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Sökning: WFRF:(Daniel Maria) > Doktorsavhandling

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1.
  • Wu, Jiechen (författare)
  • Systems Perspectives on Modelling and Managing Future Anthropogenic Emissions in Urban Areas : Nitrogen, Phosphorus and Carbon Studies in Stockholm, Sweden
  • 2016
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • Managing anthropogenic emissions in urban areas is a major challenge in sustainable environmental development for cities, and future changes and increasing urbanisation may increase this challenge. Systems perspectives have become increasingly important in helping urban managers understand how different changes may alter future emissions and whether current management strategies can efficiently manage these emissions. This thesis provides some systems perspectives that have been lacking in previous studies on modelling and managing future anthropogenic emissions in urban areas. The city of Stockholm, Sweden, was selected as the study site and studies about nitrogen, phosphorus and carbon were chosen, given world-wide urban eutrophication and global concerns about climate change. A substance flow analysis (SFA) structured model, comprising a source model coupled with a watershed model in an SFA structure, was developed to investigate future nutrient loading scenarios under various urban changes in small urban lake catchments. The results demonstrated that climate change potentially posed a greater threat to future nutrient loads to a selected lake catchment in Stockholm than the other scenarios examined. Another SFA-based study on future phosphorus flows through the city of Stockholm indicated that the best management option may depend on the perspective applied when comparing future scenarios of phosphorus flows and that both upstream and downstream measures need to be considered in managing urban phosphorus flows. An evaluation approach for examining current management plans and low-carbon city initiatives using the Driving forces-Pressure-States-Impact-Response (DPSIR) framework, was formulated. With such an evaluation approach, investigation of how well selected plans cover different aspects of the DPSIR framework and whether root causes and systematic measures are highlighted is possible. The results revealed that the current low-carbon city initiative in Stockholm falls within pressure-based, driver-orientated plans and that technical, institutional and cognitional measures are generally well covered. 
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2.
  • Bladh, Daniel, 1980- (författare)
  • Didaktiska val i partidemokratin : Om partiskolning och lärande i politiken
  • 2023
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • Syftet med avhandlingen är att undersöka de överväganden och skäl som ligger bakom didaktiska val som fattas när utbildning organiseras för partipolitiskt aktiva personer. De anordnare som främst står i fokus för avhandlingen är de nationella partiorganisationerna medan den kommunala förvaltningen fungerar som en kon-trastpunkt. Avhandlingens teoretiska perspektiv utgörs av vuxendidaktik samt av ett analytiskt schema där politiska partier förstås som strategiska aktörer. Det didaktiska kunskapsintresset riktar sig främst mot det politiska systemet, partierna som aktörer samt de egna partimedlemmarna. Det är kvalitativ forskning som ligger till grund för de specifika studier som avhandlingen omfattar. Det empiriska materialet har genererats genom kvalitativa forskningsintervjuer, insamling av olika typer av dokument samt omfattande kartläggning.Avhandlingen utgör en sammanläggningsavhandling som omfattar fyra artiklar. Artikel I analyserar organisering av partiskolning som verktyg för nationella partiorganisationer. Artikel II lyfter fram fostransprocesser som de nationella partiorganisationerna söker främja genom organisering av partiskolning riktat till partimedlemmar och lokalföreningar. Artikel III lyfter blicken gentemot det politiska systemet och visar hur partiskolning både kan påverka och påverkas av hur olika partifunktioner utövas. Artikel IV tar sikte på den utbildningsverksamhet som genomförs i svenska kommuner och lyfter fram tre tankelinjer som uttryck för hur de kommunala anordnarna resonerar kring och motiverar den utbildning som de erbjuder till lokala folkvalda.  Sammantaget bidrar avhandlingen med kunskap om vad som sker i mötet mellan utbildning och partidemokratin. Påverkan och fostran lyfts fram som två centrala skäl bakom de didaktiska val som fattas av de nationella partiorganisationerna vid organisering av partiskolning. Hur de nationella partiorganisationerna motiverar och resonerar kring den utbildningsverksamhet som de organiserar diskuteras i samband med samlingsbegreppen personer, process och platsen. Avhandlingen visar också hur partiskolning innehar en strategisk potential för partierna. Organisering av utbildningsverksamhet i politiska partier och kommuner kan även påverka konstruktionen av deltagarnas demokratiska kompetens, vilket kan spela roll för hur partimedlemmarnas aktiva medborgarskap kommer till uttryck.
