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- Jacobsen, Kevin, et al.
(författare)
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Interobserver agreement on line-field confocal optical coherence tomography image markers in keratinocyte carcinomas and precursor lesions
- 2024
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Ingår i: Archives of Dermatological Research. - 1432-069X. ; 316
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Tidskriftsartikel (refereegranskat)abstract
- Line-field confocal optical coherence tomography (LC-OCT) is a new technology for skin cancer diagnostics. However, the interobserver agreement (IOA) of known image markers of keratinocyte carcinomas (KC), including basal cell carcinoma (BCC) and squamous cell carcinoma (SCC), as well as precursors, SCC in situ (CIS) and actinic keratosis (AK), remains unexplored. This study determined IOA on the presence or absence of 10 key LC-OCT image markers of KC and precursors, among evaluators new to LC-OCT with different levels of dermatologic imaging experience. Secondly, the frequency and association between reported image markers and lesion types, was determined. Six evaluators blinded to histopathologic diagnoses, assessed 75 LC-OCT images of KC (21 SCC; 21 BCC), CIS (12), and AK (21). For each image, evaluators independently reported the presence or absence of 10 predefined key image markers of KCs and precursors described in an LC-OCT literature review. Evaluators were stratified by experience-level as experienced (3) or novices (3) based on previous OCT and reflectance confocal microscopy usage. IOA was tested for all groups, using Conger's kappa coefficient (κ). The frequency of reported image marker and their association with lesion-types, were calculated as proportions and odds ratios (OR), respectively. Overall IOA was highest for the image markers lobules (κ = 0.68, 95% confidence interval (CI) 0.57;0.78) and clefting (κ = 0.63, CI 0.52;0.74), typically seen in BCC (94%;OR 143.2 and 158.7, respectively, p < 0.001), followed by severe dysplasia (κ = 0.42, CI 0.31;0.53), observed primarily in CIS (79%;OR 7.1, p < 0.001). The remaining seven image-markers had lower IOA (κ = 0.06-0.32) and were more evenly observed across lesion types. The lowest IOA was noted for a well-defined (κ = 0.07, CI 0;0.15) and interrupted dermal-epidermal junction (DEJ) (κ = 0.06, CI -0.002;0.13). IOA was higher for all image markers among experienced evaluators versus novices. This study shows varying IOA for 10 key image markers of KC and precursors in LC-OCT images among evaluators new to the technology. IOA was highest for the assessments of lobules, clefting, and severe dysplasia while lowest for the assessment of the DEJ integrity.
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| 2. |
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| 3. |
- Hedenborg, Susanna, et al.
(författare)
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Outdoorification of sports and recreation : a leisure transformation under the COVID-19 pandemic in Sweden
- 2024
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Ingår i: Annals of Leisure Research. - : Informa UK Limited. - 1174-5398 .- 2159-6816. ; 27:1, s. 36-54
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Tidskriftsartikel (refereegranskat)abstract
- Following the outbreak of the COVID-19 pandemic, most European countries imposed lockdowns, whereas Sweden introduced soft restrictions. Sports and physical activity could continue if conducted 'safely' and outdoor activities were even promoted if restrictions on the number of participants were not violated. The aim of this article is to demonstrate how the pandemic led to transitions and transformations of typical indoor sports activities to the outdoors and to outdoor recreation, or what we call an outdoorification process of sports and recreation, and how the changes were perceived by the population. Sweden is used as a case study. The inquiry is based on three studies involving more than 100 semi-structured interviews, two national questionnaire surveys and a regional-based PPGIS study. Based on the results, we argue that the outdoorification process is likely to influence how sport and recreation is understood and practiced also in the years after the pandemic.
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| 4. |
- Sandell, Klas, et al.
