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Att välja trä
- 2020. - 10
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Samlingsverk (redaktörskap) (populärvet., debatt m.m.)
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| 3. |
- Eriksson, Kristina, et al.
(författare)
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Experiences in Running a Professional Course on Digitally-Enabled Production in Collaboration Between Three Swedish Universities
- 2022
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Ingår i: Advances in Transdisciplinary Engineering, Volume 21. - : IOS Press. - 9781614994398 ; , s. 653-664
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Konferensbidrag (refereegranskat)abstract
- Needs for new competences and knowledge arise as industry 4.0 evolves in increasingly digitalized production. This development entails that job transformations and future skills need attention from the perspective of industry 5.0, where human and machine find ways of working together to improve production performance. Facing this perspective, one challenge is a growing need for novel lifelong learning initiatives, to meet emerging and altering occupations for the fulfilment of future skill requirements. This challenge is addressed here by portraying a case where three Swedish universities have formed a distinctive collaboration to develop a flexible (i.e. blended) course for professionals, in the subject of Digitally-enabled production. The purpose is to develop a sustainable collaboration between the universities and create a course format on master level addressing lifelong learning for the increasingly digitalized production. The ambition is to increase the impact of the universities respective efforts by sharing resources and utilizing individual specialized expertise to develop a practical and relevant course that can reach a larger target group. The course encompasses industry 4.0 readiness on three levels of production systems; plant-, production cell-, and component level; to adopt a holistic view of digitalization in production. We followed an action research approach for continuously collecting and documenting our experiences during the course development, implementation, and dissemination of the course. Within the frame of action research, an explorative case study describes and analyzes the initiative. The results highlight challenges and opportunities for succeeding with this form of co-produced course. The joint course gives professionals possibilities to work on cases from their own companies with expert supervision from three manufacturing levels to address complex challenges in industry 4.0 implementation. To conclude, the importance of lifelong learning in relation to the human-centric approach of industry 5.0 is emphasized as a future direction.
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| 4. |
- Gustafsson, Ingrid, et al.
(författare)
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The nurse anesthetists' adherence to Swedish national recommendations to maintain normothermia in patients during surgery
- 2017
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Ingår i: Journal of Perianesthesia Nursing. - : Elsevier. - 1089-9472 .- 1532-8473. ; 32:5, s. 409-418
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Tidskriftsartikel (refereegranskat)abstract
- PurposeThe aim of this study was to determine if nurse anesthetists (NAs) have access, knowledge, and adhere to recommended guidelines to maintain normal body temperature during the perioperative period.DesignA descriptive survey design.MethodsQuestionnaires were sent to heads of the department (n = 56) and NAs in the operating departments in Sweden.FindingThe level of access to the recommendations is high, but only one third of the operating departments have included the recommendations in their own local guidelines. The NAs' adherence was low, between 5% and 67%, and their knowledge levels were 57% to 60%.ConclusionsA high level of knowledge, access, and adherence are important for the organization of operating departments to prevent barriers against implementation of new recommendations or guidelines. There are needs for education about patients' heat loss due to redistribution and clear recommendations.
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- Haataja, Topi, et al.
(författare)
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Enzyme kinetics by GH7 cellobiohydrolases on chromogenic substrates is dictated by non-productive binding : insights from crystal structures and MD simulation
- 2023
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Ingår i: The FEBS Journal. - : John Wiley & Sons. - 1742-464X .- 1742-4658. ; 290:2, s. 379-399
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Tidskriftsartikel (refereegranskat)abstract
- Cellobiohydrolases (CBHs) in the glycoside hydrolase family 7 (GH7) (EC3.2.1.176) are the major cellulose degrading enzymes both in industrial settings and in the context of carbon cycling in nature. Small carbohydrate conjugates such as p-nitrophenyl-beta-d-cellobioside (pNPC), p-nitrophenyl-beta-d-lactoside (pNPL) and methylumbelliferyl-beta-d-cellobioside have commonly been used in colorimetric and fluorometric assays for analysing activity of these enzymes. Despite the similar nature of these compounds the kinetics of their enzymatic hydrolysis vary greatly between the different compounds as well as among different enzymes within the GH7 family. Through enzyme kinetics, crystallographic structure determination, molecular dynamics simulations, and fluorometric binding studies using the closely related compound o-nitrophenyl-beta-d-cellobioside (oNPC), in this work we examine the different hydrolysis characteristics of these compounds on two model enzymes of this class, TrCel7A from Trichoderma reesei and PcCel7D from Phanerochaete chrysosporium. Protein crystal structures of the E212Q mutant of TrCel7A with pNPC and pNPL, and the wildtype TrCel7A with oNPC, reveal that non-productive binding at the product site is the dominating binding mode for these compounds. Enzyme kinetics results suggest the strength of non-productive binding is a key determinant for the activity characteristics on these substrates, with PcCel7D consistently showing higher turnover rates (k(cat)) than TrCel7A, but higher Michaelis-Menten (K-M) constants as well. Furthermore, oNPC turned out to be useful as an active-site probe for fluorometric determination of the dissociation constant for cellobiose on TrCel7A but could not be utilized for the same purpose on PcCel7D, likely due to strong binding to an unknown site outside the active site.
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- Hu, Yue O. O., et al.
