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Olfactory Receptor 51E1 as a Novel Target for Diagnosis in Somatostatin Receptor Negative Lung Carcinoids

Giandomenico, Valeria (författare)
Uppsala universitet,Onkologisk endokrinologi,Science for Life Laboratory, SciLifeLab
Cui, Tao (författare)
Uppsala universitet,Onkologisk endokrinologi
Grimelius, Lars (författare)
Uppsala universitet,Molekylär och morfologisk patologi
visa fler...
Öberg, Kjell (författare)
Uppsala universitet,Onkologisk endokrinologi,Science for Life Laboratory, SciLifeLab
Pelosi, Giuseppe (författare)
European Institute of Oncology, Milan, Italy; University of Milan School of Medicine, Milan, Italy,Division of Pathogy and Laboratory Medicine; Department of Biochemical and Clinical Sciences "Luigi Sacco"
Tsolakis, Apostolos V. (författare)
Uppsala universitet,Onkologisk endokrinologi
visa färre...
 (creator_code:org_t)
2013
2013
Engelska.
Ingår i: Journal of Molecular Endocrinology. - 0952-5041 .- 1479-6813. ; 51, s. 277-286
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • Somatostatin receptors (SSTRs) may be used in lung carcinoids (LCs) for diagnosis and therapy, although additional targets are clearly warranted. This study aimed to investigate whether olfactory receptor 51E1 (OR51E1) may be a potential target for LCs. OR51E1 coding sequence was analyzed in LC cell lines, NCI-H727 and NCI-H720. OR51E1 transcript expression was investigated in LC cell lines and frozen specimens by quantitative real-time PCR. OR51E1, SSTR2, SSTR3, and SSTR5 expression was evaluated by immunohistochemistry on paraffin-embedded sections of 73 typical carcinoids (TCs), 14 atypical carcinoids (ACs) and 11 regional/distant metastases, and compared to OctreoScan data. Immunohistochemistry results were rendered semiquantitatively on a scale from 0 to 3+, taking into account the cellular compartmentalization (membrane vs. cytoplasm) and the percentage of tumor cells (<50% vs. >50%). Our results showed that wild-type OR51E1 transcript was expressed in both LC cell lines. OR51E1 mRNA was expressed in 9/12 TCs and 7/9 ACs (p=NS). Immunohistochemically, OR51E1, SSTR2, SSTR3 and SSTR5 were detected in 85%, 71%, 25% and 39% of TCs, and in 86%, 79%, 43% and 36% of ACs, respectively. OR51E1 immunohistochemical scores were higher or equal compared to SSTRs in 79% of TCs and 86% of ACs. Furthermore, in the LC cases where all SSTR subtypes were lacking, membrane OR51E1 expression was detected in 10/17 TCs and 1/2 ACs. Moreover, higher OR51E1 immunohistochemical scores were detected in 5/6 OctreoScan-negative LC lesions. Therefore, the high expression of OR51E1 in LCs makes it a potential novel diagnostic target in SSTR-negative tumors.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)

Nyckelord

Olfactory receptor 51E1
lung carcinoids
novel target for diagnosis
somatostatin receptors
OctreoScan
Endokrinologi och Diabetologi
Endocrinology and Diabetology
Lung Medicine
Lungmedicin
Molecular Biology
Molekylärbiologi
Oncology
Onkologi

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