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Sökning: WFRF:(Jimenez Esteban F.) > Chalmers tekniska högskola

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1.
  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2.
  • Engels, D., et al. (författare)
  • Variability of masers in circumstellar shells on timescales of decades
  • 2007
  • Ingår i: Proceedings of the International Astronomical Union. - 1743-9213 .- 1743-9221. ; 3:S242, s. 318-319
  • Konferensbidrag (refereegranskat)abstract
    • Based on a 15-20 year long monitoring program of H2O masers and new observations of OH masers discovered 35 years ago, we studied the variability of maser emission in AGB stars over timescales of decades. The H2O maser features in the semi-regular variable stars RX Boo and SV Peg, and of the Mira stars U Her and R Cas, showed strong fluctuations superposed in case of the Mira stars on regular variations due to the pulsation of the stars. The spatial distribution of the emission regions in RX Boo and U Her showed deviations from spherical symmetry, which remained unchanged over >10 years. We conclude that the spatial asymmetry is determined by the underlying asymmetry of the mass loss process. There is no evidence that mass loss rates or the wind geometry in these stars have varied over the last 20 years. In 2005 we re-detected at 1612 MHz the 114 OH/IR stars discovered before 1978, implying an OH maser lifetime of >2800 years (1). © 2008 International Astronomical Union.
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