(författare)
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Framtida forskningsstrukturer för friluftsliv och naturturism i Sverige - Ett diskussionsunderlag : Forskningsprogrammet Friluftsliv i förändring, rapport nr. 17
- 2011
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- En av uppgifterna som forskningsprogrammet "Friluftsliv i Förändring" (FiF) har är att ta fram ett diskussions- och beslutsunderlag angående framtida forskningsstrukturer för friluftsliv och naturturism i Sverige. Den här rapporten sammanfattar det underlag som hittills har samlats in i form av workshops, presentationer och diskussioner i olika konstellationer. Detta diskussionsunderlag kommer att vara en plattform för det fortsatta arbetet som slutligen kommer att avrapporteras hösten 2012. Utifrån de underlag som hittills samlats in konstateras i rapporten bl.a.: att friluftslivet i förhållande till sin samhällsroll och forskningspotential i övrigt är ett starkt underbeforskat fält i Sverige och att FiF därför måste ses som en inventerande startpunkt – inte en slutpunkt – i förhållande till forskningsbehoven. Detta intryck förstärks av att den då aktuella utlysningen innehöll medvetna avgränsningar samt att ett uttalat syfte för detta första större forskningsprogram om friluftsliv i Sverige var att etablera grundläggande kunskaper om fältet som en bas för fortsatt forskning. Utifrån dessa konstateranden identifieras och diskuteras ett antal val och handlingsalternativ. Rapporten avslutas med en serie preliminära slutsatser varifrån bl.a. följande kan noteras: ␣ att det är viktigt att bevaka ämnesfältets bredd och mångvetenskapliga karaktär; ␣ att en långsiktigt stabil förvaltning av nu uppbyggda kommunikationsstrukturer i form av årliga konferenser, hemsida, informationsbaser och populärvetenskaplig kunskapsspridning är central, liksom att etablera en långsiktigt stabil insamling och kontextualisering av statistik; ␣ att den institutionella strukturen sannolikt bäst byggs upp som ett nätverk av en handfull tematiska nationella centra tillsammans med en nationellt koordinerande centralnod som har särskilt ansvar för kommunikationsstrukturer och statistikinsamling enligt ovan; ␣ att en basfinansierad nationell mångvetenskaplig forskarskola bör inrättas; och ␣ att FiFs ekonomiska omsättning (ca 6 Mkr/år) bör ses som en miniminivå, 10-15 Mkr/år som mer rimligt, och ett antal särskilt angelägna forskningsområden indikeras likaså.
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| 5. |
- Örtegren, Unn, 1975-, et al.
(författare)
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Lipids and glycosphingolipids in caveolae and surrounding plasma membrane of primary rat adipocytes
- 2004
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Ingår i: Eur J Biochem. - : Wiley. - 0014-2956 .- 1432-1033. ; 271:10, s. 2028-36
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Tidskriftsartikel (refereegranskat)abstract
- We have made a comprehensive and quantitative analysis of the lipid composition of caveolae from primary rat fat cells and compared the composition of plasma membrane inside and outside caveolae. We isolated caveolae from purified plasma membranes using ultrasonication in carbonate buffer to disrupt the membrane, or extraction with nonionic detergent, followed by density gradient ultracentrifugation. The carbonate-isolated caveolae fraction was further immunopurified using caveolin antibodies. Carbonate-isolated caveolae were enriched in cholesterol and sphingomyelin, and the concentration was three- and twofold higher, respectively, in caveolae compared to the surrounding plasma membrane. The concentration of glycerophospholipids was similar suggesting that glycerophospholipids constitute a constant core throughout the plasma membrane. The composition of detergent-insoluble fractions of the plasma membrane was very variable between preparations, but strongly enriched in sphingomyelin and depleted of glycerophospholipids compared to carbonate-isolated caveolae; indicating that detergent extraction is not a suitable technique for caveolae preparation. An average adipocyte caveola contained about 22 x 10(3) molecules of cholesterol, 7.5 x 10(3) of sphingomyelin and 23 x 10(3) of glycerophospholipid. The glycosphingolipid GD3 was highly enriched in caveolae, whereas GM3, GM1 and GD1a were present inside as well as outside the caveolae membrane. GD1b, GT1b, GM2, GQ1b, sulfatide and lactosylceramide sulfate were not detected in caveolae.
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