| 1. |
- Carmona-Gutierrez, D., et al.
(författare)
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Guidelines and recommendations on yeast cell death nomenclature
- 2018
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Ingår i: Microbial Cell. - : Shared Science Publishers OG. - 2311-2638. ; 5:1, s. 4-31
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Forskningsöversikt (refereegranskat)abstract
- Elucidating the biology of yeast in its full complexity has major implications for science, medicine and industry. One of the most critical processes determining yeast life and physiology is cellular demise. However, the investigation of yeast cell death is a relatively young field, and a widely accepted set of concepts and terms is still missing. Here, we propose unified criteria for the definition of accidental, regulated, and programmed forms of cell death in yeast based on a series of morphological and biochemical criteria. Specifically, we provide consensus guidelines on the differential definition of terms including apoptosis, regulated necrosis, and autophagic cell death, as we refer to additional cell death routines that are relevant for the biology of (at least some species of) yeast. As this area of investigation advances rapidly, changes and extensions to this set of recommendations will be implemented in the years to come. Nonetheless, we strongly encourage the authors, reviewers and editors of scientific articles to adopt these collective standards in order to establish an accurate framework for yeast cell death research and, ultimately, to accelerate the progress of this vibrant field of research.
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| 2. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 3. |
- Hofer, J., et al.
(författare)
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High- Q Magnetic Levitation and Control of Superconducting Microspheres at Millikelvin Temperatures
- 2023
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Ingår i: Physical Review Letters. - 1079-7114 .- 0031-9007. ; 131:4
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Tidskriftsartikel (refereegranskat)abstract
- We report the levitation of a superconducting lead-tin sphere with 100 μm diameter (corresponding to a mass of 5.6 μg) in a static magnetic trap formed by two coils in an anti-Helmholtz configuration, with adjustable resonance frequencies up to 240 Hz. The center-of-mass motion of the sphere is monitored magnetically using a dc superconducting quantum interference device as well as optically and exhibits quality factors of up to 2.6×107. We also demonstrate 3D magnetic feedback control of the motion of the sphere. The setup is housed in a dilution refrigerator operating at 15 mK. By implementing a cryogenic vibration isolation system, we can attenuate environmental vibrations at 200 Hz by approximately 7 orders of magnitude. The combination of low temperature, large mass, and high quality factor provides a promising platform for testing quantum physics in previously unexplored regimes with high mass and long coherence times.
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| 4. |
- Malakhatka, Elena, 1989, et al.
(författare)
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Towards Positive Energy Districts: Multi-criteria framework and Quality Assurance
- 2024
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Ingår i: IOP Conference Series: Earth and Environmental Science. - 1755-1307 .- 1755-1315. ; 1363:1
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Konferensbidrag (refereegranskat)abstract
- This research develops a multi-criteria framework and Quality Assurance checklist (QA) for Positive-Energy-Districts (PEDs). PEDs have a large potential to contribute to reach urban climate goals but require also changes of planning, development, and transformation processes of neighborhoods. Our understanding of PEDs has been broadened to include aspects of circularity and economic viability, ensuring that PED concepts not only contribute to environmental sustainability but are also economically feasible. In this enhanced perspective, Digital Twins serving as essential carriers of information and facilitators of communication, effectively bridging the gap between different dimensions of sustainable development and practical implementation and monitoring. This study is part of an ongoing European project, Digital Twin for PEDs (DT4PEDs), with Living Labs in three countries: Austria, Sweden, and Turkey. The presented study addresses the central research question, "In what phases can specific requirements be linked to the process to ensure PED development throughout the entire process using a Digital Twin?" within the context of the DT4PEDs project. Based on a participatory workshop with stakeholders from partner countries and a parallel literature review, the study encompasses three key areas. First, it seeks to consolidate property development practices across Austria, Sweden, and Turkey, including both new construction and retrofitting, into a unified framework. Second, it formulates a PED multi-criteria framework, complemented by a Quality Assurance (QA) checklist. Finally, the recommendations for digital twin are proposed to support energy related information flows and stakeholder dialogue.
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| 5. |
- Schmidt, P., et al.
(författare)
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Remote sensing of a levitated superconductor with a flux-tunable microwave cavity
- 2024
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Ingår i: Physical Review Applied. - 2331-7019. ; 22:1
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Tidskriftsartikel (refereegranskat)abstract
- We present a cavity-electromechanical system comprising a superconducting quantum interference device which is embedded in a microwave resonator and coupled via a pickup loop to a 6-μg magnetically levitated superconducting sphere. The motion of the sphere in the magnetic trap induces a frequency shift in the SQUID-cavity system. We use microwave spectroscopy to characterize the system, and we demonstrate that the electromechanical interaction is tunable. The measured displacement sensitivity of 10-7m/Hz defines a path towards ground-state cooling of levitated particles with Planck-scale masses at millikelvin environment temperatures.
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| 6. |
- Schmitt, Andreas, et al.
(författare)
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PrgB promotes aggregation, biofilm formation, and conjugation through DNA binding and compaction
- 2018
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Ingår i: Molecular Microbiology. - : Wiley. - 1365-2958 .- 0950-382X. ; 109:3, s. 291-305
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Tidskriftsartikel (refereegranskat)abstract
- Gram-positive bacteria deploy type IV secretion systems (T4SSs) to facilitate horizontal gene transfer. The T4SSs of Gram-positive bacteria rely on surface adhesins as opposed to conjugative pili to facilitate mating. Enterococcus faecalis PrgB is a surface adhesin that promotes mating pair formation and robust biofilm development in an extracellular DNA (eDNA) dependent manner. Here, we report the structure of the adhesin domain of PrgB. The adhesin domain binds and compacts DNA in vitro. In vivo PrgB deleted of its adhesin domain does not support cellular aggregation, biofilm development and conjugative DNA transfer. PrgB also binds lipoteichoic acid (LTA), which competes with DNA binding. We propose that PrgB binding and compaction of eDNA facilitates cell aggregation and plays an important role in establishment of early biofilms in mono- or polyspecies settings. Within these biofilms, PrgB mediates formation and stabilization of direct cell-cell contacts through alternative binding of cell-bound LTA, which in turn promotes establishment of productive mating junctions and efficient intra- or inter-species T4SS-mediated gene transfer.
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