| 1. |
- Mu, Yabing, et al.
(författare)
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TRAF6 ubiquitinates TGFβ type I receptor to promote its cleavage and nuclear translocation in cancer
- 2011
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Ingår i: Nature Communications. - London : Macmillan Publishers Limited. - 2041-1723. ; 2:330
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Tidskriftsartikel (refereegranskat)abstract
- Transforming growth factor β (TGFβ) is a pluripotent cytokine promoting epithelial cell plasticity during morphogenesis and tumour progression. TGFβ binding to type II and type I serine/threonine kinase receptors (TβRII and TβRI) causes activation of different intracellular signaling pathways. TβRI is associated with the ubiquitin ligase tumor necrosis factor receptor (TNFR)-associated factor 6 (TRAF6). Here we show that TGFβ, via TRAF6, causes Lys63-linked polyubiquitination of TβRI, promoting cleavage of TβRI by TNF-alpha converting enzyme (TACE), in a PKCζ-dependent manner. The liberated intracellular domain (ICD) of TβRI associates with the transcriptional regulator p300 to activate genes involved in tumour cell invasiveness, such as Snail and MMP2. Moreover, TGFβ-induced invasion of cancer cells is TACE- and PKCζ- dependent and the TβRI ICD is localized in the nuclei of different kinds of tumour cells in tissue sections. Thus, our data reveal a specific role for TβRI in TGFβ mediated tumour invasion.
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| 2. |
- Becirovic-Agic, Mediha, et al.
(författare)
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Time course of decompensation after angiotensin II and high-salt diet in Balb/CJ mice suggests pulmonary hypertension-induced cardiorenal syndrome
- 2019
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Ingår i: American Journal of Physiology. Regulatory Integrative and Comparative Physiology. - : the American Physiological Society. - 0363-6119 .- 1522-1490. ; 316:5, s. R563-R570
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Tidskriftsartikel (refereegranskat)abstract
- The genetic background of a mouse strain determines its susceptibility to disease. C57BL/6J and Balb/CJ are two widely used inbred mouse strains that we found react dramatically differently to angiotensin II and high-salt diet (ANG II + Salt). Balb/CJ show increased mortality associated with anuria and edema formation while C57BL/6J develop arterial hypertension but do not decompensate and die. Clinical symptoms of heart failure in Balb/CJ mice gave the hypothesis that ANG II + Salt impairs cardiac function and induces cardiac remodeling in male Balb/CJ but not in male C57BL/6J mice. To test this hypothesis, we measured cardiac function using echocardiography before treatment and every day for 7 days during treatment with ANG II + Salt. Interestingly, pulsed wave Doppler of pulmonary artery flow indicated increased pulmonary vascular resistance and right ventricle systolic pressure in Balb/CJ mice, already 24 h after ANG II + Salt treatment was started. In addition, Balb/CJ mice showed abnormal diastolic filling indicated by reduced early and late filling and increased isovolumic relaxation time. Furthermore, Balb/CJ exhibited lower cardiac output compared with C57BL/6J even though they retained more sodium and water, as assessed using metabolic cages. Left posterior wall thickness increased during ANG II + Salt treatment but did not differ between the strains. In conclusion, ANG II + Salt treatment causes early restriction of pulmonary flow and reduced left ventricular filling and cardiac output in Balb/CJ, which results in fluid retention and peripheral edema. This makes Balb/CJ a potential model to study the adaptive capacity of the heart for identifying new disease mechanisms and drug targets.
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| 3. |
- Björquist, P, et al.
(författare)
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Plasminogen activator inhibitor type-1 interacts exclusively with the proteinase domain of tissue plasminogen activator.
