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Sentinel-base DNA g...
Sentinel-base DNA genotyping using multiple sequencing primers for high-risk human papillomaviruses
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- Gharizadeh, Baback (författare)
- Stanford Univ, Stanford Genome Technol Ctr
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- Zheng, Biying (författare)
- Karolinska Inst, Dept Mol Med
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- Akhras, Michael (författare)
- KTH,Skolan för bioteknologi (BIO)
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- Ghaderi, Mehran (författare)
- Karolinska Institutet
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- Jejelowo, Olufisayo (författare)
- Texas So Univ
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- Strander, Björn, 1952 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences,Gothenburg Univ, Sahlgrens Acad, Ctr Oncol
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- Nyrén, Pål (författare)
- KTH,Skolan för bioteknologi (BIO)
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- Wallin, Keng-Ling (författare)
- Karolinska Institutet
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- Pourmand, Nader (författare)
- Stanford Univ, Stanford Genome Technol Ctr
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(creator_code:org_t)
- Elsevier BV, 2006
- 2006
- Engelska.
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Ingår i: Mol Cell Probes. - : Elsevier BV. ; 20:3-4, s. 230-238
- Relaterad länk:
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https://europepmc.or...
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https://gup.ub.gu.se...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Despite the various technologies in place for genotyping human papillomaviruses (HPV), clinical use and clinical research demand a method that is fast, more reliable and cost-effective. The technology described here represents a breakthrough development in that direction. By combining the method of multiple sequencing primers with DNA sequencing, we have developed a rapid assay for genotyping HPV that relies on the identification of a single, type-specific 'sentinel' base. As described here, the prototype assay has been developed to recognize the 12 most high-risk HPV types (HPV-16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58 and 59) and is capable of recognizing and simultaneously genotyping multiple HPV co-infections. By providing sequence information on multiple HPV infections, this method eliminates the need for labor- and cost-intensive PCR cloning. These proof-of-concept studies establish the assay to be accurate, reliable, rapid, flexible, and cost-effective, providing evidence of the feasibility this technique for use in clinical settings.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Cervical Intraepithelial Neoplasia/blood/diagnosis/*virology
- DNA Primers/biosynthesis/*genetics
- DNA Probes
- HPV/genetics
- DNA
- Viral/blood/genetics
- Female
- Genotype
- Humans
- Papillomaviridae/classification/*genetics/isolation & purification
- Papillomavirus Infections/blood/diagnosis/*virology
- Polymerase Chain Reaction
- Risk Factors
- Sensitivity and Specificity
- human papillomaviruses (HPV); DNA sequencing; multiple infections; multiple sequencing primers; sentinel-base DNA sequencing; pyrosequencing technology; POLYMERASE-CHAIN-REACTION; GENITAL HUMAN PAPILLOMAVIRUSES; CERVICAL-CANCER; HYBRID CAPTURE; GENERAL PRIMERS; NESTED PCR; SPECIMENS; SAMPLES; HPV; IDENTIFICATION
- Biochemistry
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Gharizadeh, Baba ...
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Zheng, Biying
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Akhras, Michael
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Ghaderi, Mehran
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Jejelowo, Olufis ...
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Strander, Björn, ...
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visa fler...
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Nyrén, Pål
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Wallin, Keng-Lin ...
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Pourmand, Nader
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