| 1. |
- Dahlgren, Liselotte, et al.
(författare)
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Differences in human papillomavirus type may influence clinical outcome in early stage cervical cancer.
- 2006
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Ingår i: Anticancer Research. - 0250-7005 .- 1791-7530. ; 26:2A, s. 829-32
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: The presence of human papillomavirus (HPV), the HPV type and viral load in early stage cervical carcinoma were investigated in order to elucidate whether any of these factors were important for clinical outcome. PATIENTS AND METHODS: Twelve patients who were disease-free 5 years after diagnosis were matched and compared with 12 patients who died within 2 years. The presence of HPV, HPV type and viral load in their tumours was examined by PCR. RESULTS: The distribution and load of HPV was similar in the 2 patient groups. HPV-16 was, however, significantly more common in tumours of the surviving patients than in those of patients who died (88.9% and 18.2%, respectively, p = 0.0152). CONCLUSION: HPV-16 was significantly more common in early stage carcinomas of patients surviving more than 5 years in comparison to early stage carcinomas of patients with a poor prognosis.
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| 2. |
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| 3. |
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| 4. |
- Hellman, Kristina, et al.
(författare)
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Differential tissue-specific protein markers of vaginal carcinoma
- 2009
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Ingår i: British Journal of Cancer. - : Cancer Research UK. - 0007-0920 .- 1532-1827. ; 100:8, s. 1303-1314
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Forskningsöversikt (refereegranskat)abstract
- The objective was to identify proteins differentially expressed in vaginal cancer to elucidate relevant cancer-related proteins. A total of 16 fresh-frozen tissue biopsies, consisting of 5 biopsies from normal vaginal epithelium, 6 from primary vaginal carcinomas and 5 from primary cervical carcinomas, were analysed using two-dimensional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry. Of the 43 proteins identified with significant alterations in protein expression between non-tumourous and tumourous tissue, 26 were upregulated and 17 were downregulated. Some were similarly altered in vaginal and cervical carcinoma, including cytoskeletal proteins, tumour suppressor proteins, oncoproteins implicated in apoptosis and proteins in the ubiquitin-proteasome pathway. Three proteins were uniquely altered in vaginal carcinoma (DDX48, erbB3-binding protein and biliverdin reductase) and five in cervical carcinoma (peroxiredoxin 2, annexin A2, sarcomeric tropomyosin kappa, human ribonuclease inhibitor and prolyl-4-hydrolase beta). The identified proteins imply involvement of multiple different cellular pathways in the carcinogenesis of vaginal carcinoma. Similar protein alterations were found between vaginal and cervical carcinoma suggesting common tumourigenesis. However, the expression level of some of these proteins markedly differs among the three tissue specimens indicating that they might be useful molecular markers.
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| 5. |
- Hellström, Ann-Cathrin
(författare)
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Primary fallopian tube cancer
- 1997
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Primary fallopian tube cancer (PFTC) is a rare and aggressive gynecologic malignancy and it accounts for less than 5% of all genital female cancers according to the Swedish Cancer Registry. In a retrospective study we have analysed histopathological, clinical, biological and prognostic factors among patients with PFTC. 128 cases of PFTC treated between 1923 and 1991 were histopathologically reviewed and retrospectively staged according to the new FIGO staging system. Seventy-four percent were found to be instage Ia-IIa, compared to 36% in stage Ia-IIa among ovarian cancer cases treated at Radiumhemmet in 1958 to 1973. In stage Ia a 5-year actuarial survival of 70.1% was demonstrated. Forty-five percent were nulliparous and the mean age at diagnosis was 56 years. Twenty-two per cent were suffering from salpingitis and the infertility rate was 25%. Among the 14 variables studied in the clinical and pathological review and tested in the multivariate analysis the first in rank were the stage (p=0.001) and grade of differentiation of the tumors (p=0.070). Treatment modalities changed during the studied period. Thirty-three per cent of patients underwent surgery with total abdominal hysterectomy and bilateral salpingo oophorectomy. Patients receiving chemotherapy had superior survival rates (p=0.0006) and patients with cisplatinum-containing chemotherapy did better than those without. In this thesis, attempts have been made to further characterize the malignancy potential of the PFTC by biological prognostic factors. The patients in the maternal were divided into two groups according to survival time and the interval was chosen in order to get a maximal contrast with regard to survival at a minimal cost - short-time survivors < 2years and long-time survivors > 8 years. We have evaluated the DNA ploidy content, proliferative activity (MIB-I), p53 overexpression combined or not with the downstream product p21/WAF-I, and tumor angiogenesis (F8) in correlation to clinical outcome. Furthermore, we studied the frequences of p53 mutations in PFTC and in their