| 1. |
- KRISTOFFERSSON, ULF, et al.
(författare)
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CYTOGENETIC STUDIES IN HODGKIN'S DISEASE
- 1987
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Ingår i: Acta Pathologica Microbiologica Scandinavica. Section A. Pathology. - 0108-0164. ; 95 A:1-6, s. 289-295
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Tidskriftsartikel (refereegranskat)abstract
- Cytogenetic analysis was attempted in 20 patients with Hodgkin's disease. No mitoses were found in 2 cases, normal metaphases in 7, and normal metaphases with nonclonal aberrations in 7. Of the 4 cases with clonal aberrations, one had +16 as the sole change, whereas the remaining tumors had multiple numerical and structural changes.
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| 2. |
- KRISTOFFERSSON, ULF, et al.
(författare)
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Cytogenetic studies in non‐Hodgkin lymphomas ‐ Results from surgical biopsies
- 1986
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Ingår i: Hereditas. - : Springer Science and Business Media LLC. - 0018-0661. ; 104:1, s. 1-13
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Tidskriftsartikel (refereegranskat)abstract
- Cytogenetic analysis using Giemsa banding technique was successful in 30 of the 49 adult patients with nonHodgkin lymphomas investigated. The results are correlated with previous findings in 48 non‐Hodgkin lymphoma patients studied by means of fine needle aspiration in our laboratory. As 8 patients were included in both series, the total number successfully investigated was 70. The success rate with both sampling techniques was equivalent, and both methods also seemed to give qualitatively similar information about chromosome pattern. However, cells with a normal diploid karyotype were more frequent in the surgical biopsies. In the total material, normal karyotypes only were found in 10 patients. In two patients the aberrations were too complex to allow evaluation. The chromosome variation among the remaining 58 cases was distinctly nonrandom. Chromosomes 3, 7, 12, and 18 were preferentially gained, whereas chromosomes 1, 6, and 14 were most often involved in structural rearrangements. A 14q+ marker chromosome was the single most frequent abnormality; originating through t(14; 18) (q32; q21) in 10 of 23 cases. The second most common structural aberration was a deletion of the long arm of chromosome 6 with breakpoints at bands q15 and q21 (12 cases). At least one of the two most common numerical and/or structural changes, +7, +12, 14q+, and 6q‐, were present in 39 of the 58 patients with aberrations (67%). Longitudinal studies demonstrated karyotypic evolution during the course of the disease in five of six patients. Simultaneous samples from different tumor sites were studied in 10 patients. The findings in 9 cases suggested a monoclonal origin; in one case totally unrelated karyotypes were found in two different lymph nodes at third relapse, suggesting a multifocal origin.
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| 3. |
- Kristoffersson, Ulf, et al.
(författare)
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Deletion of 14q in non‐Hodgkin's lymphoma
- 1990
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Ingår i: European Journal of Haematology. - : Wiley. - 0902-4441 .- 1600-0609. ; 44:4, s. 261-264
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Tidskriftsartikel (refereegranskat)abstract
- Abstract: 6 patients with non‐Hodgkin's lymphoma [3 with small cell lymphocytic lymphoma of B‐cell type (SL), and 1 each with follicular centroblastic/centrocytic, centroblastic, and immunoblastic lymphoma] and with the acquired cytogenetic abnormalities del(14) (q22) or del(14) (q24) are described. An evaluation of these 6 cases and 41 other lymphatic neoplasms with 14q deletion known from the literature revealed that 37 had a breakpoint in bands q22 to q24. The deletions occur significantly more often in lymphomas of SL morphology and in the leukemic counterpart, chronic lymphocytic leukemia, than in other types of lymphatic malignancies (p< 0.001).
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| 4. |
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| 5. |
- Kristoffersson, Ulf, et al.
(författare)
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Prognostic implication of cytogenetic findings in 106 patients with non-Hodgkin lymphoma
- 1987
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Ingår i: Cancer Genetics and Cytogenetics. - : Elsevier BV. - 0165-4608. ; 25:1, s. 55-64
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Tidskriftsartikel (refereegranskat)abstract
- The cytogenetic findings in samples from 106 patients with non-Hodgkin lymphomas (NHL), histopathologically classified according to the Kiel classification, have been correlated with survival time. Clonal chromosomal abnormalities were found in 60 patients, and only normal karyotypes in ten. The chromosome analysis of the remaining samples failed. The failures did not differ in survival compared with the cytogenetically successful cases, indicating that this group is not a prognostic entity within NHL. The cytogenetic findings were classified in six ways in order to evaluate the prognostic value of the cytogenetic pattern. Multivariate analysis demonstrated that presence of clonal chromosome abnormalities and the number of aberrations both were important prognostic factors independent of histopathology, whereas, the modal chromosome number, presence of translocations, or unidentified marker chromosomes were not. Some characteristic chromosome abnormalities were correlated with survival time: Patients with a 1p+ marker or +7 had a significantly shorter survival time than patients with normal karyotypes only (NN). Patients with +3, +12, 6q-, i(17q), and t(14;18)(q32;q21) did not differ significantly from the NN group.
