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Correlative STED su...
Correlative STED super-resolution light and electron microscopy on resin sections
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- Wurm, Christian A. (författare)
- Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany.;Abberior Instruments GmbH, Gottingen, Germany.
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- Schwarz, Heinz (författare)
- Max Planck Inst Dev Biol, Tubingen, Germany.
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- Jans, Daniel C. (författare)
- KTH,Tillämpad fysik,Science for Life Laboratory, SciLifeLab,Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany.;Univ Med Ctr Gottingen, Clin Neurol, Gottingen, Germany
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- Riedel, Dietmar (författare)
- Max Planck Inst Biophys Chem, Lab Electron Microscopy, D-37077 Gottingen, Germany.
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- Humbel, Bruno M. (författare)
- Univ Lausanne, Electron Microscopy Facil, Lausanne, Switzerland.;Okinawa Inst Sci & Technol, Imaging, Onna Son, Okinawa, Japan.
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- Jakobs, Stefan (författare)
- Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany.;Univ Med Ctr Gottingen, Clin Neurol, Gottingen, Germany.
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Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany;Abberior Instruments GmbH, Gottingen, Germany. Max Planck Inst Dev Biol, Tubingen, Germany. (creator_code:org_t)
- 2019-07-16
- 2019
- Engelska.
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Ingår i: Journal of Physics D. - : Institute of Physics (IOP). - 0022-3727 .- 1361-6463. ; 52:37
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Correlative light and electron microscopy approaches can reveal the localisation of specific proteins while providing detailed information on the cellular context, thereby combining the strengths of both imaging modalities. The major challenge in combining fluorescence microscopy with electron microscopy is the different sample preparation requirements necessary for obtaining high quality data from both modalities. To overcome this limitation, we combined conventional sample preparation protocols for electron microscopy with post-embedding labelling on ultra-thin sections using antibodies and other specific ligands. We successfully employed STED super-resolution microscopy to image the subcellular distribution of several targets in various specimen including E. coli, T brucei, S. cerevisiae, human cancer cells and bovine sperm. Thus, we present widely applicable methods facilitating the use of antibodies for correlative super-resolution light and electron microscopy of post-embedding labelled targets.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
Nyckelord
- CLEM
- STED
- super-resolution microscopy
- immunolabelling
- immunogold
- resin embedding
- correlative microscopy
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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