corresponding metastases/recurrences as well as the influence of p53 on therapeutic effect and clinical course in individual cases with careful clinical follow-up. All PFTCs showed aneuploid DNA distribution patterns. The following investigated biological markers (DNA, MIB-I, P53, p21/WAF-I, F8) could not discriminate between short- and long-time survivors. A tendency to increased frequency of mutations (combined p53 and p21/WAF-I) was found among short-time survivors in stage I as well as in stage IV. Increasing MIB-I correlated to poor survival in a multivariate analysis of stage I cases. MMMT showed aneuploid DNA content patterns. MIB-I, p53, WAF-I, and F8did not allow discrimination between short- and long-time survivors. A comparison of SSCP and CDGE suggests that CDGE is superior as a TP53 gene mutation detection method. In spite of the limited number of cases investigated, our data indicate that p53 immunoreactivity and TP53 mutation analysis is not correlated to tumor progression, survival and response to treatment. CGH analysis indicates that PFTC is a genomically highly unstable type of malignancy and 3 q gain/ amplification is the major event. The frequent 3q-gains observed in HPV infected cervical carcinoma do not appear to be related to the insertion of virus DNA since all PFTC cases studied were found to be exclusively HPV negative. In conclusion, this study has confirmed that PFTC is an aggressive gynecologic malignancy which showed a high number of chromosomal aberrations comprising all chromosomes and without connection to HPV infection. This is in agreement with the high frequency (100%) of DNA aneuploid cases. None of the biological markers investigated was found to be of principal value in predicting the clinical outcome of PFTC. Only the clinical stage was found to be significantly related to patient survival.
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| 6. |
- Lomnytska, Marta I, et al.
(författare)
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Diagnostic protein marker patterns in squamous cervicalcancer
- 2010
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Ingår i: Proteomics - Clinical Applications. - Weinheim : WILEY-VCH Verlag GmbH & Co. KGaA. - 1862-8346 .- 1862-8354. ; 4:1, s. 17-31
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: Cervical cancer is the second most prevalent malignancy of women. Our aim was toidentify additional marker protein patterns for objective diagnosis of squamous cervical cancer(SCC).Experimental design: Collected tissue biopsies of SCC, squamous vaginal cancer (SVC), normalcervical and vaginal mucosa were subjected to 2-DE, SameSpot analysis, MALDI-TOF-MSprotein identification, and analysis of the expression of selected proteins by immunohistochemistry.Results: In 148 protein spots selected by the difference in expression 99 proteins were identified.A differential protein pattern for SCC was, e.g. over-expressed (OE) eukaryotic translationinitiation factor 3-2b, neutrophil cytosolic factor 2, annexin A6 (ANXA6), for SVC it was OEcathepsin D, g-catenin, RAB2A, for both cancers it was OE apolipoprotein E, tropomyosin 3,HSPA8, and underexpressed cytokeratin 13, osteoglycin. In SCC nuclear expression ofneutrophil cytosolic factor 2, PRDX2, HSP27 (nine of ten cases), ANXA6 (nine of ten cases) wasobserved while tropomyosin 4 was expressed only in two of ten cases. There was 81.1% (43/53)agreement between the expression of protein spots and the immune expression of proteins(www.proteinatlas.org).Conclusions and clinical relevance: SCC is characterized by specific tissue marker proteinpatterns that allow objective detection of the disease. They can become a basis for objectiveautomated cytology-based screening and improve current diagnostics of SCC.
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| 7. |
- Ranhem, Cecilia, et al.
(författare)
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Expression of LRIG proteins as possible prognostic factors in primary vaginal carcinoma
- 2017
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Ingår i: PLOS ONE. - : Public Library of Science. - 1932-6203. ; 12:8
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Tidskriftsartikel (refereegranskat)abstract
- Background: Primary vaginal carcinoma (PVC) is a rare malignancy. Established prognostic factors include tumour stage and age at diagnosis. The leucine-rich repeats and immunoglobuline-like domains (LRIG)-1 protein functions as a tumour suppressor, but less is known about the functions of LRIG2 and LRIG3. The present study aimed to evaluate the expression of LRIG proteins and analyse their possible associations with clinical characteristics and survival in a cohort of PVC patients.Methods: We used immunohistochemistry to investigate LRIG1, LRIG2, and LRIG3 expression in tumour samples from a consecutive cohort of 70 PVC patients. The association between LRIG protein expression and clinical characteristics and cancer-specific survival was investigated using univariate and multivariate analyses.Results: The majority of PVC patients (72%) had > 50% LRIG1-and LRIG2-positive cells, and no or low LRIG3-positive cells. HPV status was significantly correlated with LRIG1 expression (p = 0.0047). Having high LRIG1 expression was significantly correlated with superior cancer-specific survival in univariate and multivariate analyses. LRIG2 and LRIG3 expression did not significantly correlate with clinical characteristics or survival.Conclusion: LRIG1 expression might be of interest as a prognostic marker in PVC patients, whereas the role of LRIG2 and LRIG3 expression remains to be clarified.
