| 1. |
- Xia, Yutao, 1995, et al.
(författare)
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Aerodynamic influences of typical windbreak wall types on a high-speed train under crosswinds
- 2022
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Ingår i: Journal of Wind Engineering and Industrial Aerodynamics. - : Elsevier BV. - 0167-6105. ; 231
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Tidskriftsartikel (refereegranskat)abstract
- The effect of windbreak wall types on their windproof effects on trains has been systematically investigated by comparing the flow field around a high-speed train behind three typical windbreak walls. These three typical windbreak walls are widely used along the windy railway lines in China, which include the earth embankment–type windbreak wall (EW), the road cutting–type windbreak wall (RW), and the straight reinforced concrete–type windbreak wall (SW). We compared the time-averaged crosswind-induced flows and aerodynamic performances of high-speed trains behind these three windbreak walls with the same height using numerical simulations. The results revealed that the windbreak wall geometric shape effects on the aerodynamic load coefficients varied according to the type of aerodynamic load and carriage marshalling positions but barely varied with yaw angles. The total drag coefficients of the train in the EW and RW were approximately 50–60% of that in the SW under the two smaller yaw angles and were only 30–40% for the largest yaw angle. For the absolute value of the side force and rolling moments coefficients, the maximum values for the head and middle cars both appeared in the RW, and the corresponding minimum values were obtained in the SW. The maximum and minimum values for the tail car were obtained at the SW and EW, respectively. The maximum of the rolling moment coefficient among three carriages in the SW was approximately 45–60% of that in the EW and only 30–40% of that in the RW. The time-averaged train surface pressure coefficient and flow patterns were similar between the EW and RW, which showed apparent differences from those in the SW. The SW provided a strong blocking effect on the incoming windward airflow and avoided the direct impact on the train. Compared with the RW, the uplifting effect of the EW's windward slope on the incoming flow further reduced the crosswind effect on trains. In addition, the dominating frequency characteristics of the aerodynamic loads were significantly affected by windbreak wall types. These findings provide a systematic understanding of the time-averaged aerodynamics of trains behind these three typical windbreak walls.
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| 2. |
- Ma, Tian, et al.
(författare)
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Lipid engineering combined with systematic metabolic engineering of Saccharomyces cerevisiae for high-yield production of lycopene
- 2019
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Ingår i: Metabolic Engineering. - : Elsevier BV. - 1096-7176 .- 1096-7184. ; 52, s. 134-142
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Tidskriftsartikel (refereegranskat)abstract
- Saccharomyces cerevisiae is an efficient host for natural-compound production and preferentially employed in academic studies and bioindustries. However, S. cerevisiae exhibits limited production capacity for lipophilic natural products, especially compounds that accumulate intracellularly, such as polyketides and carotenoids, with some engineered compounds displaying cytotoxicity. In this study, we used a nature-inspired strategy to establish an effective platform to improve lipid oil–triacylglycerol (TAG) metabolism and enable increased lycopene accumulation. Through systematic traditional engineering methods, we achieved relatively high-level production at 56.2 mg lycopene/g cell dry weight (cdw). To focus on TAG metabolism in order to increase lycopene accumulation, we overexpressed key genes associated with fatty acid synthesis and TAG production, followed by modulation of TAG fatty acyl composition by overexpressing a fatty acid desaturase (OLE1) and deletion of Seipin (FLD1), which regulates lipid-droplet size. Results showed that the engineered strain produced 70.5 mg lycopene/g cdw, a 25% increase relative to the original high-yield strain, with lycopene production reaching 2.37 g/L and 73.3 mg/g cdw in fed-batch fermentation and representing the highest lycopene yield in S. cerevisiae reported to date. These findings offer an effective strategy for extended systematic metabolic engineering through lipid engineering.
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| 3. |
- Qin, Ning, et al.