- 1994
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Ingår i: Biochimica et Biophysica Acta. - 0006-3002 .- 1878-2434. ; 1209:2, s. 191-202
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Tidskriftsartikel (refereegranskat)abstract
- Two different techniques have been used to study the complex formation of recombinant human plasminogen activator inhibitor type-1, PAI-1, with either recombinant human two-chain tissue plasminogen activator, tc tPA (EC 3.4.21.68), or the tPA deletion variants tc K2P, containing the kringle 2 domain and the proteinase domain, and P, containing only the proteinase domain. The same value for Kon, 2.10(7) M-1s-1 for binding of PAI-1 was found for the three tPA forms by direct detection of the complex formation in real time by surface plasmon resonance, BIAcore, or indirectly by monitoring the time course of the inhibition of tPA using the chromogenic substrate N-methylsulfonyl-D-Phe-Gly-Arg-4-pNA-acetate. Apparently, no conformational change is involved in the rate-limiting step, since the kon value was found to be independent of the temperature from 20 to 35 degrees C. By the BIAcore technique, it was found that the complex between PAI-1 and tPA covalently coupled to the surface, was stable at 25 degrees C, since no dissociation was seen in buffer. However, extended treatment with 1 M NH4OH destroyed the complex with t 1/2 = 5 h. The same kon values and complex composition were found by measuring either the binding of tPA to PAI-1 captured on the monoclonal antibody MAI-11 or the binding of PAI-1 to tPA captured on the monoclonal antibody 2:2 B10. Quantification of the complex composition between PAI-1 captured on the monoclonal antibody MAI-11 with either tPA, K2P or P gave a one-to-one ratio with the fraction of active PAI-1, consistent with the results from SDS-PAGE and the specific activity of PAI-1. The complexes of the three tPA forms with PAI-1 captured on a large surface of MAI-11 dissociated more rapidly from MAI-11, with the same apparent koff, kdis, = 2.10(-3) s-1, compared with 0.7-10(-3) s-1 for the dissociation of PAI-1 alone. In consistance, the Kd, calculated from the direct determination of the kon and koff for the association of different form of PAI-1 to a small surface of MAI-11, was found to be higher for PAI-1 in complex with tPA than for free active PAI-1. Apparently, upon complex formation, a change is induced in PAI-1 at the binding epitope for MAI-11.(ABSTRACT TRUNCATED AT 400 WORDS)
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| 4. |
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| 5. |
- Dorell, Åsa, et al.
(författare)
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Experiences With Family Health Conversations at Residential Homes for Older People
- 2016
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Ingår i: Clinical Nursing Research. - : SAGE Publications. - 1054-7738 .- 1552-3799. ; 25:5, s. 560-582
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to highlight family members' experiences of participating in Family Health Conversation (FamHC), based on families in which a family member was living in a residential home for older people. A total of 10 families and 22 family members participated in evaluating family interviews 1 month after participating in FamHC. The interviews were analyzed by qualitative content analysis. The main finding was being a part of FamHC increased family members' insights, understanding, and communication within the family. Getting confirmation from nurses was essential to cope with the new life situation, which also meant that they felt comfortable to partly hand over the responsibility for the older person who moved to the residential home. By being open and expressing their feelings, a bad conscience could be relieved. These findings showed that FamHC could be helpful for family members in adapting to this novel situation.
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| 6. |
- Dressel, Sabrina, et al.
(författare)
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Achieving Social and Ecological Outcomes in Collaborative Environmental Governance : Good Examples from Swedish Moose Management
- 2021
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Ingår i: Sustainability. - : MDPI. - 2071-1050. ; 13:4
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Tidskriftsartikel (refereegranskat)abstract
- Collaborative governance approaches have been suggested as strategies to handle wicked environmental problems. Evaluations have found promising examples of effective natural resource governance, but also highlighted the importance of social-ecological context and institutional design. The aim of this study was to identify factors that contribute to the achievement of social and ecological sustainability within Swedish moose (Alces alces) management. In 2012, a multi-level collaborative governance regime was implemented to decrease conflicts among stakeholders. We carried out semi-structured interviews with six ‘good examples’ (i.e., Moose Management Groups that showed positive social and ecological outcomes). We found that ‘good examples’ collectively identified existing knowledge gaps and management challenges and used their discretionary power to develop procedural arrangements that are adapted to the social-ecological context, their theory of change, and attributes of local actors. This contributed to the creation of bridging social capital and principled engagement across governance levels. Thus, our results indicate the existence of higher-order social learning as well as a positive feedback from within-level collaboration dynamics to between-level collaboration. Furthermore, our study illustrates the importance of institutional flexibility to utilize the existing knowledge across stakeholder groups and to allow for adaptations based on the social learning process.
