| 1. |
- Ståhl, Patrik L., et al.
(författare)
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Translational Database Selection and Multiplexed Sequence Capture for Up Front Filtering of Reliable Breast Cancer Biomarker Candidates
- 2011
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 6:6, s. e20794-
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Tidskriftsartikel (refereegranskat)abstract
- Biomarker identification is of utmost importance for the development of novel diagnostics and therapeutics. Here we make use of a translational database selection strategy, utilizing data from the Human Protein Atlas (HPA) on differentially expressed protein patterns in healthy and breast cancer tissues as a means to filter out potential biomarkers for underlying genetic causatives of the disease. DNA was isolated from ten breast cancer biopsies, and the protein coding and flanking non-coding genomic regions corresponding to the selected proteins were extracted in a multiplexed format from the samples using a single DNA sequence capture array. Deep sequencing revealed an even enrichment of the multiplexed samples and a great variation of genetic alterations in the tumors of the sampled individuals. Benefiting from the upstream filtering method, the final set of biomarker candidates could be completely verified through bidirectional Sanger sequencing, revealing a 40 percent false positive rate despite high read coverage. Of the variants encountered in translated regions, nine novel non-synonymous variations were identified and verified, two of which were present in more than one of the ten tumor samples.
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| 2. |
- Hall, Ola, et al.
(författare)
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Classification of Maize in Complex Smallholder Farming Systems Using UAV Imagery
- 2018
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Ingår i: Drones. - : MDPI AG. - 2504-446X. ; 2:3, s. 1-8
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Tidskriftsartikel (refereegranskat)abstract
- Yield estimates and yield gap analysis are important for identifying poor agricultural productivity. Remote sensing holds great promise for measuring yield and thus determining yield gaps. Farming systems in sub-Saharan Africa (SSA) are commonly characterized by small field size, intercropping, different crop species with similar phenologies, and sometimes high cloud frequency during the growing season, all of which pose real challenges to remote sensing. Here, an unmanned aerial vehicle (UAV) system based on a quadcopter equipped with two consumer-grade cameras was used for the delineation and classification of maize plants on smallholder farms in Ghana. Object-oriented image classification methods were applied to the imagery, combined with measures of image texture and intensity, hue, and saturation (IHS), in order to achieve delineation. It was found that the inclusion of a near-infrared (NIR) channel and red–green–blue (RGB) spectra, in combination with texture or IHS, increased the classification accuracy for both single and mosaic images to above 94%. Thus, the system proved suitable for delineating and classifying maize using RGB and NIR imagery and calculating the vegetation fraction, an important parameter in producing yield estimates for heterogeneous smallholder farming systems.
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| 3. |
- Nyberg, Gert, et al.
(författare)
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Enclosures in West Pokot, Kenya: Transforming land, livestock and livelihoods in drylands
- 2015
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Ingår i: Pastoralism. - : Springer Science and Business Media LLC. - 2041-7136 .- 2041-7128. ; 5:1
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Tidskriftsartikel (refereegranskat)abstract
- Dryland livestock production systems are changing in many parts of the world, as a result of growing human populations and associated pressure on water and land. Based on a combination of social and natural science methods, we studied a 30-year transformation process from pastoralism to a livestock-based agro-pastoral system in northwestern Kenya, with the overall aim to increase the understanding of the ongoing transition towards intensified agro-pastoralist production systems in dryland East Africa.Key to this transformation was the use of enclosures for land rehabilitation, fodder production, and land and livestock management. Enclosures have more soil carbon and a higher vegetation cover than adjacent areas with open grazing. The level of adoption of enclosures as a management tool has been very high, and their use has enabled agricultural diversification, e.g. increased crop agriculture, poultry production and the inclusion of improved livestock. Following the use of enclosures, livelihoods have become less dependent on livestock migration, are increasingly directed towards agribusinesses and present new opportunities and constraints for women. These livelihood changes are closely associated with, and depend on, an ongoing privatization of land under different tenure regimes.The results indicate that the observed transformation provides opportunities for a pathway towards a sustainable livestock-based agro-pastoral system that could be valid in many dryland areas in East Africa. However, we also show that emergent risks of conflicts and inequalities in relation to land, triggered by the weakening of collective property rights, pose a threat to the sustainability of this pathway.
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