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| 8. |
- Tuvemo Johnson, Susanna, et al.
(författare)
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Older adults' opinions on fall prevention in relation to physical activity level
- 2018
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Ingår i: Journal of Applied Gerontology. - : SAGE Publications. - 0733-4648 .- 1552-4523. ; 37:1, s. 58-78
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Tidskriftsartikel (refereegranskat)abstract
- The purpose of this study was to explore and describe older adults' opinions regarding actions to prevent falls and to analyze differences in the opinions of highly versus less physically active older adults. An open-ended question was answered by 262 individuals aged 75 to 98 years living in the community. The answers were analyzed using qualitative content analysis, and differences in the categories were compared between highly and less physically active persons. Physical activity was measured according to a five-level scale. The content analysis resulted in eight categories: assistive devices, avoiding hazards, behavioral adaptive strategies, being physically active, healthy lifestyle, indoor modifications, outdoor modifications, and seeking assistance. Behavioral adaptive strategies were mentioned to a greater extent by highly active people, and indoor modifications were more often mentioned by less active older adults. Support for active self-directed behavioral strategies might be important for fall prevention among less physically active older adults.
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| 9. |
- von Haartman, Martin, et al.
(författare)
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1/f noise in Si and Si0.7Ge0.3 pMOSFETs
- 2003
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Ingår i: IEEE Transactions on Electron Devices. - 0018-9383 .- 1557-9646. ; 50, s. 2513-2519
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Tidskriftsartikel (refereegranskat)abstract
- Strained layer Si0.7Ge0.3 pMOSFETs were fabricated and shown to exhibit enhanced hole mobility, up to 35% higher for a SiGe device with 3-nm-thick Si-cap, and lower 1/f noise compared to Si surface channel pMOSFETs. The 1/f noise in the investigated devices was dominated by mobility fluctuation noise and found to be lower in the SiGe devices. The source of the mobility fluctuations was determined by investigating the electric field dependence of the 1/f noise. It was found that the SiO2/Si interface roughness scattering plays an important role for the mobility fluctuation noise, although not dominating the effective mobility. The physical separation of the carriers from the SiO2/Si interface in the buried SiGe channel pMOSFETs resulted in lower SiO2/Si interface roughness scattering, which explains the reduction of 1/f noise in these devices. The 1/f noise mechanism was experimentally verified by studying 1/f noise in SiGe devices with various thicknesses of the Si-cap. A too large Si-cap thickness led to a deteriorated carrier confinement in the SiGe channel resulting in that considerable 1/f noise was generated in the parasitic current in the Si-cap. In our experiments, the SiGe devices with a Si-cap thickness in the middle of the interval 3-7 nm exhibited the lowest 1/f noise.
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| 10. |
- von Haartman, Martin, et al.
(författare)
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Influence of gate width on 50 nm gate length Si0.7Ge0.3 channel PMOSFETs
- 2003
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Ingår i: ESSDERC 2003: PROCEEDINGS OF THE 33RD EUROPEAN SOLID-STATE DEVICE RESEARCH CONFERENCE. ; , s. 529-532
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Konferensbidrag (refereegranskat)abstract
- Compressively strained Si0.7Ge0.3 channel pMOSFETs were fabricated and the effective hole mobility was found to be 20-30% higher in the Si0.7Ge0.3 devices than in their Si counterparts. The g(m,) normalized to gate width, was found to increase strongly with decreasing gate width in the Si0.7Ge0.3 devices, a behavior that was not found in the Si devices. All the Si0.7Ge0.3 devices down to 50 nm gate length showed enhanced g. compared to the Si devices for gate widths <1 um. At L = 50 nm and W = 0.25 mum the Si0.7Ge0.3 devices exhibited increased g(m) and I-D of about 15 %, in saturation, compared to the Si devices. I-on was 286 muA/mum and I-off was 0.23 nA/mum at V-dd = 1.5 Vfor the Si0.7Ge0.3 device.
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