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3.
  • Carlsson, Daniel O (författare)
  • Structural and Electrochemical Properties of Functionalized Nanocellulose Materials and Their Biocompatibility
  • 2014
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • Nanocellulose has received considerable interest during the last decade because it is renewable and biodegradable, and has excellent mechanical properties, nanoscale dimensions and wide functionalization possibilities. It is considered to be a unique and versatile platform on which new functional materials can be based.This thesis focuses on nanocellulose from wood (NFC) and from Cladophora algae (CNC), functionalized with surface charges or coated with the conducting polymer polypyrrole (PPy), aiming to study the influence of synthesis processes on structural and electrochemical properties of such materials and assess their biocompatibility.The most important results of the work demonstrated that 1) CNC was oxidized to the same extent using electrochemical TEMPO-mediated oxidation as with conventional TEMPO processes, which may facilitate easier reuse of the reaction medium; 2) NFC and CNC films with or without surface charges were non-cytotoxic as assessed by indirect in vitro testing. Anionic TEMPO-CNC films promoted fibroblast adhesion and proliferation in direct in vitro cytocompatibility testing, possibly due to its aligned fibril structure; 3) Rinsing of PPy-coated nanocellulose fibrils, which after drying into free-standing porous composites are applicable for energy storage and electrochemically controlled ion extraction, significantly degraded the PPy coating, unless acidic rinsing was employed. Only minor degradation was observed during long-term ambient storage; 4) Variations in the drying method as well as type and amount of nanocellulose offered ways of tailoring the porosities of nanocellulose/PPy composites between 30% and 98%, with increments of ~10%. Supercritical CO2-drying generated composites with the largest specific surface area yet reported for nanocellulose/conducting polymer composites (246 m2/g). The electrochemical oxidation rate was found to be controlled by the composite porosity; 5) In blood compatibility assessments for potential hemodialysis applications, heparinization of CNC/PPy composites was required to obtain thrombogenic properties comparable to commercial hemodialysis membranes. The pro-inflammatory characteristics of non-heparinized and heparinized composites were, to some extent, superior to commercial membranes. The heparin coating did not affect the solute extraction capacity of the composite.The presented results are deemed to be useful for tuning the properties of systems based on the studied materials in e.g. energy storage, ion exchange and biomaterial applications.
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4.
  • Daniel, Maria (författare)
  • Diagnosis, risk factors and quality-of-life in patients with myocardial infarction and normal coronary arteries
  • 2018
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • Background: Myocardial infarction with normal coronary arteries (MINCA) is a common condition that mostly affects middle-aged women. The pathogenesis is complex and includes various mechanisms that need to be explored for appropriate diagnosis and treatment. Patients with MINCA often complain about low energy and appear to be distressed. Previous studies in MINCA with control groups were lacking when the Stockholm myocardial infarction and normal coronaries (SMINC) study started. Aims: To describe cardiac magnetic resonance (CMR) imaging findings, background characteristics, atherosclerosis markers and quality-of-life (QoL) in patients with MINCA. The intention is to better understand and improve the management of this group of patients. Specific aims, methods and results: Study I: The aim was to report the true prevalence of myocarditis and MINCA with or without myocardial infarction by using CMR. We investigated 152 patients 35-70 years and found that 67% had a normal CMR, 19% had signs of myocardial necrosis and 7% had signs of myocarditis. Twenty-two percent of all MINCA with a normal CMR fulfilled the Mayo clinical diagnostic criteria for TS. The CMR was performed a median of 12 days (6-28 days) after the acute event. Study 2: The aim was to describe the risk factors by analysing the case record form (CRF) and different investigations performed during the 3 months follow up after the acute event in patients with MINCA and compare those with two control groups. We analysed blood samples, reactive hyperaemia index (RHI) and intima-media thickness (IMT) by using EndoPAT® (Itamar Medical Ltd) and ultrasound of the carotids. The results showed that MINCA was associated with similar risk factors as in coronary heart disease (CHD) patients except for a more favourable lipid profile. The atherosclerotic burden in MINCA, measured as RHI and IMT, were within the normal range and similar to both healthy and CHD controls. Psychiatric