| 1. |
- Imanishi, T., et al.
(författare)
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Integrative annotation of 21,037 human genes validated by full-length cDNA clones
- 2004
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Ingår i: PLoS biology. - : Public Library of Science (PLoS). - 1544-9173 .- 1545-7885. ; 2:6, s. 856-875
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Tidskriftsartikel (refereegranskat)abstract
- The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this area is still limited. Several lines of investigation have been used to elucidate the structure and function of the genes in the human genome. Even so, gene prediction remains a difficult task, as the varieties of transcripts of a gene may vary to a great extent. We thus performed an exhaustive integrative characterization of 41,118 full-length cDNAs that capture the gene transcripts as complete functional cassettes, providing an unequivocal report of structural and functional diversity at the gene level. Our international collaboration has validated 21,037 human gene candidates by analysis of high-quality full-length cDNA clones through curation using unified criteria. This led to the identification of 5,155 new gene candidates. It also manifested the most reliable way to control the quality of the cDNA clones. We have developed a human gene database, called the H-Invitational Database (H-InvDB; http://www.h-invitational.jp/). It provides the following: integrative annotation of human genes, description of gene structures, details of novel alternative splicing isoforms, non-protein-coding RNAs, functional domains, subcellular localizations, metabolic pathways, predictions of protein three-dimensional structure, mapping of known single nucleotide polymorphisms (SNPs), identification of polymorphic microsatellite repeats within human genes, and comparative results with mouse full-length cDNAs. The H-InvDB analysis has shown that up to 4% of the human genome sequence (National Center for Biotechnology Information build 34 assembly) may contain misassembled or missing regions. We found that 6.5% of the human gene candidates (1,377 loci) did not have a good protein-coding open reading frame, of which 296 loci are strong candidates for non-protein-coding RNA genes. In addition, among 72,027 uniquely mapped SNPs and insertions/deletions localized within human genes, 13,215 nonsynonymous SNPs, 315 nonsense SNPs, and 452 indels occurred in coding regions. Together with 25 polymorphic microsatellite repeats present in coding regions, they may alter protein structure, causing phenotypic effects or resulting in disease. The H-InvDB platform represents a substantial contribution to resources needed for the exploration of human biology and pathology.
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| 2. |
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| 3. |
- Fahlander, Claes, et al.
(författare)
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Excited States in 103Sn: Neutron Single-particle Energies with Respect to 100Sn
- 2001
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Ingår i: Physical Review C (Nuclear Physics). - 0556-2813 .- 1089-490X. ; 63:2
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Tidskriftsartikel (refereegranskat)abstract
- Gamma-ray lines from Sn-103 have been identified for the first time using EUROBALL and ancillary detectors. The level scheme of Sn-103 has been established by means of particle-gated gamma gamma coincidences. The energy spacing between the g(7/2) and d(5/2) neutron single-particle orbitals is determined from the excited states in Sn-103.
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| 4. |
- Palacz, M., et al.
(författare)
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Investigations of Neutron Deficient Nuclei Close to 100Sn with EUROBALL
- 2001
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Ingår i: Acta Physica Polonica. Series B: Elementary Particle Physics, Nuclear Physics, Statistical Physics, Theory of Relativity, Field Theory. - 0587-4254 .- 1509-5770. ; 32, s. 999-1003
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Tidskriftsartikel (refereegranskat)abstract
- Excited states of nuclei in the vicinity of Sn-100 have been studied using: the EUROBALL detector array. Gamma-ray lines from Sn-103 have been identified for the first time, and a level scheme of low-lying excited states of Sn-103 has been established. New constraints on energies of single particle orbitals with respect to the Sn-100 core are obtained.
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| 5. |
- Örnek, Cem, et al.
(författare)
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Characterization of 475 degrees C Embrittlement of Duplex Stainless Steel Microstructure via Scanning Kelvin Probe Force Microscopy and Magnetic Force Microscopy
- 2017
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Ingår i: Journal of the Electrochemical Society. - : Electrochemical Society. - 0013-4651 .- 1945-7111. ; 164:6, s. C207-C217
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Tidskriftsartikel (refereegranskat)abstract
- Scanning Kelvin probe force microscopy (SKPFM) measured local Volta potentials in microstructure of 22Cr-5Ni duplex stainless steel have been correlated to microstructure development with aging treatments at 475 degrees C. Magnetic force microscopy (MFM) was employed to differentiate crystallographic phases to provide complementary information. The absolute Volta potentials of both ferrite and austenite increased after 5 hours of aging, indicating electrochemical ennoblement of the entire microstructure. Longer aging resulted in a gradual decrease of measured Volta potentials in both phases. The microstructure showed after 255 hours aging up to 2.5-times larger potential differences than in the as-received condition, indicating impaired electrochemical nobility. In the as-received microstructure, the ferrite phase was less noble than the austenite, whereas after 5 hours aging both phases had similar, balanced Volta potentials which indicated a balanced nobility of ferrite and austenite. Longer aging treatment caused severe loss of nobility for the entiremicrostructure, with ferrite showing larger changes in Volta potential than the austenite. Spinodal microstructure decomposition and associated phase reactions of the ferrite, with elemental redistribution in the austenite, are the reason for the observed changes in microstructure nobility.
