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Sökning: WFRF:(Huang Xiao) > Kungliga Tekniska Högskolan

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1.
  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2.
  • Yu, ChaoQing, et al. (författare)
  • Managing nitrogen to restore water quality in China
  • 2019
  • Ingår i: Nature. - : Nature Publishing Group. - 0028-0836 .- 1476-4687. ; 567:7749, s. 516-520
  • Tidskriftsartikel (refereegranskat)abstract
    • The nitrogen cycle has been radically changed by human activities(1). China consumes nearly one third of the world's nitrogen fertilizers. The excessive application of fertilizers(2,3) and increased nitrogen discharge from livestock, domestic and industrial sources have resulted in pervasive water pollution. Quantifying a nitrogen 'boundary'(4) in heterogeneous environments is important for the effective management of local water quality. Here we use a combination of water-quality observations and simulated nitrogen discharge from agricultural and other sources to estimate spatial patterns of nitrogen discharge into water bodies across China from 1955 to 2014. We find that the critical surface-water quality standard (1.0 milligrams of nitrogen per litre) was being exceeded in most provinces by the mid-1980s, and that current rates of anthropogenic nitrogen discharge (14.5 +/- 3.1 megatonnes of nitrogen per year) to fresh water are about 2.7 times the estimated 'safe' nitrogen discharge threshold (5.2 +/- 0.7 megatonnes of nitrogen per year). Current efforts to reduce pollution through wastewater treatment and by improving cropland nitrogen management can partially remedy this situation. Domestic wastewater treatment has helped to reduce net discharge by 0.7 +/- 0.1 megatonnes in 2014, but at high monetary and energy costs. Improved cropland nitrogen management could remove another 2.3 +/- 0.3 megatonnes of nitrogen per year-about 25 per cent of the excess discharge to fresh water. Successfully restoring a clean water environment in China will further require transformational changes to boost the national nutrient recycling rate from its current average of 36 per cent to about 87 per cent, which is a level typical of traditional Chinese agriculture. Although ambitious, such a high level of nitrogen recycling is technologically achievable at an estimated capital cost of approximately 100 billion US dollars and operating costs of 18-29 billion US dollars per year, and could provide co-benefits such as recycled wastewater for crop irrigation and improved environmental quality and ecosystem services.
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3.
  • Tierney, W., et al. (författare)
  • A creative destruction approach to replication : Implicit work and sex morality across cultures
  • 2021
  • Ingår i: Journal of Experimental Social Psychology. - : Elsevier BV. - 0022-1031 .- 1096-0465. ; 93
  • Tidskriftsartikel (refereegranskat)abstract
    • How can we maximize what is learned from a replication study? In the creative destruction approach to replication, the original hypothesis is compared not only to the null hypothesis, but also to predictions derived from multiple alternative theoretical accounts of the phenomenon. To this end, new populations and measures are included in the design in addition to the original ones, to help determine which theory best accounts for the results across multiple key outcomes and contexts. The present pre-registered empirical project compared the Implicit Puritanism account of intuitive work and sex morality to theories positing regional, religious, and social class differences; explicit rather than implicit cultural differences in values; self-expression vs. survival values as a key cultural fault line; the general moralization of work; and false positive effects. Contradicting Implicit Puritanism's core theoretical claim of a distinct American work morality, a number of targeted findings replicated across multiple comparison cultures, whereas several failed to replicate in all samples and were identified as likely false positives. No support emerged for theories predicting regional variability and specific individual-differences moderators (religious affiliation, religiosity, and education level). Overall, the results provide evidence that work is intuitively moralized across cultures.
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4.
  • Zhang, S. N., et al. (författare)
  • The high energy cosmic-radiation detection (HERD) facility onboard China's Space Station
  • 2014
  • Ingår i: Proceedings of SPIE - The International Society for Optical Engineering. - : SPIE. - 9780819496126
  • Konferensbidrag (refereegranskat)abstract
    • The High Energy cosmic-Radiation Detection (HERD) facility is one of several space astronomy payloads of the cosmic lighthouse program onboard China's Space Station, which is planned for operation starting around 2020 for about 10 years. The main scientific objectives of HERD are indirect dark matter search, precise cosmic ray spectrum and composition measurements up to the knee energy, and high energy gamma-ray monitoring and survey. HERD is composed of a 3-D cubic calorimeter (CALO) surrounded by microstrip silicon trackers (STKs) from five sides except the bottom. CALO is made of about 104 cubes of LYSO crystals, corresponding to about 55 radiation lengths and 3 nuclear interaction lengths, respectively. The top STK microstrips of seven X-Y layers are sandwiched with tungsten converters to make precise directional measurements of incoming electrons and gamma-rays. In the baseline design, each of the four side SKTs is made of only three layers microstrips. All STKs will also be used for measuring the charge and incoming directions of cosmic rays, as well as identifying back scattered tracks. With this design, HERD can achieve the following performance: energy resolution of 1% for electrons and gamma-rays beyond 100 GeV, 20% for protons from 100 GeV to 1 PeV; electron/proton separation power better than 10-5; effective geometrical factors of >3 m2sr for electron and diffuse gamma-rays, >2 m2sr for cosmic ray nuclei. R and D is under way for reading out the LYSO signals with optical fiber coupled to image intensified CCD and the prototype of one layer of CALO. 
