| 1. |
- Arnshav, Mirja, 1977-
(författare)
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De små båtarna och den stora flykten : Arkeologi i spåren av andra världskrigets baltiska flyktbåtar
- 2020
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The Baltic states, Estonia, Latvia and Lithuania, were occupied no less than three times during the Second World War. Faced with a reign of terror, the threat of deportation, and compulsory conscription, over 30,000 people fled over the Baltic Sea to Sweden. On their arrival in Sweden, most escape boats were confiscated by the authorities and in 1945 were returned to the Soviet Union.This study, which began as an attempt to find out about the Baltic escape boats that ended up in Sweden, is inspired by a foreign boat in a small fishing harbour on the island of Gotland. My curiosity was piqued when I caught sight of the boat and heard that it had probably been an escape boat.The purpose of this thesis is to establish which, if any, Baltic escape boats survive in Sweden, in which contexts they remain, and to review their state of preservation, as well as to answer the question of their significance for the memory of the escape. It is an archaeology of the escape and its aftermath, based on the surviving escape boats – what the boats say about the escape, what happened to them afterwards, and how people relate to them and their historical legacy today.The study looks at 35 boats that have been described as Baltic escape boats in various contexts. It shows that they are found in a multitude of environments and in a range of different states of preservation. The boats illustrate experiences that few other source materials can convey, in a manner pertinent to the archaeological understanding of flight. In addition, the boats are rare traces of an earlier Estonian coastal culture eradicated by the second Soviet occupation.One of the most important outcomes of the study is the documentation from the survey and examinations carried out. Few of the boats were known outside their local context. A national compilation has been lacking, which has impeded research and ultimately our understanding of the breadth and complexity of the Swedish historical landscape when it comes to ship remains.
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| 2. |
- Hammarström, Björn, et al.
(författare)
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Acoustic Trapping for Bacteria Identification in Positive Blood Cultures with MALDI-TOF MS.
- 2014
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 1520-6882 .- 0003-2700. ; 86:21, s. 10560-10567
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Tidskriftsartikel (refereegranskat)abstract
- Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is currently changing the clinical routine for identification of microbial pathogens. One important application is the rapid identification of bacteria for the diagnosis of bloodstream infections (BSI). A novel approach based on acoustic trapping and an integrated selective enrichment target (ISET) microchip that improves the sample preparation step for this type of analysis is presented. The method is evaluated on clinically relevant samples in the form of Escherichia coli infected blood cultures. It is shown that noncontact acoustic trapping enables capture, enrichment, and washing of bacteria directly from the complex background of crude blood cultures. The technology replaces centrifugation-based separation with a faster and highly automated sample preparation method that minimizes manual handling of hazardous pathogens. The presented method includes a solid phase extraction step that was optimized for enrichment of the bacterial proteins and peptides that are used for bacterial identification. The acoustic trapping-based assay provided correct identification in 12 out 12 cases of E. coli positive blood cultures with an average score of 2.19 ± 0.09 compared to 1.98 ± 0.08 when using the standard assay. This new technology opens up the possibility to automate and speed up an important and widely used diagnostic assay for bloodstream infections.
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| 3. |
- Hammarström, Björn, et al.
(författare)
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Acoustic trapping with seed-particles for submicron particle enrichment.
- 2012
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Ingår i: Journal of the Acoustical Society of America. - : Acoustical Society of America (ASA). - 1520-8524 .- 0001-4966. ; 132:3
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Tidskriftsartikel (refereegranskat)abstract
- Acoustic trapping in disposable borosilicate capillaries utilize ultrasonic forces to capture/retain micro-particles or cells against fluid flow in a microfluidic-channel. A miniaturized ultrasonic transducer is used to locally excite a 4-MHz cross-sectional resonance in the capillary, creating an acoustic field gradient for retention of cells in non-contact mode. Due to competition between fluidic drag from induced acoustic streaming and primary radiation force the smallest particle size addressable with the trapping system is limited. Here, the typical transition occurs at single-micron particle diameters. However, trapping of single- or sub-micron biological species has highly relevant applications such as enrichment or purification of bacteria or viruses. This work investigates the influence of in-trap particle concentration on the trapping, and it is found that elevated concentrations allow capture of submicron particles. By preloading the acoustic trap with micron-sized seed-particles capture of submicron particles even at low concentrations is enabled. Using this technique, we demonstrate single event capture of bacteria as well as capture of 100nm particles. To provide analytical readout for identification/analysis of the trapped particles the acoustic trap is interfaced with a MALDI-MS instrument. Here, the acoustic trapping capillary is operated in aspirate/dispense mode allowing easy and flexible handling of small sample volumes.
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| 4. |
- Hammarström, Björn, et al.
(författare)
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Efficient sample preparation in immuno-matrix-assisted laser desorption/ionization mass spectrometry using acoustic trapping
- 2013
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Ingår i: Biomicrofluidics. - : AIP Publishing. - 1932-1058. ; 7:2
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Tidskriftsartikel (refereegranskat)abstract
- Acoustic trapping of minute bead amounts against fluid flow allows for easy automation of multiple assay steps, using a convenient aspirate/dispense format. Here, a method based on acoustic trapping that allows sample preparation for immuno-matrix-assisted laser desorption/ionization mass spectrometry using only half a million 2.8 mu m antibody covered beads is presented. The acoustic trapping is done in 200 x 2000 mu m2 glass capillaries and provides highly efficient binding and washing conditions, as shown by complete removal of detergents and sample processing times of 5-10 min. The versatility of the method is demonstrated using an antibody against Angiotensin I (Ang I), a peptide hormone involved in hypotension. Using this model system, the acoustic trapping was efficient in enriching Angiotensin at 400 pM spiked in plasma samples. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4798473]
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| 5. |
- Petruk, Ganna, et al.
(författare)
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Targeting Toll-like receptor-driven systemic inflammation by engineering an innate structural fold into drugs
- 2023
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Ingår i: Nature Communications. - : Springer. - 2041-1723. ; 14, s. 1-20
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Tidskriftsartikel (refereegranskat)abstract
- There is a clinical need for conceptually new treatments that target the excessive activation of inflammatory pathways during systemic infection. Thrombin-derived C-terminal peptides (TCPs) are endogenous anti-infective immunomodulators interfering with CD14-mediated TLR-dependent immune responses. Here we describe the development of a peptide-based compound for systemic use, sHVF18, expressing the evolutionarily conserved innate structural fold of natural TCPs. Using a combination of structure- and in silico-based design, nuclear magnetic resonance spectroscopy, biophysics, mass spectrometry, cellular, and in vivo studies, we here elucidate the structure, CD14 interactions, protease stability, transcriptome profiling, and therapeutic efficacy of sHVF18. The designed peptide displays a conformationally stabilized, protease resistant active innate fold and targets the LPS-binding groove of CD14. In vivo, it shows therapeutic efficacy in experimental models of endotoxin shock in mice and pigs and increases survival in mouse models of systemic polymicrobial infection. The results provide a drug class based on Nature´s own anti-infective principles.
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