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Simultaneous genera...
Simultaneous generation of cytomegalovirus-specific CD8+ and CD4+ T lymphocytes by use of dendritic cells comodified with pp65 mRNA and pp65 protein
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- Carlsson, Björn (författare)
- Uppsala universitet,Enheten för klinisk immunologi
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- Hou, Mingyan (författare)
- Uppsala universitet,Enheten för klinisk immunologi
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- Giandomenico, Valeria (författare)
- Uppsala universitet,Enheten för klinisk immunologi
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- Nilsson, Berith (författare)
- Uppsala universitet,Enheten för klinisk immunologi
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- Tötterman, Thomas H. (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi
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- Essand, Magnus (författare)
- Uppsala universitet,Enheten för klinisk immunologi
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(creator_code:org_t)
- Oxford University Press (OUP), 2005
- 2005
- Engelska.
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Ingår i: Journal of Infectious Diseases. - : Oxford University Press (OUP). - 0022-1899 .- 1537-6613. ; 192:11, s. 1912-20
- Relaterad länk:
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Cytomegalovirus (CMV) disease remains a severe complication in patients who have undergone transplantation. Viremia can be prevented and treated by the adoptive transfer of donor-derived CMV-directed T cells. To ensure long-term protection against CMV disease, it is important to transfer CMV antigen-specific T cells that represent both the CD8+ and the CD4+ subsets. In the present study, we used as stimulators dendritic cells (DCs) that were electroporated with in vitro-transcribed 5'-capped polyadenylated messenger RNA (mRNA) that encoded the CMV pp65 protein (i.e., pp65 mRNA). These DCs could efficiently activate CMV-directed CD8+ T cells, as assayed by tetramer staining, interferon- gamma production, and cytolytic activity. We also used DCs that were pulsed with a recombinant pp65 protein to activate CMV-directed CD4+ T cells. When DCs were comodified with pp65 mRNA and pp65 protein, large numbers of CMV-directed CD8+ and CD4+ T cells were generated simultaneously. The approach outlined in the present study can be adapted for a clinical protocol that circumvents potential virus-related biohazards and is available to all patients independently of their human leukocyte antigen haplotype.
Nyckelord
- MEDICINE
- MEDICIN
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- ref (ämneskategori)
- art (ämneskategori)
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