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5.
  • Fenstermacher, M.E., et al. (författare)
  • DIII-D research advancing the physics basis for optimizing the tokamak approach to fusion energy
  • 2022
  • Ingår i: Nuclear Fusion. - : IOP Publishing. - 0029-5515 .- 1741-4326. ; 62:4
  • Tidskriftsartikel (refereegranskat)abstract
    • DIII-D physics research addresses critical challenges for the operation of ITER and the next generation of fusion energy devices. This is done through a focus on innovations to provide solutions for high performance long pulse operation, coupled with fundamental plasma physics understanding and model validation, to drive scenario development by integrating high performance core and boundary plasmas. Substantial increases in off-axis current drive efficiency from an innovative top launch system for EC power, and in pressure broadening for Alfven eigenmode control from a co-/counter-I p steerable off-axis neutral beam, all improve the prospects for optimization of future long pulse/steady state high performance tokamak operation. Fundamental studies into the modes that drive the evolution of the pedestal pressure profile and electron vs ion heat flux validate predictive models of pedestal recovery after ELMs. Understanding the physics mechanisms of ELM control and density pumpout by 3D magnetic perturbation fields leads to confident predictions for ITER and future devices. Validated modeling of high-Z shattered pellet injection for disruption mitigation, runaway electron dissipation, and techniques for disruption prediction and avoidance including machine learning, give confidence in handling disruptivity for future devices. For the non-nuclear phase of ITER, two actuators are identified to lower the L-H threshold power in hydrogen plasmas. With this physics understanding and suite of capabilities, a high poloidal beta optimized-core scenario with an internal transport barrier that projects nearly to Q = 10 in ITER at ∼8 MA was coupled to a detached divertor, and a near super H-mode optimized-pedestal scenario with co-I p beam injection was coupled to a radiative divertor. The hybrid core scenario was achieved directly, without the need for anomalous current diffusion, using off-axis current drive actuators. Also, a controller to assess proximity to stability limits and regulate β N in the ITER baseline scenario, based on plasma response to probing 3D fields, was demonstrated. Finally, innovative tokamak operation using a negative triangularity shape showed many attractive features for future pilot plant operation.
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6.
  • He, Shiwen, et al. (författare)
  • Energy-Efficient Transceiver Design for Cache-Enabled Millimeter-Wave Systems
  • 2020
  • Ingår i: IEEE Transactions on Communications. - : Institute of Electrical and Electronics Engineers (IEEE). - 0090-6778 .- 1558-0857. ; 68:6, s. 3876-3889
  • Tidskriftsartikel (refereegranskat)abstract
    • In recent years, network densification and edge caching become effective approaches to reduce the burden on the fronthaul links and the content delivery latency for wireless communication systems. However, maximizing system spectral efficiency cannot directly provide any insight on their energy requirements/efficiency for cache-enabled millimeter-wave (mmWave) radio access networks (RANs). In this paper, we study the design of energy-efficient transceiver, consisting of analog and digital precoder/combiner, for the delivery phase of the downlink of cache-enabled mmWave RANs. Due to the non-convexity of the delivery rate and objective, the coupling between the digital and analog precoders/combiners, and the constant module constraint on the elements of analog precoders/combiners, the problem of interest is non-convex and hard to obtain the global optimal solution, even the local optimal solution. To this end, we first overcome these challenges one-by-one and then transform the original problem into tractable one. Finally, an algorithmic framework that converges to the Karush-Kuhn-Tucker solution with provable is developed to achieve the design of energy-efficient transceiver. Numerical results are provided to evaluate the performance of the proposed algorithm, where fully digital precoding is used as benchmark.
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7.
