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- Benjamin, Daniel J., et al.
(författare)
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Redefine statistical significance
- 2018
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Ingår i: Nature Human Behaviour. - : Nature Research (part of Springer Nature). - 2397-3374. ; 2:1, s. 6-10
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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- Conze, Lei Liu, et al.
(författare)
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Construction and deep sequencing of cDNA libraries representing medium-sized RNA
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Transcriptome analysis has recently become feasible with the application of RNA-seq, the high-throughput sequencing analysis of RNAs through cDNA synthesis. Current transcriptome analysis approaches are limited to analysis of long (>200 nts) or short (~20 nts) RNAs. Here we describe an approach that can be used for the analysis of the medium-sized (~200 nts) RNA transcriptome, which reveals the full-length sequence of RNAs at the single molecule level. Our approach can easily be adapted to generate libraries that are suitable for sequencing by several of the currently used next-generation platforms.
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4. |
- Conze, Lei Liu, et al.
(författare)
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The primate spliceosomal snRNA transcriptome as revealed by deep sequencing
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Small nuclear RNAs (snRNAs) in combination with protein factors form the spliceosomes and are essential for pre-mRNA splicing. In this study we have characterized the spliceosomal snRNA (U snRNA) transcriptome in human, chimpanzee and rhesus using a recently developed high-throughput sequencing approach. Our study has revealed that the U snRNA transcriptomes of all three primates have a similar composition and that the vast majority of the U snRNAs originate from a few well-defined loci. We have detected some heterogeneity in the U snRNA transcriptomes that is primarily caused by the presence of SNPs in the few expressed loci that we have identified.
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