Sound, Light and Electricity : as applications and analysis techniques to study metabolic effect and biofilm characterization of Geobacter sulfurreducens

• ElectricityBio-electrochemical systems such as microbial fuel cells (MFCs) and microbial electrolysis cells have shown promise in wastewater treatment, bioremediation, desalination, carbon sequestration and as an alternative, renewable energy source. MFCs produces electricity via anaerobic oxidation of substrates with the subsequent extracellular electron transfer to an electrode. A wide variety of feedstocks have been researched, including various artificial and real wastewater sources as well as lignocellulosic material. Sweet sorghum, has been identified as a possible feedstock for electricity production in MFCs, using an anaerobic sludge inoculum, due to its high sugar content. To study sweet sorghum as an MFC feedstock a standard two chamber H-cell MFC was used, with an anaerobic sludge inoculum (Boden Biogas). A maximum voltage of 546±10 mV was obtained, and a maximum power and current density of 131±8 mW/m2 and 543±29 mA/m2 respectively. The substrate concentrations were monitored during the MFC operation, and the sugars were quickly fermented to volatile fatty acids which were then consumed during electricity generation. The power output was essentially independent of the substrate profile, with little difference between different VFAs. A more direct way was therefore needed to monitor the growth of an MFC biofilm as well as the effect of various substrates on extracellular electron transfer (EET). LightOne option for the direct monitoring of a biofilm is to use Raman spectroscopy to monitor the redox status of the biofilm, since Raman can be used to detect the redox state heme groups. Therefore, resonance Raman spectroscopy was chosen to monitor the cytochrome redox of Geobacter sulfurreducens, is a well know electroactive microorganism commonly found in mixed culture MFCs. G. sulfurreducensis able to produce thick, conductive biofilms as well as high current densities in MFCs. Due to the large variety of cytochromes present in G. sulfurreducens, it has various intricate and adaptable EET pathways, which makes the characterization of the essential EET components difficult. Due to the resonance of the cytochromes found in G. sulfurreducens it is possible to measure the redox state of the biofilm using resonance Raman spectroscopy. This was used for on-line monitoring of various G. sulfurreducens mutants during MFC operation (including the wild type PCA, the ii enhanced KN400 strain capable of higher current densities, and two deficient strains missing key cytochromes involved in the EET, i.e. ΔOmcS and ΔpilA). From this, the applicability of resonance Raman spectroscopy was shown to provide a non-destructive analytical tool for the in-situ monitoring of the oxidation state of proteins responsible for the EET process and the dynamics thereof. Resonance Raman with short integration times was further used, along with a dynamic model, to describe the dynamics of the EET pathways in the wild type as well as in an OmcS deficient strain during a stepped chronoamperometry measurement. This showed a significant difference in EET dynamics between ΔOmcS and the wild type, which was not detectible in the chronoamperometry data alone. The ΔOmcS biofilm showed a linearly decreasing trend in the reduced cytochrome concentration. This was likely caused by the saturation of a limiting mediator, resulting in an oxidation rate that was independent of the mediator concentration. The ΔOmcS biofilms response could, however, be better modelled using an empirical zeroth order model. This analytical method could prove valuable for the establishment of G. sulfurreducens as a chassis microorganism, allowing one to observe the effect of genetic modification on EET mechanisms.Sound Furthermore, to see if an abiotic factor such as sound can affect the functions in bacterial cells, we selected to study the effect of ultrasound on the growth of G. sulfurreducens. G. sulfurreducens is a key candidate for the development of a chassis organism in bioelectrochemical systems, and an external abiotic method of affecting growth or metabolite production could be extremely beneficial. For this, a well-defined sonobioreactor was developed and modelled to study the effect of ultrasound on G. sulfurreducens. This resulted in a significant increase in malate production during the exponential phase of planktonic growth (11 mmol when sonicated vs the 5 mmol control). Transcriptomics was then used to determine the reason for the observed increase. Although there was a large variance in the samples, this was possibly linked to the overexpression of glycosyltransferases, which are known to play a role in membrane stability and bind malate. Finally, a low-cost modification, which modifies a standard 3D printer into a bio-printer was developed to print artificial biofilms for bio-electrochemical systems. This was then used to print an artificial biofilm of G. sulfurreducens, significantly reducing the time required to produce an established biofilm

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

TEKNIK OCH TEKNOLOGIER  -- Industriell bioteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Industrial Biotechnology (hsv//eng)

Nyckelord

MFC

Microbial fuel cell

Raman microscopy

BES

Geobacter sulfurreducens

Biokemisk processteknik

Biochemical Process Engineering

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