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Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

Adewumi, Oluseun (author)
Aflatoonian, Behrouz (author)
Ahrlund-Richter, Lars (author)
Karolinska Institutet
show more...
Amit, Michal (author)
Andrews, Peter W. (author)
Beighton, Gemma (author)
Bello, Paul A. (author)
Benvenisty, Nissim (author)
Berry, Lorraine S. (author)
Bevan, Simon (author)
Blum, Barak (author)
Brooking, Justin (author)
Chen, Kevin G. (author)
Choo, Andre B. H. (author)
Churchill, Gary A. (author)
Corbel, Marie (author)
Damjanov, Ivan (author)
Draper, Jon S. (author)
Dvorak, Petr (author)
Emanuelsson, Katarina (author)
Fleck, Roland A. (author)
Ford, Angela (author)
Gertow, Karin (author)
Gertsenstein, Marina (author)
Gokhale, Paul J. (author)
Hamilton, Rebecca S. (author)
Hampl, Ales (author)
Healy, Lyn E. (author)
Hovatta, Outi (author)
Karolinska Institutet
Hyllner, Johan (author)
Imreh, Marta P. (author)
Karolinska Institutet,Uppsala universitet,Zoologisk utvecklingsbiologi
Itskovitz-Eldor, Joseph (author)
Jackson, Jamie (author)
Johnson, Jacqueline L. (author)
Jones, Mark (author)
Kee, Kehkooi (author)
King, Benjamin L. (author)
Knowles, Barbara B. (author)
Lako, Majlinda (author)
Lebrin, Franck (author)
Mallon, Barbara S. (author)
Manning, Daisy (author)
Mayshar, Yoav (author)
Mckay, Ronald D. G. (author)
Michalska, Anna E. (author)
Mikkola, Milla (author)
Mileikovsky, Masha (author)
Minger, Stephen L. (author)
Moore, Harry D. (author)
Mummery, Christine L. (author)
Nagy, Andras (author)
Nakatsuji, Norio (author)
O'Brien, Carmel M. (author)
Oh, Steve K. W. (author)
Olsson, Cia (author)
Otonkoski, Timo (author)
Park, Kye-Yoon (author)
Passier, Robert (author)
Patel, Hema (author)
Patel, Minal (author)
Pedersen, Roger (author)
Pera, Martin F. (author)
Piekarczyk, Marian S. (author)
Pera, Renee A. Reijo (author)
Reubinoff, Benjamin E. (author)
Robins, Allan J. (author)
Rossant, Janet (author)
Rugg-Gunn, Peter (author)
Schulz, Thomas C. (author)
Semb, Henrik (author)
Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine
Sherrer, Eric S. (author)
Siemen, Henrike (author)
Stacey, Glyn N. (author)
Stojkovic, Miodrag (author)
Suemori, Hirofumi (author)
Szatkiewicz, Jin (author)
Turetsky, Tikva (author)
Tuuri, Timo (author)
van den Brink, Steineke (author)
Vintersten, Kristina (author)
Vuoristo, Sanna (author)
Ward, Dorien (author)
Weaver, Thomas A. (author)
Young, Lesley A. (author)
Zhang, Weidong (author)
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 (creator_code:org_t)
2007-06-17
2007
English.
In: Nature Biotechnology. - : Springer Science and Business Media LLC. - 1087-0156 .- 1546-1696. ; 25:7, s. 803-816
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue- nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Keyword

MEDICINE
MEDICIN

Publication and Content Type

ref (subject category)
art (subject category)

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By the author/editor
Adewumi, Oluseun
Aflatoonian, Beh ...
Ahrlund-Richter, ...
Amit, Michal
Andrews, Peter W ...
Beighton, Gemma
show more...
Bello, Paul A.
Benvenisty, Niss ...
Berry, Lorraine ...
Bevan, Simon
Blum, Barak
Brooking, Justin
Chen, Kevin G.
Choo, Andre B. H ...
Churchill, Gary ...
Corbel, Marie
Damjanov, Ivan
Draper, Jon S.
Dvorak, Petr
Emanuelsson, Kat ...
Fleck, Roland A.
Ford, Angela
Gertow, Karin
Gertsenstein, Ma ...
Gokhale, Paul J.
Hamilton, Rebecc ...
Hampl, Ales
Healy, Lyn E.
Hovatta, Outi
Hyllner, Johan
Imreh, Marta P.
Itskovitz-Eldor, ...
Jackson, Jamie
Johnson, Jacquel ...
Jones, Mark
Kee, Kehkooi
King, Benjamin L ...
Knowles, Barbara ...
Lako, Majlinda
Lebrin, Franck
Mallon, Barbara ...
Manning, Daisy
Mayshar, Yoav
Mckay, Ronald D. ...
Michalska, Anna ...
Mikkola, Milla
Mileikovsky, Mas ...
Minger, Stephen ...
Moore, Harry D.
Mummery, Christi ...
Nagy, Andras
Nakatsuji, Norio
O'Brien, Carmel ...
Oh, Steve K. W.
Olsson, Cia
Otonkoski, Timo
Park, Kye-Yoon
Passier, Robert
Patel, Hema
Patel, Minal
Pedersen, Roger
Pera, Martin F.
Piekarczyk, Mari ...
Pera, Renee A. R ...
Reubinoff, Benja ...
Robins, Allan J.
Rossant, Janet
Rugg-Gunn, Peter
Schulz, Thomas C ...
Semb, Henrik
Sherrer, Eric S.
Siemen, Henrike
Stacey, Glyn N.
Stojkovic, Miodr ...
Suemori, Hirofum ...
Szatkiewicz, Jin
Turetsky, Tikva
Tuuri, Timo
van den Brink, S ...
Vintersten, Kris ...
Vuoristo, Sanna
Ward, Dorien
Weaver, Thomas A ...
Young, Lesley A.
Zhang, Weidong
show less...
About the subject
MEDICAL AND HEALTH SCIENCES
MEDICAL AND HEAL ...
and Basic Medicine
and Cell and Molecul ...
Articles in the publication
Nature Biotechno ...
By the university
Uppsala University
Karolinska Institutet
Lund University

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