(författare)
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Increased CO 2 fixation enables high carbon-yield production of 3-hydroxypropionic acid in yeast
- 2024
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Ingår i: Nature Communications. - 2041-1723 .- 2041-1723. ; 15:1
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Tidskriftsartikel (refereegranskat)abstract
- CO2 fixation plays a key role to make biobased production cost competitive. Here, we use 3-hydroxypropionic acid (3-HP) to showcase how CO2 fixation enables approaching theoretical-yield production. Using genome-scale metabolic models to calculate the production envelope, we demonstrate that the provision of bicarbonate, formed from CO2, restricts previous attempts for high yield production of 3-HP. We thus develop multiple strategies for bicarbonate uptake, including the identification of Sul1 as a potential bicarbonate transporter, domain swapping of malonyl-CoA reductase, identification of Esbp6 as a potential 3-HP exporter, and deletion of Uga1 to prevent 3-HP degradation. The combined rational engineering increases 3-HP production from 0.14 g/L to 11.25 g/L in shake flask using 20 g/L glucose, approaching the maximum theoretical yield with concurrent biomass formation. The engineered yeast forms the basis for commercialization of bio-acrylic acid, while our CO2 fixation strategies pave the way for CO2 being used as the sole carbon source.
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| 4. |
- Fu, Le, et al.
(författare)
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Transparent single crystalline ZrO2-SiO2 glass nanoceramic sintered by SPS
- 2016
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Ingår i: Journal of the European Ceramic Society. - : Elsevier BV. - 0955-2219 .- 1873-619X. ; 36:14, s. 3487-3494
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Tidskriftsartikel (refereegranskat)abstract
- Transparent ZrO2-SiO2 glass ceramics show potential for application in the dental industry. The application of this material prepared by a sol-gel method was hindered by the difficulties in obtaining large dimension samples. Spark plasma sintering (SPS) offers the possibility of achieving transparent ZrO2-SiO2 glass ceramics. In this study, a ZrO2-SiO2 powder was prepared by the sol-gel method and subsequently sintered by SPS. Varied sintering temperatures and pressures were explored to achieve better mechanical strength and transparency. TEM results showed single crystalline ZrO2 spherical nanocrystals (approximately 20 nm) homogenously embedded in the SiO2 matrix. Tetragonal ZrO2 was the only crystalline phase in all specimens. With sintering conditions of 1200 degrees C and 30 MPa, a glass ceramic with fracture toughness of 4.13 MPa was obtained. This value is similar to the commercial dental glass ceramic of IPS e.max (R) Press. The studied transparent glass ceramic with high transparency and moderate mechanical strength shows promise for dental application.
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| 5. |
- Shi, Lei, et al.
(författare)
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An investigation into ultra-thin pseudobinary oxide (TiO2)(x)(Al2O3)(1-x) films as high-k gate dielectrics
- 2008
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Ingår i: Applied Physics A. - : Springer Verlag (Germany). - 0947-8396 .- 1432-0630. ; 90:2, s. 379-384
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Tidskriftsartikel (refereegranskat)abstract
- As potential gate dielectric materials, pseudobinary oxide (TiO2)(x)(Al2O3)(1-x) (0.1 less than= x less than= 0.6) films (TAO) were deposited on Si (100) substrates by pulsed-laser deposition method and studied systematically via various measurements. By a special deposition process, including two separate steps, the TAO films were deposited in the form of two layers. The first layer was deposited at room temperature and the second layer was completed at the substrate temperature of 400 degrees C. Detailed data show that the properties of the TAO films are closely related to the ratio between TiO2 and Al2O3. The existence of the first layer deposited at room temperature can effectively restrain the formation of the interfacial layer. And according to the results of X-ray photoelectron spectroscopy and high-resolution transmission electron microscopy performed on the films, no other information belonging to the silicon oxide could be observed. For the (TiO2)(0.4)(Al2O3)(0.6) film, the best result has been achieved among all samples and its dielectric constant is evaluated to be about 38. It is valuable for the amorphous TAO film as one of the promising dielectric materials for high-k gate dielectric applications.
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| 6. |
- Xia, Q. L., et al.