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| 6. |
- KRISTOFFERSSON, ULF, et al.
(författare)
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RELATIONSHIP BETWEEN CYTOGENETIC FINDINGS AND HISTOPATHOLOGY IN NON‐HODGKIN LYMPHOMA
- 1987
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Ingår i: Acta Pathologica Microbiologica Scandinavica. Section A. Pathology. - 0108-0164. ; 95 A:1-6, s. 1-5
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Tidskriftsartikel (refereegranskat)abstract
- The cytogenetic findings in 70 patients with non‐Hodgkin lymphoma have been correlated with tumor histopathology according to the Kiel classification. Certain chromosome aberrations displayed a nonrandom association with the grade of malignancy: 4 lymphomas out of 6 with 1p+, 5 out of 7 with del(6)(q15), 7 out of 11 with 14q+, and 5 out of 8 with +18 belonged to the high grade malignancy group, whereas 9 lymphomas out of 10 with t(14;18) were low grade malignant. Two aberration types were closely associated with specific histopathologic subtypes: t(14; 18) occurred in 7 cases out of 10 in centroblastic/ centrocytic (cb/cc) follicular lymphomas, and 5 cases out of 6 with i(17q) were cb or cb/cc. Although less striking, there was a tendency for del(6)(q15) to occur in cb or cb/cc lymphomas (4 cases out of 7), in contrast to only 1 case out of 5 with the more distal deletion del(6)(q21).
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| 7. |
- Kristoffersson, Ulf, et al.
(författare)
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Trisomy 5 and t(5;14)(q11;q32) as the sole abnormalities in two different clones from a centroblastic non-Hodgkin's lymphoma
- 1988
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Ingår i: Cancer Genetics and Cytogenetics. - : Elsevier BV. - 0165-4608. ; 36:2, s. 173-176
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Tidskriftsartikel (refereegranskat)abstract
- A 62-year-old previously healthy woman presented with a centroblastic non-Hodgkin's lymphoma in the thyroid. Chromosome analysis revealed two unrelated clones, 47,XX,+5 and 46,XX,-14,+der(14)t(5;14)(q11;q32). The two clones may reflect a polyclonal origin, or they may be the descendants of the same neoplastically rearranged cell. In the latter case, the clonal aberrations are either secondary to an event detectable only at the molecular level, or one of them is a primary cytogenetic event while the other arose through clonal evolution with loss of the primary aberration. The best candidate for the primary change would be trisomy 5. Trisomy 5 has previously been associated with lymphomas with diffuse, large, noncleaved morphology, a group within the Working Formulation largely equivalent to centroblastic lymphomas in the Kiel classification. Our findings thus support the notion that trisomy 5 may be associated with centroblastic/diffuse, large, noncleaved lymphomas.
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| 8. |
- Mandahl, Nils, et al.
(författare)
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Comparative cytogenetic and DNA flow cytometric analysis of 150 bone and soft-tissue tumors
- 1993
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Ingår i: International Journal of Cancer. - : Wiley. - 0020-7136 .- 1097-0215. ; 53:3, s. 358-364
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Tidskriftsartikel (refereegranskat)abstract
- Samples from 48 benign and 102 malignant bone and soft-tissue tumors were analyzed cytogenetically and by DNA flow cytometry. Clonal chromosome abnormalities were found in 82 tumors and normal karyotypes in 68; 61 tumors were DNA-non-diploid and 89 were diploid. The cytogenetically abnormal tumors were used for comparison between the 2 types of investigation; 45 of these tumors were DNA-diploid and 37 were DNA-non-diploid. There was, with few exceptions, good correspondence between the quantitative estimates of genomic changes by the 2 methods, indicating that the cells cytogenetically analyzed from short-term cultures are representative of the in vivo cell populations. Discrepancies were primarily found in cases with indexes above 1.5, in which the DNA index was higher than the chromosome index. The chromosome analysis suggested that skewed stemline (G0/G1) peaks in the diploid region in DNA histograms indicate the presence of cell populations with small net quantitative genomic changes, although not all such populations were detected by DNA flow cytometric analysis. The view that one of the peaks in bimodal stemline DNA histograms with narrow peaks represents a non-diploid cell population was also corroborated. On average, the cell populations giving rise to double stemlines in DNA histograms showed quantitatively larger genomic changes than those that gave rise to broad or skewed diploid G0/G1 peaks. The findings indicate that these histogram profiles are not artifactual but reflect chromosomal changes in the tumor parenchyma.
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