(författare)
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Stationary and portable sequencing-based approaches for tracing wastewater contamination in urban stormwater systems
- 2018
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Ingår i: Scientific Reports. - : Nature Publishing Group. - 2045-2322. ; 8
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Tidskriftsartikel (refereegranskat)abstract
- Urban sewer systems consist of wastewater and stormwater sewers, of which only wastewater is processed before being discharged. Occasionally, misconnections or damages in the network occur, resulting in untreated wastewater entering natural water bodies via the stormwater system. Cultivation of faecal indicator bacteria (e.g. Escherichia coli; E. coli) is the current standard for tracing wastewater contamination. This method is cheap but has limited specificity and mobility. Here, we compared the E. coli culturing approach with two sequencing-based methodologies (Illumina MiSeq 16S rRNA gene amplicon sequencing and Oxford Nanopore MinION shotgun metagenomic sequencing), analysing 73 stormwater samples collected in Stockholm. High correlations were obtained between E. coli culturing counts and frequencies of human gut microbiome amplicon sequences, indicating E. coli is indeed a good indicator of faecal contamination. However, the amplicon data further holds information on contamination source or alternatively how much time has elapsed since the faecal matter has entered the system. Shotgun metagenomic sequencing on a subset of the samples using a portable real-time sequencer, MinION, correlated well with the amplicon sequencing data. This study demonstrates the use of DNA sequencing to detect human faecal contamination in stormwater systems and the potential of tracing faecal contamination directly in the field.
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| 7. |
- Johansson, Lotta, 1965, et al.
(författare)
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Evaluation of a sound environment intervention in an ICU : A feasibility study
- 2018
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Ingår i: Australian Critical Care. - Amsterdam : Elsevier. - 1036-7314 .- 1878-1721. ; 31:2, s. 59-70
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Tidskriftsartikel (refereegranskat)abstract
- Background: Currently, it is well known that the sound environment in intensive care units (ICU) is substandard. Therefore, there is a need of interventions investigating possible improvements. Unfortunately, there are many challenges to consider in the design and performance of clinical intervention studies including sound measurements and clinical outcomes.Objectives: (1) explore whether it is possible to implement a full-scale intervention study in the ICU concerning sound levels and their impact on the development of ICU delirium; (2) discuss methodological challenges and solutions for the forthcoming study; (3) conduct an analysis of the presence of ICU delirium in the study group; and (4) describe the sound pattern in the intervention rooms.Methods: A quasi-randomized clinical trial design was chosen. The intervention consisted of a refurbished two-bed ICU patient room (experimental) with a new suspended wall-to-wall ceiling and a low frequency absorber. An identical two-bed room (control) remained unchanged.Inclusion criteria: Patients >18 years old with ICU lengths of stay (LoS) >48. h. The final study group consisted of 31 patients: six from the rebuilt experimental room and 25 from the control room. Methodological problems and possible solutions were continuously identified and documented.Results: Undertaking a full-scale intervention study with continuous measurements of acoustic data in an ICU is possible. However, this feasibility study demonstrated some aspects to consider before start. The randomization process and the sound measurement procedure must be developed. Furthermore, proper education and training are needed for determining ICU delirium.Conclusion: This study raises a number of points that may be helpful for future complex interventions in an ICU. For a full-scale study to be completed a continuously updated cost calculation is necessary. Furthermore, representatives from the clinic need to be involved in all stages during the project.
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| 8. |
- Johansson, Victoria, et al.
(författare)
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Språk och hjärna
- 2013. - 1
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Ingår i: Språket, människan och världen. - Lund : Studentlitteratur AB. - 9789144083391 ; , s. 225-241
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 9. |
- Jonna, Venkateswara Rao, et al.
(författare)
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Diversity in Overall Activity Regulation of Ribonucleotide Reductase
- 2015
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Ingår i: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; , s. 1-24
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Tidskriftsartikel (refereegranskat)abstract
- Ribonucleotide reductase (RNR) catalyzes the reduction of ribonucleotides to the corresponding deoxyribonucleotides, which are used as building blocks for DNA replication and repair. This process is tightly regulated via two allosteric sites, the specificity site (s-site) and the overall activity site (a-site). The a-site resides in an N-terminal ATP cone domain that binds dATP or ATP and functions as an on/off switch, whereas the composite s-site binds ATP, dATP, dTTP, or dGTP and determines which substrate to reduce. There are three classes of RNRs, and class I RNRs consist of different combinations of α and β subunits. In eukaryotic and Escherichia coli canonical class I RNRs, dATP inhibits enzyme activity through the formation of inactive α6 and α4β4 complexes, respectively. Here we show that the Pseudomonas aeruginosa class I RNR has a duplicated ATP cone domain and represents a third mechanism of overall activity regulation. Each α polypeptide binds three dATP molecules, and the N-terminal ATP cone is critical for binding two of the dATPs because a truncated protein lacking this cone could only bind one dATP to its s-site. ATP activates the enzyme solely by preventing dATP from binding. The dATP-induced inactive form is an α4 complex, which can interact with β2 to form a non-productive α4β2 complex. Other allosteric effectors induce a mixture of α2 and α4 forms, with the former being able to interact with β2 to form active α2β2 complexes. The unique features of the P. aeruginosa RNR are interesting both from evolutionary and drug discovery perspectives.
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