disorders were more common in patients with MINCA and TS than those without TS and more than half of all MINCA patients recalled physical and emotional stress before admission. Study 3: The aim was to describe the physical capacity and QoL 6 weeks to 3 months after the acute event in MINCA compared to both control groups using an exercise bicycle stress test and Short Form (SF)-36. The findings showed that patients with MINCA had a lower exercise capacity and QoL compared with healthy controls. Compared with CHD controls the results showed better exercise capacity in MINCA but lower mental and vitality scores in the mental component of SF-36, otherwise the dimensions were similar. Study 4: The aim was to evaluate mental health in MINCA patients and compare them with two control groups by using two different surveys 3 months after the acute event; the Beck Depression Inventory (BDI) and the Hospital Anxiety and Depression scale (HADS). Our findings showed that anxiety and depression were common with prevalence rates similar to patients with CHD. Anxiety was more common in patients with MINCA and TS than those without TS. Conclusions: CMR imaging is an important tool that can help us to identify the different underlying diagnoses in MINCA and enable a more adequate treatment. Patients with MINCA do not have signs of early or generalized atherosclerosis and they share a number of cardiovascular risk factors with patients who have CHD, including high prevalence of anxiety and depression. There is also a decline in QoL similar to that of CHD patients and in some perspectives even worse in the domain of mental health. Altogether these findings show a high vulnerability to mental stress in patients with MINCA. The lack of clarity regarding diagnosis and treatment can also increase the stress and therefore highlight the need for a change in the management care of patients with MINCA, not only in the hospital but also after being discharged. Performing CMR early (2 weeks from presentation) and follow-up care in in a similar way as in patients with CHD will probably decrease the mental stress and improve QoL.
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5.
  • Eklund, Daniel, 1984- (författare)
  • Mycobacterium tuberculosis and the human macrophage : shifting the balance through inflammasome activation
  • 2013
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • Mycobacterium tuberculosis is a very successful pathogen and tuberculosis constitutes a major threat to global health worldwide. The World Health Organization (WHO) estimates that almost nine million new cases and 1.5 million deaths occur annually and the situation is worsened by increased antibiotic resistance and an extreme synergism with the HIV pandemic. M. tuberculosis primarily affects the lungs where the infection can lead to either eradication of the bacteria or the initiation of an immune response that culminates in the formation of a large cluster of immune cells termed granulomas. In these granulomas, the bacteria can either replicate and cause disease with the ultimate goal of spreading to new hosts or cause latent tuberculosis, which can persist for decades. The tools available to manage the disease are currently suboptimal and include lengthy antibiotic treatments and an inefficient vaccine resulting in poor protection. On a cellular level, M. tuberculosis primarily infects the cell designed to recognize, ingest and eradicate bacteria, namely the human macrophage. Following recognition, the macrophage phagocytoses the bacterium and tries to kill it using an array of different effector mechanisms including acidification of the bacterium-containing vacuole, different degradative enzymes and the generation of radicals. However, the bacterium is able to circumvent many of these harmful effects, leading to a tug-of-war between the bacterium  and host macrophage. This thesis aims at studying the interaction between the human macrophage and M. tuberculosis to identify host factors critical for controlling growth of the bacteria. More specifically, it focuses on the role of an intracellular receptor protein called NLRP3 and its downstream effects. NLRP3 is activated in human macrophages infected by M. tuberculosis and upon activation it forms a multi-protein complex known as the inflammasome. This protein complex is known to induce the production of the proinflammatory cytokine IL-1β and specialized forms of macrophage cell death. We hypothesized that stimulating this pathway would have a beneficial effect for the host macrophage during infection with M. tuberculosis.To allow us to follow interaction between M. tuberculosis and the human macrophage, we first developed a luminometry-based method of measuring bacterial numbers and following bacterial growth over several days in infected cells. With this new assay we showed that low numbers of bacteria induced very low levels of IL-1β and failed to induce any type of cell death in the macrophage. However, when a critical number of bacteria were reached, the