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- Blacque, O E, et al.
(författare)
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Functional genomics of the cilium, a sensory organelle
- 2005
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Ingår i: Current Biology. - : Elsevier BV. - 0960-9822 .- 1879-0445. ; 15:10, s. 935-941
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Tidskriftsartikel (refereegranskat)abstract
- Cilia and flagella play important roles in many physiological processes, including cell and fluid movement, sensory perception, and development [1]. The biogenesis and maintenance of cilia depend on intraflagellar transport (IFT), a motility process that operates bidirectionally along the ciliary axoneme [1, 2]. Disruption in IFT and cilia function causes several human disorders, including polycystic kidneys, retinal dystrophy, neurosensory impairment, and Bardet-Bledl syndrome (BBS) [3-5]. To uncover new ciliary components, including IFT proteins, we compared C. elegans ciliated neuronal and nonciliated cells through serial analysis of gene expression (SAGE) and screened for genes potentially regulated by the cillogenic transcription factor, DAF-19 [6]. Using these complementary approaches, we identified numerous candidate ciliary genes and confirmed the ciliated-cell-specific expression of 14 novel genes. One of these, C27H5.7a, encodes a ciliary protein that undergoes IFT. As with other IFT proteins, its ciliary localization and transport is disrupted by mutations in IFT and bbs genes. Furthermore, we demonstrate that the ciliary structural defect of C. elegans dyf-13(mn396) mutants is caused by a mutation in C27H5.7a. Together, our findings help define a ciliary transcriptome and suggest that DYF-13, an evolutionarily conserved protein, is a novel core IFT component required for cilia function.
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- Andrén, Elinor, et al.
(författare)
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The relative influences of climate and volcanic activity on Holocene lake development inferred from a mountain lake in central Kamchatka
- 2015
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Ingår i: Global and Planetary Change. - : Elsevier BV. - 1872-6364 .- 0921-8181. ; 134, s. 67-81
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Tidskriftsartikel (refereegranskat)abstract
- A sediment sequence was taken from a closed, high altitude lake (informal name Olive-backed Lake) in the central mountain range of Kamchatka, in the Russian Far East. The sequence was dated by radiocarbon and tephrochronology and used for multi-proxy analyses (chironomids, pollen, diatoms). Although the evolution of Beringian climate through the Holocene is primarily driven by global forcing mechanisms, regional controls, such as volcanic activity or vegetation dynamics, lead to a spatial heterogeneous response. This study aims to reconstruct past changes in the aquatic and terrestrial ecosystems and to separate the climate-driven response from a response to regional or localised environmental change. Radiocarbon dates from plant macrophytes gave a basal date of 7800 cal yr BP. Coring terminated in a tephra layer, so sedimentation at the lake started prior to this date, possibly in the early Holocene following local glacier retreat. Initially the catchment vegetation was dominated by Betula and Alnus woodland with a mosaic of open, wet, aquatic and semi-aquatic habitats. Between 7800 and 6000 cal yr BP the diatom-inferred lake water was pH 4.4-53 and chironomid and diatom assemblages in the lake were initially dominated by a small number of acidophilic/acid tolerant taxa. The frequency of Pinus pumila (Siberian dwarf pine) pollen increased from 5000 cal yr BP and threshold analysis indicates that P. pumila arrived in the catchment between 4200 and 3000 cal yr BP. Its range expansion was probably mediated by strengthening of the Aleutian Low pressure system and increased winter snowfall. The diatominferred pH reconstructions show that after an initial period of low pH, pH gradually increased from 5500 cal yr BP to pH 5.8 at 1500 cal yr BP. This trend of increasing pH through the Holocene is unusual in lake records, but the initially low pH may have resulted directly or indirectly from intense regional volcanic activity during the midHolocene. The chironomid-inferred July temperature reconstruction suggests cool periods between 32002800 cal yr BP and 1100-700 cal yr BP and a warmer period between 2800 and 1100 cal yr BP. Chironomid and diatom DCA scores decline from ca. 6000 cal yr BP, indicating compositional changes in these aquatic assemblages. In comparison declines in pollen PCA scores are delayed, starting ca. 5100 cal yr BP. The results suggest that while catchment vegetation was responding primarily to climate change, the biota within the lake and lake water chemistry were responding to localised environmental conditions. Crown Copyright (C) 2015 Published by Elsevier B.V. All rights reserved.
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