| 1. |
- Cano, María Emilia, et al.
(författare)
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Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum
- 2024
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Ingår i: International Journal of Biological Macromolecules. - : Elsevier B.V.. - 0141-8130 .- 1879-0003. ; 267
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Tidskriftsartikel (refereegranskat)abstract
- Biomass-derived oligo- and polysaccharides may act as elicitors, i.e., bioactive molecules that trigger plant immune responses. This is particularly important to increase the resistance of plants to abiotic and biotic stresses. In this study, cellulose nanofibrils (CNF) gels were obtained by TEMPO-mediated oxidation of unbleached and bleached kraft pulps. The molecular structures were characterized with ESI and MALDI MS. Analysis of the fine sequences was achieved by MS and MS/MS of the water-soluble oligosaccharides obtained by acid hydrolysis of the CNF gels. The analysis revealed the presence of two families: one corresponding to homoglucuronic acid sequences and the other composed by alternating glucose and glucuronic acid units. The CNF gels, alone or with the addition of the water-soluble oligosaccharides, were tested on Chili pepper (Capsicum annuum). Based on the characterization of the gene expression with Next Generation Sequencing (NGS) of the C. annuum’s total messenger RNA, the differences in growth of the C. annuum seeds correlated well with the downregulation of the pathways regulating photosynthesis. A downregulation of the response to abiotic factors was detected, suggesting that these gels would improve the resistance of the C. annuum plants to abiotic stress due to, e.g., water deprivation and cold temperatures.
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| 2. |
- Johansson, Marie, 1973-, et al.
(författare)
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Building higher with light-weight timber structures - The effect of wind induced vibrations
- 2015
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Ingår i: INTER-NOISE 2015 - 44th International Congress and Exposition on Noise Control Engineering. - : The Institute of Noise Control Engineering of the USA, Inc..
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Konferensbidrag (refereegranskat)abstract
- During the last years the interest in multi-storey timber buildings has increased and several medium-to-high-rise buildings with light-weight timber structure have been designed and built. Examples of such are the 8-storey building Limnologen in Växjö, Sweden, the 9-storey Stadthaus in London, UK and being constructed at the moment, the 14-storey building Treet in Bergen, Norway. These are all light-weight and flexible structures which raise questions regarding the wind induced vibrations. For the building in Norway, the calculated vibration properties of the top floor are on the limit of being acceptable according to the ISO 101371 vibration criteria for human comfort. This paper will give a review of building systems for medium-to-high-rise timber buildings. Measured vibration properties for some medium-to-high-rise timber buildings will also be presented. These data have been used for calculating the peak acceleration values for two example buildings for comparison with the ISO standards. An analysis of the acceleration levels for a building with double the height has also been performed showing that designing for wind induced vibrations in higher timber buildings is going to be very important and that more research into this area is needed. © 2015 by ASME.
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| 3. |
- Lindstedt, Malin, et al.
(författare)
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Individuals with occupational allergy to detergent enzymes display a differential transcriptional regulation and cellular immune response
- 2005
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Ingår i: Clinical and Experimental Allergy. - : Wiley. - 0954-7894 .- 1365-2222. ; 35:2, s. 199-206
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Tidskriftsartikel (refereegranskat)abstract
- Background: In spite of significant safety measures, allergy to industrial enzymes remains a major concern. The increasing prevalence of occupational allergy emphasizes the need to investigate the functional properties of enzyme-exposed dendritic cells (DCs), as DCs possess a potent ability to activate allergen-specific T cells. Objective: This study aims at elucidating the molecular mechanisms underlying allergic immune responses to lipase, an industrial enzyme. For this purpose, we studied the effect of both hypoallergenic and wild-type lipase on the transcriptional regulation in DCs and their stimulatory effect on memory CD4+ T cells. Methods: Five individuals with documented lipase allergy were tested for specific serum IgE. DCs from these individuals, stimulated with lipases, were assayed for their ability to affect proliferation and polarization of memory T cells. The effect of lipases on transcriptional activity in DCs was evaluated using global expression analysis. Results: Lipase-specific IgE levels varied considerably between donors, with donor 4 exhibiting highest levels, and a potent specific CD4+ T cell recall response was demonstrated only for donor 4. No difference was detected in cytokine profile when T cells from donor 4 were co-cultured with DCs pulsed with either hypoallergenic or wild-type lipase, as demonstrated by high IL-4 and IL-13, and low IFN-? production. However, the lipases induced different genetic signatures in DCs from donor 4, as compared with the non-responders. Conclusions: DCs from individuals with clinically diagnosed allergy to lipase displayed a differential response to stimulation with hypoallergenic and wild-type lipase in vitro. Only allergen-pulsed DCs from donor 4 were able to induce CD4+ T cell proliferation. The lipase-specific T cells displayed a T-helper type 2 phenotype, which was not altered by hypoallergenic lipase-pulsed DCs. Furthermore, DCs derived from donor 4 and stimulated with either of the lipases displayed different transcriptional profiles, as compared with the other donors. These signatures represent genes of potential importance for an immunoregulatory role of DC in an ongoing allergic response. © 2005 Blackwell Publishing Ltd.
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| 4. |
- Mattsson, Sandra, 1984, et al.
(författare)
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Validation of the complexity index method at three manufacturing companies
- 2013
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Ingår i: Proceedings - 2013 IEEE International Symposium on Assembly and Manufacturing, ISAM 2013. - 9781479916573 ; , s. 55-57
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Konferensbidrag (refereegranskat)abstract
- In order to manage increasing numbers of product variants, tools that can reduce or manage production complexity are vital. The paper describes CompleXity Index (CXI), an index-based method and tool that assess the complexity at an industrial workstation. CXI was validated at three Swedish manufacturing companies investigating how different roles affect the index calculation and if the method measures what was intended. In all three cases, CXI was seen as a useful tool that provided a holistic view of the problems seen at a station. In addition it was indicated that complexity and unbalanced work was connected and that the method could be used to predict problem areas on new stations.
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| 5. |
- Schiött, Åsa, et al.
(författare)
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CD27- CD4+ memory T cells define a differentiated memory population at both the functional and transcriptional levels
- 2004
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Ingår i: Immunology. - : Wiley. - 0019-2805 .- 1365-2567. ; 113:3, s. 363-370
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Tidskriftsartikel (refereegranskat)abstract
- The memory T-cell population is a heterogeneous population, including both effector cells, which exert a direct secondary immune response, and resting or intermediate cells, which serve as a reservoir and exert a possible regulatory role. To further dissect the T-cell memory population residing in the CD4 +CD45RO+ T-cell pool, we studied the functional properties of memory populations identified by the CD27 marker. This marker clearly divides the memory population into two groups. One group consists of effector cells lacking CD27 and displaying a high antigen recall response. The other group consists of an intermediate memory population, displaying CD27. This latter group lacks an antigen recall response and requires costimulation for T-cell receptor triggering. To evaluate the function of the CD27+ memory pool, we analysed the transcriptional profile, using high-density microarray technology. These gene data strongly support the different functional profiles of CD27+ and CD27+ memory populations, in terms of protein expression and the capacity to respond to antigen.
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| 6. |
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