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- Glasbey, JC, et al.
(författare)
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- 2021
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swepub:Mat__t
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| 4. |
- Cossarizza, A., et al.
(författare)
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
- 2019
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Ingår i: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 49:10, s. 1457-1973
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Tidskriftsartikel (refereegranskat)abstract
- These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
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| 5. |
- Kuschmierz, Paul, et al.
(författare)
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European first-year university students accept evolution but lack substantial knowledge about it : A standardized European cross-country assessment
- 2021
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Ingår i: Evolution. - : BioMed Central (BMC). - 1936-6426 .- 1936-6434. ; 14:1, s. 1-22
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Tidskriftsartikel (refereegranskat)abstract
- Background: Investigations of evolution knowledge and acceptance and their relation are central to evolution education research. Ambiguous results in this field of study demonstrate a variety of measuring issues, for instance differently theorized constructs, or a lack of standardized methods, especially for cross-country comparisons. In particular, meaningful comparisons across European countries, with their varying cultural backgrounds and education systems, are rare, often include only few countries, and lack standardization. To address these deficits, we conducted a standardized European survey, on 9200 first-year university students in 26 European countries utilizing a validated, comprehensive questionnaire, the “Evolution Education Questionnaire”, to assess evolution acceptance and knowledge, as well as influencing factors on evolution acceptance. Results: We found that, despite European countries’ different cultural backgrounds and education systems, European first-year university students generally accept evolution. At the same time, they lack substantial knowledge about it, even if they are enrolled in a biology-related study program. Additionally, we developed a multilevel-model that determines religious faith as the main influencing factor in accepting evolution. According to our model, knowledge about evolution and interest in biological topics also increase acceptance of evolution, but to a much lesser extent than religious faith. The effect of age and sex, as well as the country’s affiliation, students’ denomination, and whether or not a student is enrolled in a biology-related university program, is negligible. Conclusions: Our findings indicate that, despite all their differences, most of the European education systems for upper secondary education lead to acceptance of evolution at least in university students. It appears that, at least in this sample, the differences in knowledge between countries reflect neither the extent to which school curricula cover evolutionary biology nor the percentage of biology-related students in the country samples. Future studies should investigate the role of different European school curricula, identify particularly problematic or underrepresented evolutionary concepts in biology education, and analyze the role of religious faith when teaching evolution.
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| 6. |
- Cvetkovic, Vladimir, et al.
(författare)
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Parameter and model sensitivities for colloid-facilitated radionuclide transport on the field scale
- 2004
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Ingår i: Water resources research. - : American Geophysical Union (AGU). - 0043-1397 .- 1944-7973. ; 40:6
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Tidskriftsartikel (refereegranskat)abstract
- We investigate the potential effects of inorganic colloids on radionuclide transport in groundwater using generic sensitivity studies and an example based on the alluvial aquifer near Yucca Mountain, Nevada. Our emphasis is on kinetically controlled sorption of radionuclides on mobile and immobile colloids. Three kinetic sorption models are considered for the sensitivity analysis: bilinear, Langmuir, and linear. Plutonium is assumed to be injected into the Yucca Mountain alluvial aquifer at a constant rate and follows a random stream tube to a monitoring boundary. The linear sorption model provides a reasonable upper bound on colloid-facilitated plutonium transport for the site-specific conditions. In the absence of colloid filtration and retardation, colloids enhance the plutonium discharge by a large factor over the situation without colloids. Exchange of plutonium between solution and reversibly attached colloids makes colloid retardation relatively ineffective at reducing colloid-facilitated transport except when the retardation factor is large. Irreversible removal of colloids (filtration) is more effective than retardation at reducing colloid-facilitated transport. For fixed filtration rate the degree of attenuation depends sensitively and nonmonotonically on the rate of plutonium desorption from colloids. These results emphasize the need for accurate measurements of rates of desorption from colloids as well as in situ studies of filtration of naturally occurring colloids.
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| 7. |
- Painter, S., et al.
(författare)
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Significance of kinetics for sorption on inorganic colloids : Modeling and experiment interpretation issues
- 2002
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Ingår i: Environmental Science and Technology. - : American Chemical Society (ACS). - 0013-936X .- 1520-5851. ; 36:24, s. 5369-5375
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Tidskriftsartikel (refereegranskat)abstract
- A two-site kinetic model for solute sorption on inorganic colloids is developed. The model quantifies linear first-order sorption on two types of sites (fast and slow) characterized by two pairs of rates (forward and reverse). We use the model to explore data requirements for long-term predictive calculations of colloid-facilitated transport and to evaluate laboratory kinetic sorption data of Lu et al.. Five batch sorption data sets are considered with plutonium as the tracer and montmorillonite, hematite, silica, and smectite as colloids. Using asymptotic results applicable on the time stale of limited duration experiments, a robust estimation procedure is developed for the fast-site partitioning coefficient K-c and the slow forward rate alpha. The estimated range of K-c is 1.1-76 L/g, and the range for a is 0.0017-0.02 1/h. The fast reverse rate k(r) is estimated in the range 0.012-0.1 1/h. Comparison of one-site and two-site sorption interpretations reveals the difficulty in, discriminating between the two models for montmorillonite and to a lesser extent for hematite. For silica and smectite, the two-site model clearly provides a better representation of the data as compared with a single site model. Kinetic data for silica are available for different colloid concentrations (0.2 g/L and 1 g/L). For the range of experimental conditions considered, a. appears to be independent of colloid concentration.
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