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Träfflista för sökning "WFRF:(George Anne) ;conttype:(refereed);lar1:(slu)"

Search: WFRF:(George Anne) > Peer-reviewed > Swedish University of Agricultural Sciences

  • Result 1-5 of 5
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2.
  • Klionsky, Daniel J., et al. (author)
  • Guidelines for the use and interpretation of assays for monitoring autophagy
  • 2012
  • In: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
  • Research review (peer-reviewed)abstract
    • In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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3.
  • Kattge, Jens, et al. (author)
  • TRY plant trait database - enhanced coverage and open access
  • 2020
  • In: Global Change Biology. - : Wiley-Blackwell. - 1354-1013 .- 1365-2486. ; 26:1, s. 119-188
  • Journal article (peer-reviewed)abstract
    • Plant traits-the morphological, anatomical, physiological, biochemical and phenological characteristics of plants-determine how plants respond to environmental factors, affect other trophic levels, and influence ecosystem properties and their benefits and detriments to people. Plant trait data thus represent the basis for a vast area of research spanning from evolutionary biology, community and functional ecology, to biodiversity conservation, ecosystem and landscape management, restoration, biogeography and earth system modelling. Since its foundation in 2007, the TRY database of plant traits has grown continuously. It now provides unprecedented data coverage under an open access data policy and is the main plant trait database used by the research community worldwide. Increasingly, the TRY database also supports new frontiers of trait-based plant research, including the identification of data gaps and the subsequent mobilization or measurement of new data. To support this development, in this article we evaluate the extent of the trait data compiled in TRY and analyse emerging patterns of data coverage and representativeness. Best species coverage is achieved for categorical traits-almost complete coverage for 'plant growth form'. However, most traits relevant for ecology and vegetation modelling are characterized by continuous intraspecific variation and trait-environmental relationships. These traits have to be measured on individual plants in their respective environment. Despite unprecedented data coverage, we observe a humbling lack of completeness and representativeness of these continuous traits in many aspects. We, therefore, conclude that reducing data gaps and biases in the TRY database remains a key challenge and requires a coordinated approach to data mobilization and trait measurements. This can only be achieved in collaboration with other initiatives.
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4.
  • Phillips, Helen R. P., et al. (author)
  • Global distribution of earthworm diversity
  • 2019
  • In: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 366:6464, s. 480-
  • Journal article (peer-reviewed)abstract
    • Soil organisms, including earthworms, are a key component of terrestrial ecosystems. However, little is known about their diversity, their distribution, and the threats affecting them. We compiled a global dataset of sampled earthworm communities from 6928 sites in 57 countries as a basis for predicting patterns in earthworm diversity, abundance, and biomass. We found that local species richness and abundance typically peaked at higher latitudes, displaying patterns opposite to those observed in aboveground organisms. However, high species dissimilarity across tropical locations may cause diversity across the entirety of the tropics to be higher than elsewhere. Climate variables were found to be more important in shaping earthworm communities than soil properties or habitat cover. These findings suggest that climate change may have serious implications for earthworm communities and for the functions they provide.
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5.
  • Rebmann, Corinna, et al. (author)
  • ICOS eddy covariance flux-station site setup : A review
  • 2018
  • In: International Agrophysics. - : Walter de Gruyter GmbH. - 0236-8722 .- 2300-8725. ; 32:4, s. 471-494
  • Research review (peer-reviewed)abstract
    • The Integrated Carbon Observation System Research Infrastructure aims to provide long-Term, continuous observations of sources and sinks of greenhouse gases such as carbon dioxide, methane, nitrous oxide, and water vapour. At ICOS ecosystem stations, the principal technique for measurements of ecosystem-Atmosphere exchange of GHGs is the eddy-covariance technique. The establishment and setup of an eddy-covariance tower have to be carefully reasoned to ensure high quality flux measurements being representative of the investigated ecosystem and comparable to measurements at other stations. To fulfill the requirements needed for flux determination with the eddy-covariance technique, variations in GHG concentrations have to be measured at high frequency, simultaneously with the wind velocity, in order to fully capture turbulent fluctuations. This requires the use of high-frequency gas analysers and ultrasonic anemometers. In addition, to analyse flux data with respect to environmental conditions but also to enable corrections in the post-processing procedures, it is necessary to measure additional abiotic variables in close vicinity to the flux measurements. Here we describe the standards the ICOS ecosystem station network has adopted for GHG flux measurements with respect to the setup of instrumentation on towers to maximize measurement precision and accuracy while allowing for flexibility in order to observe specific ecosystem features.
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  • Result 1-5 of 5
Type of publication
journal article (3)
research review (2)
Type of content
Author/Editor
Wang, Mei (2)
Kominami, Eiki (2)
Bonaldo, Paolo (2)
Minucci, Saverio (2)
De Milito, Angelo (2)
Kågedal, Katarina (2)
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Liu, Wei (2)
Clarke, Robert (2)
Kumar, Ashok (2)
Brest, Patrick (2)
Simon, Hans-Uwe (2)
Mograbi, Baharia (2)
Melino, Gerry (2)
Albert, Matthew L (2)
Lopez-Otin, Carlos (2)
Liu, Bo (2)
Ghavami, Saeid (2)
Harris, James (2)
Rillig, Matthias C. (2)
Zhang, Hong (2)
Zorzano, Antonio (2)
Bozhkov, Peter (2)
Petersen, Morten (2)
Przyklenk, Karin (2)
Noda, Takeshi (2)
Zhao, Ying (2)
Kampinga, Harm H. (2)
Zhang, Lin (2)
Harris, Adrian L. (2)
Hill, Joseph A. (2)
Tannous, Bakhos A (2)
Segura-Aguilar, Juan (2)
Dikic, Ivan (2)
Kaminskyy, Vitaliy O ... (2)
Nishino, Ichizo (2)
Okamoto, Koji (2)
Olsson, Stefan (2)
Layfield, Robert (2)
Schorderet, Daniel F ... (2)
Hofman, Paul (2)
Xu, Liang (2)
Sood, Anil K (2)
Yue, Zhenyu (2)
Corbalan, Ramon (2)
Swanton, Charles (2)
Johansen, Terje (2)
Ray, Swapan K. (2)
Nguyen, Huu Phuc (2)
Chevet, Eric (2)
Behl, Christian (2)
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University
University of Gothenburg (2)
Umeå University (2)
Stockholm University (2)
Linköping University (2)
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Karolinska Institutet (2)
Karlstad University (1)
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Language
English (5)
Research subject (UKÄ/SCB)
Natural sciences (5)
Medical and Health Sciences (2)

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