  • Huang, Yiqian, et al. (författare)
  • A Novel Maximum Distance Separable Code Based RIS-OFDM : Design and Optimization
  • 2022
  • Ingår i: 2022 IEEE GLOBAL COMMUNICATIONS CONFERENCE (GLOBECOM 2022). - : Institute of Electrical and Electronics Engineers (IEEE). ; , s. 1521-1526
  • Konferensbidrag (refereegranskat)abstract
    • In this paper, we propose a novel maximum distance separable (MDS) code based and reconfigurable intelligent surface (RIS) assisted wireless communication system with orthogonal frequency division multiplexing (OFDM). Specifically, input bits are firstly divided into groups and their MDS codes are utilized to decide the amplitudes and phases of subcarriers. The introduction of the MDS code helps to increase the minimum Hamming distance between symbols and improve on the capability of error detection. Besides, the RIS is adopted to create additional paths between the radio frequency (RF) and the receiver as well as alter the signal phases with derived optimal solution. Benefiting from the strength of the RIS, the proposed system can better overcome multipath fading compared with conventional systems. Simulation results are presented to demonstrate the efficacy of the proposed system in terms of reducing bit error rate (BER) through multipath channels.
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8.
  • Xiao, Hanshen, et al. (författare)
  • Robustness in Chinese Remainder Theorem for Multiple Numbers and Remainder Coding
  • 2018
  • Ingår i: IEEE Transactions on Signal Processing. - : Institute of Electrical and Electronics Engineers (IEEE). - 1053-587X .- 1941-0476. ; 66:16, s. 4347-4361
  • Tidskriftsartikel (refereegranskat)abstract
    • Chinese remainder theorem (CRT) has been widely studied with its applications in frequency estimation, phase unwrapping, coding theory, and distributed data storage. Since traditional CRT is greatly sensitive to the errors in residues due to noises, the problem of robustly reconstructing integers via the erroneous residues has been intensively studied in the literature. In order to robustly reconstruct integers, there are basically two approaches: one is to introduce common divisors in the moduli and the other is to directly decrease the dynamic range. In this paper, we take further insight into the geometry property of the linear space associated with CRT. Echoing both ways to introduce redundancy, we propose a pseudometric as a uniform framework to analyze the tradeoff between the error bound and the dynamic range for robust CRT. Furthermore, we present the first robust CRT for multiple numbers to solve the problem raised by CRT-based undersampling frequency estimation in general. Based on symmetric polynomials proposed, we proved that in most cases, the problem can he solved efficiently in the polynomial time.
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9.
  • Xiao, Yue, et al. (författare)
  • Fully Decentralized Federated Learning-Based On-Board Mission for UAV Swarm System
  • 2021
  • Ingår i: IEEE Communications Letters. - : Institute of Electrical and Electronics Engineers (IEEE). - 1089-7798 .- 1558-2558. ; 25:10, s. 3296-3300
  • Tidskriftsartikel (refereegranskat)abstract
    • To handle the data explosion in the era of Internet-of-things, it is of interest to investigate the decentralized network, with the aim at relaxing the burden at the central server along with preserving data privacy. In this work, we develop a fully decentralized federated learning (FL) framework with an inexact stochastic parallel random walk alternating direction method of multipliers (ISPW-ADMM). Performing more efficient communication and enhanced privacy preservation compared with the current state-of-the-art, the proposed ISPW-ADMM can be partially immune to the effect of time-varying dynamic network and stochastic data collection, while still in fast convergence. Benefiting from the stochastic gradients and biased first-order moment estimation, the proposed framework can be applied to any decentralized FL tasks over time-varying graphs. Thus, to demonstrate the practicability of such a framework in providing fast convergence, high communication efficiency, noise robustness for a specific on-board mission to some extent, we study the extreme learning machine-based FL model beamforming design in unmanned aerial vehicle communications, as verified by the numerical simulations.
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10.
  • Zhang, Jian, et al. (författare)
  • Optimization of N-Substituted Oseltamivir Derivatives as Potent Inhibitors of Group-1 and-2 Influenza A Neuraminidases, Including a Drug-Resistant Variant
  • 2018
  • Ingår i: Journal of Medicinal Chemistry. - : American Chemical Society (ACS). - 0022-2623 .- 1520-4804. ; 61:14, s. 6379-6397
  • Tidskriftsartikel (refereegranskat)abstract
    • On the basis of our earlier discovery of N1-selective inhibitors, the 150-cavity of influenza virus neuraminidases (NAs) could be further exploited to yield more potent oseltamivir derivatives. Among the synthesized compounds, 15b and 15c were exceptionally active against both group-1 and -2 NAs. Especially for 09N1, N2, N6, and N9 subtypes, they showed 6.80-12.47 and 1.20-3.94 times greater activity than oseltamivir carboxylate (OSC). They also showed greater inhibitory activity than OSC toward H274Y and E119V variant. In cellular assays, they exhibited greater potency than OSC toward H5N1, H5N2, H5N6, and H5N8 viruses. 15b demonstrated high metabolic stability, low cytotoxicity in vitro, and low acute toxicity in mice. Computational modeling and molecular dynamics studies provided insights into the role of R group of 15b in improving potency toward group-1 and -2 NAs. We believe the successful exploitation of the 150-cavity of NAs represents an important breakthrough in the development of more potent anti-influenza agents.
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