| 1. |
|
|
| 2. |
- Gjörloff Wingren, Anette, et al.
(författare)
-
In vitro effects of undifferentiated callus extracts from Plantago major L, Rhodiola rosea L and Silybum marianum L in normal and malignant human skin cells cells
- 2023
-
Ingår i: Heliyon. - : Elsevier. - 2405-8440. ; 9:6
-
Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND AND OBJECTIVES: L was investigated both in normal and malignant skin cells.METHODS: Antioxidant activity of the extracts was analyzed by using the Trolox Equivalent Antioxidant Capacity (TEAC) assay. High-Performance Thin-Layer Chromatography (HPTLC) was performed to demonstrate the phytochemical profile, and the total flavonoid content was analyzed with an aluminum chloride colorimetric method. The anti-inflammatory effect was investigated by cell treatments using the plant extracts. Thereafter, the possible suppression of induced IL-6 response was measured from the cultured skin cancer cell lines A2058 and A431, and normal primary keratinocytes with Enzyme-Linked Immunosorbent Assay (ELISA).RESULTS: also had the highest flavonoid content and showed the highest antioxidant activity of the three extracts tested.CONCLUSION: possess properties such as antioxidant and anti-inflammatory activities in both normal and malignant keratinocytes, and thus could be a promising agent controlling the pro-inflammatory IL-6 production.
|
|
| 3. |
- Gustavsson, Larisa, et al.
(författare)
-
Screening of Apple Cultivars for Resistance to European Canker, Neonectria ditissima
- 2013
-
Ingår i: Acta Horticulturae. - 0567-7572 .- 2406-6168. ; 976, s. 529-536
-
Konferensbidrag (refereegranskat)abstract
- European canker, caused by the fungus Neonectria ditissima, is a severe problem in apple production both in Sweden and in many other northern European countries. Even when applying fungicides and good horticultural practices, canker damage occurs almost yearly in nurseries and orchards. Some years, devastating outbreaks destroy numerous trees. To date, complete resistance to N. ditissima is not known in apple. For further research and plant breeding, heritable variation in quantitative resistance should be investigated by phenotyping large sets of cultivars. In the present project, 55 apple cultivars were screened for resistance to N. ditissima. One-year-old shoots from mature trees were inoculated in the greenhouse with a standardized volume and concentration of conidia suspension using different inoculation methods. Two-year-old trees of five cultivars were inoculated in the field. Length of the occurring cankers was measured at regular intervals throughout a period of up to three months. The investigated cultivars showed considerable differences in colonization rate. In cultivars known to be highly resistant, i.e., 'Santana', lesions progressed much slower compared to susceptible cultivars like 'Cox's Orange Pippin' and 'James Grieve'. Since the inoculation-based phenotyping is demanding in labour and time (duration), especially when the test is performed on grafted trees, qPCR-based assessment of fungal biomass at early stages of infection was explored as an alternative or complementary approach for phenotyping.
|
|
| 4. |
- Bengtsson, Therese, et al.
(författare)
-
Activation of defence responses to Phytophthora infestans in potato by BABA
- 2014
-
Ingår i: Plant Pathology. - : Wiley. - 0032-0862 .- 1365-3059. ; 63, s. 193-202
-
Tidskriftsartikel (refereegranskat)abstract
- Late blight caused by Phytophthora infestans is one of the most devastating diseases of the potato crop. Resistance breeding and current fungicides are unable to control the rapidly evolving P. infestans and new control strategies are urgently needed. This study examined mechanisms of dl-β-aminobutyric acid (BABA)-induced resistance (IR) in the potato-P. infestans system. Leaves from two cultivars that differ in their degree of resistance, Bintje and Ovatio, were analysed after foliar treatment with BABA. Rapid activation of various defence responses and a significant reduction in P. infestans growth were observed in leaves treated with BABA. In the more resistant cultivar, Ovatio, the activation was both faster and stronger than in Bintje. Microscopic analysis of leaves treated with BABA revealed induction of small hypersensitive response (HR)-like lesions surrounded by callose, as well as production of hydrogen peroxide (H2O2). Molecular and chemical analyses revealed soluble phenols such as arbutin and chlorogenic acid and activation of PR-1. These results show a direct activation of defence responses in potato, rather than priming as reported for other plant species. They also show that the efficiency of BABA-IR differs between cultivars, which highlights the importance of taking all aspects into consideration when establishing new methods for disease management.
|
|
| 5. |
- Bengtsson, Therese, et al.
(författare)
-
Biosurfactants Have the Potential to Induce Defence Against Phytophthora infestans in Potato
- 2015
-
Ingår i: Potato Research. - : Springer Science and Business Media LLC. - 0014-3065 .- 1871-4528. ; 58, s. 83-90
-
Tidskriftsartikel (refereegranskat)abstract
- Potato late blight caused by Phytophthora infestans is one of the most destructive plant diseases worldwide. Currently, its management mainly relies on the frequent use of synthetic chemicals, and there is a need to develop more sustainable strategies. Biosurfactants produced by fluorescent pseudomonads have been shown to rapidly, within 1 min, cause zoospore lyses. This study investigated if the biosurfactant-producing strain Pseudomonas koreensis 2.74 and its biosurfactants have the potential to induce resistance in potato to late blight. The experiments were performed with treatments of whole plants of the susceptible potato cultivar Bintje and the partially resistant cultivar Ovatio. A significant disease reduction and an induced secretion of proteins such as pathogenesis-related (PR) protein 1, within the leaf apoplast, were observed in the potato cultivar Ovatio after treatment with the biosurfactant at a concentration of 1 mg ml(-1). No significant effects on disease development were observed after treatment with the bacterial suspension of Pseudomonas koreensis 2.74.
|
|
| 6. |
|
|
| 7. |
- Bertholdsson, Nils-Ove, et al.
