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- Jacobsson, Benny, 1954-
(author)
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Den sjunde världsdelen : Västgötar och Västergötland 1646-1771. En identitetshistoria
- 2008
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Doctoral thesis (other academic/artistic)abstract
- This thesis considers regional identity in early-modern Sweden, taking its case from the province (landskap) of Västergötland 1646–1771. The aim is to investigate verbal expressions of regional identity. A theory on the construction of regional identity is suggested from the research results, and typological categories of regional identity are established. Contrary to popular concepts of identity being constructed in relation to an outer “other”, it is argued that identity is formed from self-images. Identity is expressed in the first person (I, we), and includes an insider’s perspective of the place occupied. As the thesis shows, the theory of regional identity is substantiated by the duality of the patria-concept. Patria, Fatherland, was employed for the smaller home province as well as for the greater realm. The realm however, always could claim priority to amor patria, Love of the Fatherland. Thus, any construction of regional identity using the neighbouring provinces as a contrasting “other” would have been counter-productive to the construction of the overarching national identity. This manifold patria-concept is of Roman origin, and made its influence through the Latin language well into early-modern time. A great variety of sources in Latin and Swedish have been consulted, including orations and dissertations, minutes of the academic senate and West Geat student nation (Västgöta nation) at Uppsala University, topography, manuscripts and letters. An in-depth study has been made of the writing processes leading up to dissertations and topographical descriptions.
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- Kölhed, Malin, 1977-
(author)
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Novel on-line mid infrared detection strategies in capillary electrophoretic systems
- 2005
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Doctoral thesis (other academic/artistic)abstract
- Infrared absorption spectra can provide analytically useful information on a large variety of compounds, ranging from small ions to large biological molecules. In fact, all analytes that possess a dipole moment that changes during vibration are infrared-active. The infrared (IR) spectrum can be subdivided into far-, mid- and near- regions. The focus of attention in this thesis is the mid-IR region, in which the fundamental vibrations of most organic compounds are located, thus providing scope for positive structural identification. However, while such near-ubiquitous signals can be very useful for monitoring simple molecules in simple systems, they can be increasingly disadvantageous as the number of analytes and/or the complexity of the sample matrix increases. Thus, hyphenation to a separation system prior to detection is desirable. Paper I appended to this thesis presents (for the first time) the on-line hyphenation between Fourier transform infrared spectroscopy, FTIR, and capillary zone electrophoresis, CZE. CZE is a highly efficient separation technique that separates ionic analytes with respect to their charge-to-size ratio. It is most commonly performed in aqueous buffers in fused silica capillaries. Since these capillaries absorb virtually all infrared light an IR-transparent flow cell had to be developed. In further studies (Paper II) the applicability of CZE is expanded to include neutral analytes by the addition of micelles to the buffer, and micellar electrokinetic chromatography, MEKC, was successfully hyphenated to FTIR for the first time. Paper III describes an application of the on-line CZE-FTIR technique in which non-UV-absorbing analytes in a complex matrix were separated, identified and quantified in one run.Measuring aqueous solutions in the mid-IR region is not straightforward since water absorbs intensely in this region, sometimes completely, leaving no transmitted, detectable light. For this reason, quantum cascade lasers are interesting. These lasers represent a new type of mid-IR semiconducting lasers with high output power due to their ingenious design. The laser action lies within one conduction band (intersubband) and can be tailored to emit light in the entire mid-IR region using the same semiconducting material. To investigate their potential to increase the optical path length in aqueous solutions, these lasers were used with an aqueous flow system (Paper IV), and the experience gained in these experiments enabled hyphenation of such lasers to a CZE system (Paper V).
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- Röbeck, Pontus, et al.
(author)
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Multiplex protein analysis and ensemble machine learning methods of fine needle aspirates from prostate cancer patients reveal potential diagnostic signatures associated with tumour grade
- 2023
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In: Cytopathology. - : Wiley. - 0956-5507 .- 1365-2303. ; 34:4, s. 286-294
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Journal article (peer-reviewed)abstract
- Background: Improved molecular diagnosis is needed in prostate cancer (PC). Fine needle aspiration (FNA) is a minimally invasive biopsy technique, less traumatic compared to core needle biopsy, and could be useful for diagnosis of PC. Molecular biomarkers (BMs) in FNA-samples can be assessed for prediction, eg of immunotherapy efficacy before treatment as well as at treatment decision time points during disease progression.Methods: In the present pilot study, the expression levels of 151 BM proteins were analysed by proximity extension assay in FNA-samples from 16 patients, including benign prostate lesions (n = 3) and cancers (n = 13). An ensemble data analysis strategy was applied using several machine learning models.Results: Twelve potentially predictive BM proteins correlating with International Society of Urological Pathology grade groups were identified, among them vimentin, tissue factor pathway inhibitor 2, and integrin beta-5. The validity of the results was supported by network analysis that showed functional associations between most of the identified putative BMs. We also showed that multiple immune checkpoint targets can be assessed (eg PD-L1, CD137, and Galectin-9), which may support the selection of immunotherapy in advanced PC. Results are promising but need further validation in a larger cohort.Conclusions: Our pilot study represents a “proof of concept” and shows that multiplex profiling of potential diagnostic and predictive BM proteins is feasible on tumour material obtained by FNA sampling of prostate cancer. Moreover, our results demonstrate that an ensemble data analysis strategy may facilitate the identification of BM signatures in pilot studies when the patient cohort is limited.
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