infected macrophages underwent necrosis, which was accompanied by high levels of IL-1β. We were also able to show that addition of vitamin D, which has been implicated as an important factor for increased killing capacity of infected macrophages, increased the production of IL-1β, which coincided with increased killing of M. tuberculosis. This effect was seen specifically in cells from patients with active tuberculosis, suggesting that these cells are primed to respond to vitamin D and increased levels of IL-1β. Furthermore, we also showed that increasing production of IL-1β by stimulating infected macrophages with apoptotic neutrophils in turn drives the production of other proinflammatory cytokines. Lastly, we showed that gain-of-function polymorphisms in inflammasome components linked to increased inflammasome activation and IL-1β production promotes bacterial killing in human macrophages. In conclusion, the work presented in this thesis shows  that by enhancing the functions of the inflammasome, it is possible to tip the balance between the human macrophage and M. tuberculosis in favor of the host cell.
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6.
  • Ferm, Lisa, 1984- (författare)
  • Vocational Students’ Agency in Identity Formation as Industrial Workers
  • 2021
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • The overall aim of this thesis is to contribute knowledge about vocational identity formation among students within the industrial programme in Swedish upper secondary education, with a particular focus on their workplace-based learning. To break down the aim, three research questions have been formulated and each is addressed in one or two specific articles. These questions are: (1) What learning strategies do vocational students use to become part of a work community, and how do these strategies relate to the formation of a vocational identity at the workplace? (2) How do vocational students experience their identity formation in relation to a vocation within the industrial sector? (3) How do vocational students handle the division between theoretical and practical knowledge as they learn to become skilled industrial workers? The thesis builds on 53 semi-structured qualitative interviews with Swedish upper secondary vocational students enrolled on the industrial programme. The interviews revolve around the students’ vocational identity formation, with a focus on their workplace-based learning. The students are between 18 and 20 years old and the majority are boys. The findings are analysed through the theoretical lens of situated learning, where identity formation is viewed as a social learning process that takes place through participation in communities of practice. In addition, the concepts of habitus, gender and social categorisation are used as analytical tools to provide a deeper understanding of issues concerning status, power and exclusion in relation to vocational identity formation. The findings reveal that the students’ vocational identity formation is closely connected to the social aspects of participating in workplace communities. Knowledge about the jargon and social norms of the workplace seem to be of more importance for vocational identity formation than knowledge about the concrete working tasks. The study follows the students’ vocational identity formation throughout their vocational learning trajectories, which reveal that vocational identities are formed in heterogenic ways. The students may adopt a committed, flexible or ambivalent approach towards industrial work. Aspects concerning agency and status seem to be crucial for the vocational identification process. The forming of a vocational identity also implies positioning oneself in the hierarchy and division between theoretical and practical knowledge, as well as between masculinity and femininity. The students appear as knowledgeable actors who are aware of the generally low status of industrial work, while simultaneously expressing a great deal of pride in relation to their intended vocations. In the discussion, a model of the students’ vocational identity formation is proposed to capture the interplay between collective and structural dimensions (e.g. social background, class and status hierarchies at school) and students’ agency and strategies in becoming industrial workers. From the findings of this thesis, three main conclusions are drawn: (1) The students form vocational identities through using vocational agency in actively developing strategies for becoming accepted in the workplace community; (2) Workplace-based learning is central for the students’ vocational identity formation, in spite of the relatively short time that the students spend there, compared to the time spent at school; (3) The students’ vocational image awareness, expressed through awareness of, and reactions to, other people’s images of their vocation, constitutes an important part of their vocational identity formation. 
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7.
