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- Choma, Michal, et al.
(författare)
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Recovery of the ectomycorrhizal community after termination of long-term nitrogen fertilisation of a boreal Norway spruce forest
- 2017
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Ingår i: Fungal Ecology. - : Elsevier BV. - 1754-5048 .- 1878-0083. ; 29, s. 116-122
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Tidskriftsartikel (refereegranskat)abstract
- © 2016 Elsevier Ltd and British Mycological Society.Ectomycorrhizal fungi (ECM) are a fundamental component of boreal forests promoting tree growth and participating in soil nutrient cycling. Increased nitrogen (N) input is known to largely influence ECM communities but their potential recovery is not well understood. Therefore, we studied the effects of long-term N-fertilisation on ECM communities, and their recovery after termination of N treatment. Fungal ITS sequencing data indicated that N-fertilisation (34 kg N ha-1 y-1) for 46 y decreased the relative abundance of ECM species in the fungal community and suppressed originally dominating medium-distance fringe exploration types adapted to N-limited conditions, while the ECM diversity remained unaffected. In other plots, 23 y after termination of fertilisation at 73 kg N ha-1 y-1 for 23 y, the relative abundance of ECM species shifted closer to, but did not reach, control levels. These observations indicate only slow recovery of ECM community, likely due to a high soil N retention capacity.
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| 3. |
- Rappe George, Martin, et al.
(författare)
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Indications that long-term nitrogen loading limits carbon resources for soil microbes
- 2017
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Ingår i: Soil Biology and Biochemistry. - : Elsevier BV. - 0038-0717 .- 1879-3428. ; 115, s. 310-321
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Tidskriftsartikel (refereegranskat)abstract
- Microbial communities in the organic horizon (O-horizon) of forest soils play key roles in terrestrial nitrogen (N) cycling, but effects on them of long-term high N loading, by N deposition or experimental addition, are not fully understood. Thus, we investigated N-loading effects on soil microbial biomass N, carbon (C) and phosphorus stoichiometry, hydrolytic and oxidative enzymes, community composition (via phospholipid fatty acids, PLFA) and soil chemistry of the O-horizon in study plots of three well-studied experimental Norway spruce (Picea abies) forests in Sweden and the Czech Republic. These forests span substantial gradients in current N deposition, experimental N addition and nitrate (NO3 −) leaching. Current N deposition ranges from ∼3 kg ha−1 year−1 of N in central Sweden (Stråsan) to ∼15 kg ha−1 year−1 of N in SW Sweden (Skogaby) and Czech Republic (Čertovo). Furthermore, accumulated historical N loading during 1950–2000 (which include experimental N addition performed at Stråsan and Skogaby) ranged ∼200–∼2000 kg ha−1 of N. Across all sites and treatments, current NO3 − leaching ranged from low (∼0.1 kg ha−1 year−1 of N) at Stråsan, to high (∼15 kg ha−1 year−1 of N) at Skogaby and Čertovo. We found significantly lower C/N ratios and greater amounts of extractable inorganic N species in the forest soils’ O-horizons at the high N loading plots. Microbial biomass and basal respiration decreased under experimental N addition treatments and tended to decrease with increased N deposition. Similarly, activities of hydrolytic enzyme activity associated with N acquisition were lower, although differences in activities at specific sites with the highest and intermediate historical N deposition levels failed statistical significance. Conversely, activities of soil hydrolytic enzymes associated with C acquisition were greater in study plots exposed high N loading. PLFA profiles indicated shifts in microbial community composition induced by long-term N load, towards higher and lower relative abundance of Gram-positive and Gram-negative bacteria, respectively (but no changes in fungal relative abundance). Taken together, our results suggest that long-term N loading of N-limited Norway spruce forests aggravates limitation of other resources, likely of C, for soil microbial communities. Although microbial variables in the soil O-horizon differed between plots exposed to low and high current N loading, microbial variables in plots that leached small amounts and large amounts of NO3 − exposed to high N load were similar. © 2017
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