(författare)
-
QTL for chlorophyll fluorescence of barley plants grown at low oxygen concentration in hydroponics to simulate waterlogging
- 2015
-
Ingår i: Euphytica. - : Springer Science and Business Media LLC. - 0014-2336 .- 1573-5060. ; 201, s. 357-365
-
Tidskriftsartikel (refereegranskat)abstract
- Waterlogging is a major factor limiting barley grain yield worldwide. Climate change will likely increase this water stress in Northern Europe. Breeding for waterlogging tolerance (WLT), as for other abiotic stresses, is difficult, but identification of genetic markers linked to genes affecting WLT could facilitate the breeding process. To identify a suitable marker population, parents of 14 double-haploid (DH) barley populations were tested for segregation of biomass growth reduction in waterlogged soil. The most interesting was found in the offspring from crossing cv. Psaknon and breeding line (SLUdt1398 x Mona(4)). Hence, 120 DH-lines derived from this cross were phenotyped for the chlorophyll fluorescence parameter quantum yield (QY) of electron transport of PSII from leaves of hypoxia-stressed plants and further genotyped with 384-SNP Illumina GoldenGate Bead Array. Five quantitative trait loci (QTL) for QY, with a narrow sense heritability of 0.87, were identified on chromosomes 4, 6 and 7H. They had additive effects ranging from 0.74 to 1.35 % with LOD scores from 3 to 12 and explained variance from 6 to 29 %. The major alleles for high QY were from cv. Psaknon; i.e., QY was low if the alleles from cv. Psaknon were not present. Based on leaf necrosis and residual biomass data, the four most interesting QTL may be also in two other populations with completely different progeny, which shows a certain stability of these QTL. The possibility of using marker assistant selection for WLT is discussed, as is possible concurrent improvement of drought tolerance and grain yield.
|
|
| 8. |
- Caspersen, Siri, et al.
(författare)
-
Trädgårdsblåbär och mykorrhiza - sort, substrat och gödsling avgörande
- 2014
-
Ingår i: LTV-fakultetens faktablad.
-
Annan publikation (populärvet., debatt m.m.)abstract
- I Sverige har odlingen av blåbär, båda den kommersiella och i privata trädgårdar, ökat under senare år. Blåbär kräver en sur jord och trivs bra med en hög halt av organiskt material i substratet. I naturen lever blåbär vanligen i symbios med mykorrhizasvampar. Vi har undersökt hur blåbärssorterna Duke, Reka och Northblue påverkas av ympning med mykorrhizapreparat i odlingssubstrat med och utan inblandning av sågspån och med två olika kvävegivor. Effekten av mykorrhizabehandlingen berodde både på blåbärssorten, kvävegivan och odlingssubstratet.
|
|
| 9. |
- Eskandari, Mahboubeh, et al.
(författare)
-
Polyphenol-hydrogen peroxide reactions in skin : In vitro model relevant to study ROS reactions at inflammation
- 2019
-
Ingår i: Analytica Chimica Acta. - : Elsevier. - 0003-2670 .- 1873-4324. ; 1075, s. 91-97
-
Tidskriftsartikel (refereegranskat)abstract
- Antioxidants are important to protect and maintain biological barriers, such as the skin. Antioxidant effects are often assessed using clinical trials, however these tests are costly and time consuming. In this work we introduce a skin membrane-covered oxygen electrode (SCOE) as an in vitro tool for monitoring H2O2 and antioxidant reactions in skin. The SCOE gives amperometric response to H2O2 concentrations down to 0.05 mM. More importantly, the electrode allows measurements of polyphenol penetration and reaction with H2O2 in skin. Measurements with SCOE show that lipophilic polyphenols such as quercetin, piceatannol, resveratrol, and plant extract from Plantago major impose their antioxidant effect in skin within 2-20 min. Rutin is however too hydrophilic to penetrate into stratum corneum and therefore cannot deliver its antioxidant effect during similar time interval. The measurements are interpreted considering polyphenol partition-penetration through stratum corneum and the reaction with the H2O2-catalase system in the skin. The contribution of other enzymes will be addressed in the future. (C) 2019 Elsevier B.V. All rights reserved.
|
|
| 10. |
- Holefors, Anna
(författare)
-
Gene expression changes during short day induced terminal bud formation in Norway spruce
- 2011
-
Ingår i: Plant, Cell and Environment. - : Wiley. - 0140-7791 .- 1365-3040. ; 34, s. 332-346
-
Tidskriftsartikel (refereegranskat)abstract
- The molecular basis for terminal bud formation in autumn is not well understood in conifers. By combining suppression subtractive hybridization and monitoring of gene expression by qRT-PCR analysis, we aimed to identify genes involved in photoperiodic control of growth cessation and bud set in Norway spruce. Close to 1400 ESTs were generated and their functional distribution differed between short day (SD-12 h photoperiod) and long day (LD-24 h photoperiod) libraries. Many genes with putative roles in protection against stress appeared differentially regulated under SD and LD, and also differed in transcript levels between 6 and 20 SDs. Of these, PaTFL1(TERMINAL FLOWER LIKE 1) showed strongly increased transcript levels at 6 SDs. PaCCCH(CCCH-TYPE ZINC FINGER) and PaCBF2&3(C-REPEAT BINDING FACTOR 2&3) showed a later response at 20 SDs, with increased and decreased transcript levels, respectively. For rhythmically expressed genes such as CBFs, such differences might represent a phase shift in peak expression, but might also suggest a putative role in response to SD. Multivariate analyses revealed strong differences in gene expression between LD, 6 SD and 20 SD. The robustness of the gene expression patterns was verified in 6 families differing in bud-set timing under natural light with gradually decreasing photoperiod.
|
|