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| 7. |
- Dressel, Sabrina, et al.
(författare)
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Perceived adaptive capacity within a multi-level governance setting : The role of bonding, bridging, and linking social capital
- 2020
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Ingår i: Environmental Science and Policy. - : Elsevier. - 1462-9011 .- 1873-6416. ; 104, s. 88-97
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Tidskriftsartikel (refereegranskat)abstract
- In 2012 Sweden implemented a collaborative governance regime for managing moose (Alces alces). This was guided by the awareness that decentralization and stakeholder participation can help to reduce conflicts, foster systematic learning, and handle complexity. However, previous research has highlighted that there are no blueprint approaches to the governance and management of natural resources. In this case, diverse multi-use landscapes, ever-changing ungulate populations, and other external stressors (e.g. climate change, wildlife diseases) can create challenges for collaborative institutions. Adaptive capacity is therefore needed as it allows a system and the actors involved to react successfully to social-ecological changes and to develop even in times of no imminent change or risk. Using Swedish moose management as an example of a multi-level governance system, this research assesses the critical determinants of adaptive capacity across levels. We developed and applied a psychometric approach to measure actors’ perceived adaptive capacity on two levels in the management system. A web-based survey was sent to Moose Management Groups (n = 765, response rate = 81 %) and Moose Management Units (n = 1,380, response rate = 71 %). Using structural equation modelling, we assessed the relative importance of governance aspects, different types of social capital, as well as human and financial capital on actors’ perceived adaptive capacity. Linking and bridging social capital in the system had significant impacts on both levels. Actors felt more prepared to handle future challenges in moose management when they perceived benefits through collaborations with levels below and expressed social trust in authorities and the management level above. Besides those similarities between the two levels, fairness was a more important determinant of actors’ perceived adaptive capacity on the lower management level. These results can contribute to a future improvement of the collaborative governance setting by finessing strategic interventions on different levels. Furthermore, our results illustrate the importance of scale when assessing the adaptive capacity of a system.
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| 8. |
- Ericsson, Maria, 1970-, et al.
(författare)
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Factors associated with patient decision time in ST-segment elevation myocardial infarction, in early and late responders : an observational cross-sectional survey study
- 2022
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Ingår i: European Journal of Cardiovascular Nursing. - : Oxford University Press. - 1474-5151 .- 1873-1953. ; 21:7, s. 694-701
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Tidskriftsartikel (refereegranskat)abstract
- AIMS: A short time span from symptom onset to reperfusion is imperative in ST-segment elevation myocardial infarction (STEMI). The aim of this study was to determine factors associated with patient decision time for seeking care in STEMI, particularly how symptoms were experienced and affected patient response.METHODS AND RESULTS: A multicentre cross-sectional self-report survey study was completed at five Swedish hospitals representing geographic diversity. The 521 patients were divided into three groups based on their time to respond to symptoms: early (<20 min), intermediate (20-90 min), and late responders (>90 min). Only one out of five patients both responded early and called an ambulance within 20 min. Believing symptoms were cardiac in origin [odds ratio (OR) 2.60], male sex (OR 2.40), left anterior descending artery as culprit artery (OR 1.77), and bystanders calling an ambulance (OR 4.32) were factors associated with early response and correct action. Associated symptoms such as dyspnoea (OR 1.67) and weakness (OR 1.65) were associated with an early action (<20 min), while chest pain was not independently associated with response time. Cold sweat (OR 0.61) prevented late care-seeking behaviour as did a high symptom burden (OR 0.86).CONCLUSION: Misinterpretation of symptoms delays correct care-seeking behaviour because patient expectations may not be aligned with the experience when stricken by Myocardial infarction. Therefore, it is imperative to continuously enhance public awareness in correct symptom recognition and appropriate care-seeking behaviour and to make efforts to educate individuals at risk for STEMI as well as their next of kin.