  • Gonzalez-Castrillon, Luz Maria, 1990- (författare)
  • Molecular mechanisms of nerve-tumor interactions : the intersection of cancer and neurodevelopment
  • 2023
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • A wide range of cancers throughout the body are characterized by high nerve density and invasion of cancer cells within the nerves, a process called perineural invasion (PNI). Work in the field has shown that blocking nerves in organs with tumors leads to improved disease outcomes suggesting that finding ways to block tumor nerves could lead to new treatment approaches. Despite the importance of this, little is known about the molecular mechanisms underlying nerve-tumor interactions. An increasing number of studies have revealed that the genes associated with PNI are classical neurodevelopmental genes associated with neurodevelopmental processes. Therefore, the central hypothesis of this thesis was that nerve-tumor interactions result in part from an abnormal reactivation of the molecular pathways underlying the embryonic development of the nervous system. To test this hypothesis, public datasets from different types of cancer with high incidence of PNI were analyzed to identify molecular pathways common between these cancers. This analysis revealed that neurodevelopmental pathways accounted for 12 - 16% of the differentially expressed genes (DEGs), with axon guidance genes being markedly dysregulated. Overall, 17 different axon guidance gene families, including ephrin-Eph, semaphorin-neuropilin/plexin and slit-robo pathways were dysregulated. Further disruption of these pathways was a common feature across a number of cancers analyzed and their dysregulation had a significant impact on disease survival. Overall, this suggested that neurodevelopmental molecular pathways may contribute to tumor axonogenesis and PNI. These findings suggested a significant role of neurodevelopmental pathways in cancer dysregulation. Thus, a comprehensive understanding of the pathways during the nervous system development is imperative. Therefore, in my thesis, the embryo was used as a tool to study the mechanisms by which these molecular pathways influence axonogenesis more broadly. First, the role of the axon guidance genes Slit/Robo was examined during mouse neurodevelopment. Our results showed that Robo2 enrichment influences the migration and axonal projections of spinal ipsilateral neurons. In parallel, we investigated the role of alternative splicing of transcription factors as a mechanism of increase neuronal diversity. In particular we examined the expression dynamics of Lhx9, a transcription factor that controls the expression of the axon guidance gene Robo3. Lhx9 splice variants showed a differential expression at key developmental points in the spinal cord, suggesting Lhx9 splice dynamics plays an important role in neural guidance choices. In the third part of the thesis, I investigated the role of gap junctions, in nerve-tumor interactions, using pancreatic ductal adenocarcinoma (PDAC) cancer cells in vitro models. The connexin GJB2 emerged as the most overexpressed gap junction component in PDAC tumors. In vitro analysis, involving blocking gap junctions or connexin overexpression, revealed that gap junctions influenced PDAC cancer cell behaviors and properties. Further we developed a novel nerve-tumor assay and used it to examine the role of gap junction genes in PDAC cells neuronal growth. Overall, this thesis postulated that several key molecular pathways crucial for normal nervous system embryonic development, could underly nerve- tumor interactions during cancer development and progression.
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8.
  • Halje, Lovisa (författare)
  • Rättslig operationalisering av företags samhällsansvar : Särskilt om avtalsrätt, köprätt, konsumentköprätt, marknadsföringsrätt och aktiebolagsrätt
  • 2022
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • The main objective of this thesis is to explore and describe the possibilities for legal operationalization of CSR in contract law, sales law and consumer sales law, marketing law and company law. A supplementary aim is to identify regulation on and illustrate the regulatory context of CSR. The illustration provides a background and a framework for the main objective of the study, as well as confirms the legal relevance of CSR.Legal operationalization of CSR requires that CSR is anchored in law and that there are mechanisms for enforcement available. In contract law, CSR is legally anchored when a CSR condition follows from a contract. In a Swedish sales law context, legal anchoring takes place when a buyer’s expectation regarding CSR is not met and this is considered a defect, fel. From a marketing law point of view, the possibilities to consider a CSR statement an unfair commercial practice are relevant. In a company law context, CSR is legally anchored when included in the articles of association, in an instruction from the general meeting or the board, in the board’s internal guidelines or in the remuneration guidelines of the company. In all, contract parties, shareholders and the board are “strong” legal subjects, in that they are able to proactively formulate and anchor CSR norms in law. In company law, the understanding of the company’s interest and purpose, as well as the business case for CSR and the understanding of profit create borders within which the company law operationalization of CSR functions. Sales law and marketing law offer retroactive tools to market actors. The possibilities to anchor CSR in marketing law are vast, whereas the applicability of the sales law rules to a large extent is dependent on the connection between the (failed) CSR expectation and the sold goods. The remedies in the explored areas of law typically do not protect the CSR interest in question directly.
