| 1. |
- Das, A., et al.
(författare)
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Surface chemical and adsorption studies using Thiobacillus ferrooxidans with reference to bacterial adhesion to sulfide minerals
- 1999
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Ingår i: Biohydrometallurgy and the Environment toward the Mining of the 21st century. - : Elsevier. - 0444501932 ; , s. 697-707
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Konferensbidrag (refereegranskat)abstract
- Adhesion of Thiobacillus ferrooxidans to pyrite and chalcopyrite in relation to its importance in bioleaching and bioflotation has been studied. Electrokinetic studies as well as FT-IR spectra suggest that the surface chemistry of Thiobacillus ferrooxidans depends on bacterial growth conditions. Sulfur-,Pyrite- and chalcopyrite-grown Thiobacillus ferrooxidans were found to be relatively more hydrophobic. The altered surface chemistry of Thiobacillus ferrooxidans was due to secretion of newer and specific proteinaceous compounds. The adsorption density corresponds to a monolayer coverage in a horizontal orientation of the cells. The xanthate flotation of pyrite in presence of Thiobacillus ferrooxidans is strongly depressed where as the cells have insignificant effect on chalcopyrite flotation. This study demonstrate that:(a)Thiobacillus ferrooxidans cells can be used for selective flotation of chalcopyrite from pyrite and importantly at natural pH values.(b)Sulfur-grown cells exhibits higher leaching kinetics than ferrous ion-grown cells.
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| 2. |
- P.K., Sharma, et al.
(författare)
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Surface characterization of Acidithiobacillus ferrooxidans cells grown under different conditions
- 2003
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Ingår i: Hydrometallurgy. - 0304-386X .- 1879-1158. ; 71:1-2, s. 285-292
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Tidskriftsartikel (refereegranskat)abstract
- The growth characteristics of a strain of Acidithiobacillus ferrooxidans isolated from mine water with ferrous ion, sulfur, or pyrite mineral as sole source of energy have been established. The electrokinetic behaviour of the bacterial cells was investigated and the cell surface groups were characterized by diffuse reflectance FT-IR and FT-Raman spectroscopies. The surface charge on the iron-grown cells (i.e.p., pH 2.0) is different from that on the solid-substrate-grown cells (i.e.p., pH 3.0-3.5) and the surface charge depends on the growth history of the bacteria. The FT-IR and FT-Raman spectra revealed a higher amount of protein content on the surface of the sulfur- and pyrite-grown cells compared to the iron-grown cells. These results suggest that the altered surface charge on the cells is due to differences in the protein content synthesized by bacteria exposed to different growth conditions.
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| 3. |
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| 4. |
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| 6. |
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| 7. |
- Sharma, P.K., et al.
(författare)
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Surface chemical characterisation of Paenibacillus polymyxa before and after adaptation to sulfide minerals
- 2001
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Ingår i: International Journal of Mineral Processing. - 0301-7516 .- 1879-3525. ; 62:1-4, s. 3-25
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Tidskriftsartikel (refereegranskat)abstract
- A heterotroph Paenibacillus polymyxa bacteria is adapted to pyrite, chalcopyrite, galena and sphalerite minerals by repeated subculturing the bacteria in the presence of the mineral until their growth characteristics became similar to the growth in the absence of mineral. The unadapted and adapted bacterial surface have been chemically characterised by zeta-potential, contact angle, adherence to hydrocarbons and FT-IR spectroscopic studies. The surface free energies of bacteria have been calculated by following the equation of state and surface tension component approaches. The aim of the present paper is to understand the changes in surface chemical properties of bacteria during adaptation to sulfide minerals and the projected consequences in bioflotation and bioflocculation processes.The mineral-adapted cells became more hydrophilic as compared to unadapted cells. There are no significant changes in the surface charge of bacteria before and after adaptation, and all the bacteria exhibit an iso-electric point below pH 2.5. The contact angles are observed to be more reliable for hydrophobicity assessment than the adherence to hydrocarbons. The Lifschitz--van der Waals/acid--base approach to calculate surface free energy is found to be relevant for mineral--bacteria interactions. The diffuse reflectance FT-IR absorbance bands for all the bacteria are the same illustrating similar surface chemical composition. However, the intensity of the bands for unadapted and adapted cells is significantly varied and this is due to different amounts of bacterial secretions underlying different growth conditions.
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| 8. |
- Sharma, P.K., et al.
(författare)
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Thiobacillus ferrooxidans interaction with sulfide minerals and selective chalcopyrite flotation from pyrite
- 1999
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Ingår i: Advances in flotation technology. - : Society for Mining, Metalurgy and Exploration. - 0873351843 ; , s. 147-165
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Konferensbidrag (refereegranskat)abstract
- The acidophilic and chemolithotrophic Thiobacillus ferrooxidans bacteria grown in the presence of soluble ferrous ions and solid substrates (sulfur, pyrite and chalcopyrite) as their energy source have been used in the investigations. The cell surfaces are characterized by zeta-potential, contact angle, FT-IR and FT-Raman studies. The adsorption isotherms of the cells on pyrite and chalcopyrite indicate that the adsorption begins on pyrite at a much lower equilibrium cell population than on chalcopyrite. The isotherms tend to level off at a cell adsorption density corresponding to a horizontal monolayer coverage when the geometrical dimensions of the cells are considered. In the presence of cells, the xanthate flotation of pyrite completely depressed, whereas chalcopyrite flotation is unaffected. The results suggest that chalcopyrite can be selectively floated from pyrite, also at a neutral pH region, in the presence of T. ferrooxidans cells
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