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| 9. |
- Ericsson, Maria, et al.
(författare)
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Interaction between tele-nurses and callers with an evolving myocardial infarction : consequences for level of directed care
- 2019
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Ingår i: European Journal of Cardiovascular Nursing. - : Sage Publications. - 1474-5151 .- 1873-1953. ; 18:7, s. 545-553
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Tidskriftsartikel (refereegranskat)abstract
- Background: Rapid contact with emergency medical services is imperative to save the lives of acute myocardial infarction patients. However, many patients turn to a telehealth advisory nurse instead, where the delivery of urgent and safe care largely depends on how the interaction in the call is established.Purpose: The purpose of this study was to explore the interaction between tele-nurses and callers with an evolving myocardial infarction after contacting a national telehealth advisory service number as their first medical contact.Method: Twenty men and 10 women (aged 46–89 years) were included. Authentic calls were analysed using inductive content analysis.Findings: One overall category, Movement towards directed level of care, labelled the whole interaction between the tele-nurse and the caller. Four categories conceptualised the different interactions: a distinct, reasoning, indecisive or irrational interaction. The interactions described how tele-nurses and callers assessed and elaborated on symptoms, context and actions. The interaction was pivotal for progress in the dialogue and affected the achievement of mutual understanding in the communicative process. An indecisive or irrational interaction could increase the risk of failing to recommend or call for acute care.Conclusion: The interaction in the communication could either lead or mislead the level of care directed in the call. This study adds new perspectives to the communicative process in the acute setting in order to identify a myocardial infarction and the level of urgency from both individuals experiencing myocardial infarction and professionals in the health system.
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| 10. |
- Ericsson, Maria, 1970-, et al.
(författare)
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Lärarenkät om de nationella proven i matematik : Kurs B, C och D våren 2004
- 2005
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Av de 329 lärare som besvarat enkäten för Matematik B har 35 % angett att deras elever tillhör samhällsprogrammet och 11 % att deras elever tillhör estetiska programmet. För dessa program är kursprovet i Matematik B obligatoriskt. Av de svarande lärarna tycker 72 % att poänggränsen för betyget Godkänd är lämplig och bland de övriga är det något fler, 18 %, som tycker att den är för låg. När det gäller totalpoänggränsen samt minsta antal vg-poäng för provbetyget Väl godkänd tycker 77 % respektive 82 % att gränserna är lämpliga. 90 % av lärarna tycker att det är bra eller mycket bra att bedömningen sker med både g- och vg-poäng och 75 % anser att provet till ganska eller mycket stor del kan användas som ett viktigt underlag för att sätta kursbetyget Mycket väl godkänd. 80 % tycker till ganska eller mycket stor del att informationen och anvisningarna om hur kursprovet skall användas som underlag för bedömning med betyget Mycket väl godkänd var tillräcklig. Några enkätfrågor behandlar uppgifternas språkliga utformning till exempelvad gäller ordval, meningsbyggnad, textmängd och layout. Av lärarsvaren att döma är ordval och meningsbyggnad mer problematiskt för eleven än textmängd, och layouten upplevs inte som något problem utom i något enstaka fall. Av enkäten framgår att många elever, 59 %, i ganska liten eller mycket liten utsträckning fått ta del av innebörden i de tre aspekter som ligger bakom bedömningen av provets sista uppgift. Det är en minskning jämfört med tidigare år. Bland de positiva allmänna kommentarerna kan nämnas att flera lärare tycker att det är ett mycket bra prov och att provet ger ett bra underlag för att se var G/VG- och VG/MVG-gränserna ligger. Det finns också några negativa omdömen som att provet är för teoretiskt och då särskilt med tanke på elever som går på samhälls- och estetprogrammet. Några kommentarer antyder att det inte finns ett tillräckligt fylligt underlag för betygssteget IG/G, vilket skulle kunna tolkas som att de finns lite för få uppgifter för de elever som ligger nära gränsen för betyget Godkänd.
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