) could be a useful epigenetic biomarker to identifyanimals subjected to chronic stress in the production environment. Here we compareda reduced fraction of the RBC methylome of chickens exposed to social isolation tonon-exposed. These experiments were performed in two different locations: Brazil andSweden. The aim was to identify stress-associated DNA methylation profiles in RBC across these populations, in spite of the variable conditions to which birds are exposedin each facility and their different lineages. Birds were increasingly exposed to a socialisolation treatment, combined with food and water deprivation, at random periods of theday from weeks 1–4 after hatching. We then collected the RBC DNA from individualsand compared a reduced fraction of their methylome between the experimental groupsusing two bioinformatic approaches to identify differentially methylated regions (DMRs):one using fixed-size windows and another that preselected differential peaks with MACS2. Three levels of significance were used (P ≤ 0.05, P ≤ 0.005, and P ≤ 0.0005) to identify DMRs between experimental groups, which were then used for differentanalyses. With both of the approaches more DMRs reached the defined significancethresholds in BR individuals compared to SW. However, more DMRs had higher foldchange values in SW compared to BR individuals. Interestingly, ChrZ was enrichedabove expectancy for the presence of DMRs. Additionally, when analyzing the locationsof these DMRs in relation to the transcription starting site (TSS), we found threepeaks with high DMR presence: 10 kb upstream, the TSS itself, and 20–40 kbdownstream. Interestingly, these peaks had DMRs with a high presence (>50%) ofspecific transcription factor binding sites. Three overlapping DMRs were found betweenthe BR and SW population using the most relaxed p-value (P ≤ 0.05). With the most stringent p-value (P ≤ 0.0005), we found 7 and 4 DMRs between treatmentsin the BR and SW populations, respectively. This study is the first approximation toidentify epigenetic biomarkers of long-term exposure to stress in different lineages ofproduction animals.
Ämnesord
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Evolutionsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Evolutionary Biology (hsv//eng)
Nyckelord
- stress
- red blood cells
- epigenetics
- chicken
- DNA methylation
- animal welfare
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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