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| 6. |
- Hashimoto, K., et al.
(författare)
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A Symbolic Approach to the Self-Triggered Design for Networked Control Systems
- 2019
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Ingår i: IEEE Control Systems Letters. - : Institute of Electrical and Electronics Engineers (IEEE). - 2475-1456. ; 3:4, s. 1050-1055
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Tidskriftsartikel (refereegranskat)abstract
- In this letter, we investigate novel self-triggered controllers for nonlinear control systems with reachability and safety specifications. To synthesize the self-triggered controller, we leverage the notion of symbolic models, or abstractions, which represent abstracted expressions of control systems. The symbolic models will be constructed through the concepts of approximate alternating simulation relations, based on which, and by employing a reachability game, the self-triggered controller is synthesized. We illustrate the effectiveness of the proposed approach through numerical simulations.
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| 7. |
- Örnek, Cem, et al.
(författare)
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475 degrees C Embrittlement of Duplex Stainless Steel-A Comprehensive Microstructure Characterization Study
- 2017
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Ingår i: Materials Performance and Characterization. - : American society for testing and materials. - 2379-1365 .- 2165-3992. ; 6:3, s. 409-436
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Tidskriftsartikel (refereegranskat)abstract
- The effect of 475 degrees C embrittlement on microstructure development of grade 2205 duplex stainless steel was investigated. Spinodal decomposition products and associated precipitates in ferrite, austenite, and at interphase boundaries were characterized using analytical transmission electron microscopy (TEM) and scanning electron microscopy (SEM) techniques. Microanalyses confirmed the presence of Cr-enriched alpha'' and Cr-depleted alpha' spinodal structures in the ferrite after 5 h of aging at 475 degrees C. Long-term aging for 255 h resulted in heavily-faulted R-phase precipitates with sizes of similar to 50-400 nm, chi-phase, and epsilon-Cu in the ferrite, TiN and Cr2N precipitates in the austenite, and a continuous network of M23C6-carbides at interphase boundaries. A significant hardness increase was observed after 255 h of aging, which was accompanied by a reduction of ferrite fraction. X-ray diffraction (XRD) stress measurements showed a general reduction of residual stresses in both ferrite and austenite with aging. Electron backscatter diffraction (EBSD) showed increased local misorientations, primarily close to precipitate interfaces within the ferrite, indicating the development of strain heterogeneities in the microstructure. The data presented provided a better understanding of 475 degrees C embrittlement in duplex stainless steel, suggesting that not only the ferrite alone is responsible for embrittlement. A comprehensive microstructure characterization study has been provided and the explanation for 475 degrees C embrittlement of duplex stainless steel has been discussed.
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| 8. |
- Örnek, Cem, et al.
(författare)
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748 K (475 A degrees C) Embrittlement of Duplex Stainless Steel : Effect on Microstructure and Fracture Behavior
- 2017
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Ingår i: Metallurgical and Materials Transactions. A. - : SPRINGER. - 1073-5623 .- 1543-1940. ; 48A:4, s. 1653-1665
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Tidskriftsartikel (refereegranskat)abstract
- 22Cr-5Ni duplex stainless steel (DSS) was aged at 748 K (475 A degrees C) and the microstructure development correlated to changes in mechanical properties and fracture behavior. Tensile testing of aged microstructures confirmed the occurrence of 748 K (475 A degrees C) embrittlement, which was accompanied by an increase of strength and hardness and loss of toughness. Aging caused spinodal decomposition of the ferrite phase, consisting of Cr-enriched alpha aEuro(3) and Fe-rich alpha' and the formation of a large number of R-phase precipitates, with sizes between 50 and 400 nm. Fracture surface analyses revealed a gradual change of the fracture mode from ductile to brittle delamination fracture, associated with slip incompatibility between ferrite and austenite. Ferrite became highly brittle after 255 hours of aging, mainly due to the presence of precipitates, while austenite was ductile and accommodated most plastic strain. The fracture mechanism as a function of 748 K (475 A degrees C) embrittlement is discussed in light of microstructure development.
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