(författare)
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Infrared Laser Pulses Excite Action Potentials in Primary Cortex Neurons in Vitro∗
- 2019
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Ingår i: Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. ; , s. 5184-5187
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Konferensbidrag (refereegranskat)abstract
- Infrared neural modulation (INM) has been well studied in the peripheral nervous system (PNS) for potential clinical applications. However, limited research has been conducted on the central nervous systems (CNS). In this study, we aimed at investigating the feasibility of using pulsed infrared (IR) laser with a wavelength of 1940 nm to excite network activity of cultivated rat cortex neurons.We cultured rat cortex neurons, forming neural networks with spontaneous neural activity, on glass multi-electrode arrays (MEAs). Laser at a power of 600 mW and a pulse rate of 10 Hz were used to stimulate the neural networks using the optics of an inverted microscope. Pulse durations were varied from 200 μs to 1 ms. The spike rate was calculated to evaluate the change of the neural network activity during the IR stimuli and the corresponding frequency components of neural response were calculated to examine whether recorded spikes were evoked by the IR pulse or not. A temperature model was adapted from a previous study to estimate the temperature rise during laser pulsing.We observed that the IR irradiation with a pulse duration of 800 μs and 1 ms could excite neuronal action potentials. The temperature rose 18.5 and 23.9 °C, for pulse durations of 800 μs and 1 ms, respectively. Thus, in addition to previously shown inhibition of IR irradiation with a wavelength of 1550 nm, we demonstrate an optical method that can modulate neural network activity in vitro. The preliminary results from this paper also suggested that MEA recording technology coupled with a laser and microscope systems can be exploited as a new approach for future studies to understand mechanisms and characterize laser parameters of INM for CNS neurons.
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| 7. |
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| 8. |
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| 9. |
- Cheng, Yirui, et al.
(författare)
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Comparison of serum exosome isolation methods on co-precipitated free microRNAs.
- 2020
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Ingår i: PeerJ. - : PeerJ. - 2167-8359. ; 8
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Tidskriftsartikel (refereegranskat)abstract
- Background: Exosomes are nano-sized extracellular vesicles containing different biomolecules such as proteins and microRNAs (miRNAs) that mediate intercellular communication. Recently, numerous studies have reported the important functions of exosomal miRNAs in disease development and the potential clinical application as diagnostic biomarkers. Up to now, the most commonly used methods to extract exosomes are ultracentrifugation (UC) and precipitation-based commercial kit (e.g., ExoQuick). Generally, both UC and ExoQuick method could co-isolate contaminating proteins along with exosomes, with the UC method yielding even purer exosomes than ExoQuick. However, the comparison of these two methods on co-precipitated free miRNAs is still unknown.Methods: In this study, we isolated exosomes from the human serum with exogenously added cel-miR-39 by UC and ExoQuick and compared the proportion of cel-miR-39 co-precipitated with exosomes extracted by these two methods.Results: Using exogenous cel-miR-39 as free miRNAs in serum, we concluded that ExoQuick co-isolates a small proportion of free miRNAs while UC hardly precipitates any free miRNAs. We also found that incubation at 37 °C for 1 h could decrease the proportion of free miRNAs, and exosomal miRNAs like miR-126 and miR-152 also decreased when RNase A was used. In conclusion, our findings provide essential information about the details of serum exosome isolation methods for further research on exosomal miRNAs.
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| 10. |
- Fu, Le, et al.
(författare)
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Doping of tantalum, niobium, and hafnium in a translucent ZrO2-SiO2 nanocrystalline glass-ceramic
- 2022
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Ingår i: Journal of the European Ceramic Society. - : Elsevier. - 0955-2219 .- 1873-619X. ; 42:4, s. 1731-1742
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Tidskriftsartikel (refereegranskat)abstract
- The addition of dopant(s) is an effective strategy to regulate the microstructure and properties of ZrO2-based ceramics. In this study, we investigated the effects of ternary element alloying, namely tantalum (Ta), niobium (Nb), and hafnium (Hf) elements, on the microstructure and transformability of ZrO2 nanocrystallites in a ZrO2SiO2 nanocrystalline glass-ceramic (NCGC) during sintering and thermal treatments. The ternary dopants enhanced the transformability of tetragonal ZrO2 (t-ZrO2) nanocrystallites during sintering, i.e., the dopants acted as t-ZrO2 destabilizer. The Ta, Nb and Hf elements dissolved in ZrO2 nanocrystallites, forming ZrO2 solid solution. Meanwhile, lamella nanotwins were formed within many ZrO2 nanocrystallites. No obvious segregation of dopants was detected at ZrO2 grain boundaries. t-ZrO2 and monoclinic (m) ZrO2 nanocrystallites were metastable in thermal treatments process, with "t" to "m" and the reverse "m" to "t" polymorphic transformation occurred simultaneously. Meanwhile, t-ZrO2 and m-ZrO2 nanocrystallites had a great tendency to grow larger during thermal treatments.
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