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9.
  • Hjorth, Maria (författare)
  • Daily Experiences and Perceived Quality of Care for Patients with Liver Cirrhosis
  • 2024
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • Aim and methods: This thesis aimed to study patients’ experiences with illness in their day-to-day lives and their perceived quality of care before and after implementing a 24-month adjunctive registered nurse-based outpatient intervention in liver cirrhosis. Qualitative data was used to explore patient perspectives on day-to-day life and healthcare experiences related to liver cirrhosis. The patient-perceived quality of care following the adjunctive registered nurse-based outpatient care was studied in a pragmatic, randomised controlled multicentre study, preceded by a study protocol.Results: Liver cirrhosis led to physical symptoms sometimes appearing rapidly. Fatigue, fear and social stigma affected daily life, resulting in cancelled activities and creating an unpredictable daily life situation. Patients with liver cirrhosis lacked adequate support to learn about the disease and manage it. They sought a trustworthy relationship with healthcare providers. When this was lacking, they felt neglected. After 12 months, the adjunctive registered nurse-based outpatient care revealed an improvement in patient-perceived quality of care. Enhancements were observed in 7 out of 22 questionnaire items regarding: patient participation, access to outpatient care, and feeling understood. However, these improvements were not sustained after 24 months.Conclusions: Fluctuating liver cirrhosis symptoms and constant worry significantly impact patients’ daily lives. Patients expressed a wish to be more involved in their healthcare and support in understanding and managing their illness. Structured registered nurse-based outpatient care for liver cirrhosis could complement physician-based care to meet patient desires for a more person-centred approach, continuity and care coordination. 
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10.
  • Ihse, Cecilia, 1970- (författare)
  • Präst, stånd och stat : Kung och kyrka i förhandling 1642-1686
  • 2005
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • This dissertation is the result of a study of power relations between the crown and the church in Sweden during the 17th century. The study is focused on the Swedish Parliament and how the Estate of the Clergy responded to royal pretensions. The Swedish Clerical Estate is viewed as essential for the Swedish state formation process.The argument in the study is inspired by theories suggesting that state building and state formation were outcomes of a bargaining process between rulers and local power holders. The perspective presented by the historian Jan Glete is of great importance. He defines the early modern state as a complex organization providing protection and violence control. He emphasizes that the power of the state and the state’s character were dependent upon how the state could assert power. In order to do this, the rulers bartered with their subjects using protection as a commodity while in return the subjects paid required taxes. This bargaining process is interpreted as interactive. The rulers linked various local interests to the state and in doing so gained control of the society and the use of violence.The Swedish Clerical Estate played an important role in this process. Due in part to this fact, the clergy differed from the other subjects of the realm such as the nobility or the peasants. The clergy did not own any sizeable amount of property and did not exert any economical influence. Instead the Clerical Estate negotiated using their ideological, cultural and political resources. These commodities became essential in how the king organized the state. In exchange for royal protection, the clergy were given the task of supporting and explaining the crown’s economical and military needs. By doing so, the Clerical Estate legitimated the royal power in the parliament and in the society as a whole. At the same time, this negotiation signified a definition of the role of the clergyman within the state. Though the Clerical Estate sometimes tried to reject royal claims, it was the king who decided the conditions of negotiation. The parliament as a political field was created by the king and for the king. From a political point of view, religion and a theological framework became of great importance and were adopted by the crown in order to exploit resources. Taking this into consideration, the 17th century Swedish state seems to be more effective than other European early modern states.
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