| 1. |
- Holmberg, Martin, et al.
(författare)
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Bacteria classification based on feature extraction from sensor data
- 1998
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Ingår i: Biotechnology Techniques. - : Kluwer Academic Publishers. - 0951-208X .- 1573-6784. ; 12:4, s. 319-324
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Tidskriftsartikel (refereegranskat)abstract
- Data evaluation and classification have been made on measurements by an electronic nose on the headspace of samples of different types of bacteria growing on petri dishes. The chosen groups were: Escherichia coli, Enterococcus sp., Proteus mirabilis, Pseudomonas aeruginosa, and Staphylococcus saprophytica. An approximation of the response curve by time was made and the parameters in the curve fit were taken as important features of the data set. A classification tree was used to extract the most important features. These features were then used in an artificial neural network for classification. Using the ‘leave-one-out’ method for validating the model, a classification rate of 76% was obtained
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| 2. |
- Na, A., et al.
(författare)
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Synthesis of phosphatidylcholine with (n-3) fatty acids by phospholipase A 2 in microemulsion
- 1990
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Ingår i: Journal of the American Oil Chemists Society. - 0003-021X .- 1558-9331. ; 67:11, s. 766-770
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Tidskriftsartikel (refereegranskat)abstract
- Phosphatidylcholine containing a long chain polyunsaturated acyl group at the 2-position has been prepared by phospholipase A 2 catalyzed esterification of lysophosphatidylcholine with polyunsaturated fatty acids EPA (C20:5) or DHA (C22:6). Preliminary studies showed that the other fatty acids, such as lauric acid (C12), palmitic acid (C16), stearic acid (C18) and linoleic acid (C18:2), were also incorporated. To our knowledge, phospholipase A 2 catalyzed condensation reactions have not been reported in the literature before. The reactions were performed in sodium bis(2-ethylhexyl)-sulfosuccinate-based microemulsions containing small amounts of water. Synthesis of the same phospholipid by transesterification of phosphatidylcholine with the polyunsaturated acids in microemulsion failed; however, enzymatic hydrolysis to lysophosphatidylcholine was facile, quantitative conversion from phosphatidylcholine being attained after 16 hr reaction time. An additional observation was that, unlike enzymatic hydrolysis of phospholipids, the condensation reaction catalyzed by phospholipase A 2 was totally independent of the presence of calcium. © 1990 AOCS Press.
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