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- Calus, L, et al.
(författare)
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Reply: To PMID 23021878
- 2013
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Ingår i: The Journal of allergy and clinical immunology. - : Elsevier BV. - 1097-6825 .- 0091-6749. ; 132:1, s. 247-248
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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- Calus, L, et al.
(författare)
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IL-21 Is Increased in Nasal Polyposis and after Stimulation with Staphylococcus aureus Enterotoxin B
- 2017
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Ingår i: International archives of allergy and immunology. - : S. Karger AG. - 1423-0097 .- 1018-2438. ; 174:3-4, s. 161-169
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Tidskriftsartikel (refereegranskat)abstract
- <b><i>Background:</i></b> Chronic rhinosinusitis with nasal polyposis (CRSwNP) is an inflammatory disease associated with lymphoid aggregates and local IgE production related to <i>Staphylococcus aureus</i> enterotoxins. T-follicular helper cells and their effector cytokine interleukin (IL)-21 play an important role in germinal center proliferation. <b><i>Methods:</i></b> IL-21 was determined on the mRNA level by qPCR in nasal tissue of 3 groups of patients: control (<i>n</i> = 17), chronic rhinosinusitis without nasal polyposis (CRSsNP; <i>n</i> = 23), and CRSwNP (<i>n</i> = 35). The expression of IL-21 by CD4+ T cells was analyzed in tissue at baseline and after 24-h stimulation of tissue fragments with <i>S. aureus</i> enterotoxin B (SEB) using flow cytometry. Finally, human nasal IL-21+CXCR5+CD4+ T cells were isolated and coincubated with human blood naive B cells to investigate their functionality. <b><i>Results:</i></b> IL-21 mRNA expression was increased in the CRSwNP group (<i>p</i> < 0.05) compared to the control group, and B-cell lymphoma-6 and B-lymphocyte-induced maturation protein-1 were upregulated in CRSwNP versus CRSsNP. Furthermore, SEB was able to increase IL-21 mRNA expression significantly (<i>p</i> < 0.01) in nasal polyps. Flow cytometry revealed that the source of IL-21 was predominantly CD4+ T cells and that IL-21+CD4+ T cells were significantly increased in polyp tissue and further increased after SEB stimulation. Finally, tissue CXCR5+CD4+ T cells derived from nasal polyp tissue were able to induce maturation of human naive B cells. <b><i>Conclusions:</i></b> IL-21- and IL-21-producing CD4+ T cells were increased in CRSwNP. In addition, SEB induced an increase in IL-21 and IL-21+CD4+ T cells, suggesting that <i>S. aureus</i> can modulate the function of Tfh cells in nasal polyps. We speculate that T-follicular helper cells and IL-21 are important in the pathophysiology of CRSwNP.
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- De Schryver, E, et al.
(författare)
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The quest for autoreactive antibodies in nasal polyps
- 2016
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Ingår i: The Journal of allergy and clinical immunology. - : Elsevier BV. - 1097-6825 .- 0091-6749. ; 138:3, s. 893-
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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