| 1. |
- Edqvist, Per-Henrik D, et al.
(författare)
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Expression of Human Skin-Specific Genes Defined by Transcriptomics and Antibody-Based Profiling
- 2015
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Ingår i: Journal of Histochemistry and Cytochemistry. - : SAGE Publications. - 0022-1554 .- 1551-5044. ; 63:2, s. 129-141
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Tidskriftsartikel (refereegranskat)abstract
- To increase our understanding of skin, it is important to define the molecular constituents of the cell types and epidermal layers that signify normal skin. We have combined a genome-wide transcriptomics analysis, using deep sequencing of mRNA from skin biopsies, with immunohistochemistry-based protein profiling to characterize the landscape of gene and protein expression in normal human skin. The transcriptomics and protein expression data of skin were compared to 26 (RNA) and 44 (protein) other normal tissue types. All 20,050 putative protein-coding genes were classified into categories based on patterns of expression. We found that 417 genes showed elevated expression in skin, with 106 genes expressed at least five-fold higher than that in other tissues. The 106 genes categorized as skin enriched encoded for well-known proteins involved in epidermal differentiation and proteins with unknown functions and expression patterns in skin, including the C1orf68 protein, which showed the highest relative enrichment in skin. In conclusion, we have applied a genome-wide analysis to identify the human skin-specific proteome and map the precise localization of the corresponding proteins in different compartments of the skin, to facilitate further functional studies to explore the molecular repertoire of normal skin and to identify biomarkers related to various skin diseases.
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| 2. |
- Habuka, Masato, et al.
(författare)
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The Urinary Bladder Transcriptome and Proteome Defined by Transcriptomics and Antibody-Based Profiling
- 2015
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Ingår i: PLOS ONE. - : Public Library of Science. - 1932-6203. ; 10:12
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Tidskriftsartikel (refereegranskat)abstract
- To understand functions and diseases of urinary bladder, it is important to define its molecular constituents and their roles in urinary bladder biology. Here, we performed genome-wide deep RNA sequencing analysis of human urinary bladder samples and identified genes upregulated in the urinary bladder by comparing the transcriptome data to those of all other major human tissue types. 90 protein-coding genes were elevated in the urinary bladder, either with enhanced expression uniquely in the urinary bladder or elevated expression together with at least one other tissue (group enriched). We further examined the localization of these proteins by immunohistochemistry and tissue microarrays and 20 of these 90 proteins were localized to the whole urothelium with a majority not yet described in the context of the urinary bladder. Four additional proteins were found specifically in the umbrella cells (Uroplakin 1a, 2, 3a, and 3b), and three in the intermediate/basal cells (KRT17, PCP4L1 and ATP1A4). 61 of the 90 elevated genes have not been previously described in the context of urinary bladder and the corresponding proteins are interesting targets for more in-depth studies. In summary, an integrated omics approach using transcriptomics and antibody-based profiling has been used to define a comprehensive list of proteins elevated in the urinary bladder.
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| 3. |
- Nilsson, Peter, et al.
(författare)
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Metabola syndromet i blåsväder. Internationell strid om riskfaktorkomplexets vetenskapliga grund
- 2005
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Ingår i: Läkartidningen. - 0023-7205. ; 102:44, s. 3-3202
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Tidskriftsartikel (refereegranskat)abstract
- En ansamling av riskfaktorerna bukfetma, hypertoni, dyslipidemi och glukosintolerans på basis av insulinresistens brukar ofta betecknas som metabola syndromet, men denna term är för närvarande omstridd i den internationella vetenskapliga debatten. Oavsett benämning finns denna riskfaktoransamling hos många individer och bör inte ignoreras. Rådgivning och hjälp till en förbättrad livsstil med kost, motion, rökstopp och stresshantering utgör grunden för behandling av detta riskfaktorkomplex.
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