| 1. |
|
|
| 2. |
- Bräutigam, Marcus, 1968, et al.
(författare)
-
Development of Swedish winter oat with gene technology and molecular breeding
- 2006
-
Ingår i: J. Seed Science. - 0039-6990. ; 116:1-2, s. 12-35
-
Tidskriftsartikel (refereegranskat)abstract
- In Sweden, oat (Avena sativa) is only grown as a spring crop. A Swedish winter oat, on the other hand, would give increased yields and would secure oat in Swedish agriculture. During three consecutive winters we performed field trials with oat aiming at identifying potential winter material. More than 300 varieties, originating from breeding programs all over the world, were tested. Plants were rated according to winter survival, vigour and general performance during the following growth season and more than 20 lines were identified that were cold hardier than present commercial oat varieties. In parallel experiments a cDNA library was constructed from cold induced English winter oat (Gerald) and ca 10000 EST sequences were generated. After data mining a UniGene set of 2800 oat genes was obtained. By detailed analysis of microarray data from cold stressed Arabidopsis and by advanced bioinformatics, gene interactions in the complex cold induced signal transduction pathway were deduced. By comparison to the oat UniGene set, several genes potentially involved in the regulation of cold hardiness in oat were identified. An Agrobacterium mediated transformation protocol was developed for one oat genotype. Key regulatory genes in cold acclimation will be introduced to oat by genetic transformation or modified by TILLING. Such genes will be used as molecular markers in intogression of winter hardiness to commercial oat.
|
|
| 3. |
|
|
| 4. |
- Glimskär, Anders, et al.
(författare)
-
Inventering och utveckling för uppföljning av gräsmarks- och hällmarksnaturtyper 2017
- 2018
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Rapporten beskriver resultat från biogeografisk uppföljning av några naturtyper med sparsam förekomst i landskapet. För hällmarkstorrängar (naturtyp 8230) visas resultat från tredje året av uppföljningen, som bland annat visar att en stor andel av hällmarkstorrängarna ligger utanför skyddade områden. För alvar, basiska berghällar och karsthällmark är 2017 det första året för ett förtätat stickprov på Öland och Gotland. Förutom variabler för artförekomst och vegetationsstruktur ingår också beskrivning av vittringsmaterial och karstsprickor samt en särskild metodik för karstlevande snäckor. Svämängar har karterats i sin helhet längs Vindelälven, med ett stickprov av fältbesökta ytor. De kommande fem åren är planen att svämängar vid samtliga större vattendrag från Dalälven och norrut karteras. Förutom uppföljningen av dessa naturtyper, så har även utvecklingsinsatser gjorts för andra naturtyper, som underlag för framtida uppföljning. Ett mindre antal objekt av höglänta slåtterängar och slåtterängar i låglandet har inventerats, för att ge underlag för hur artsammansättningen kan ge stöd för en tillförlitlig klassning av slåtter- och betespräglade naturtyper. Första tester har gjorts för hur högörtängar och svämängar kan följas vid mindre vattendrag, men fler år behövs för att slutsatserna ska bli tydliga. Tester har också gjorts för hur Remiils miljöövervakning i gräsmarker kan bidra för vanliga gräsmarksnaturtyper, men en bra lösning förutsätter kraftigt förbättrad samordning mellan olika inventeringar.
|
|
| 5. |
- Herlitz, Johan, et al.
(författare)
-
Suspicion and treatment of severe sepsis : An overview of the prehospital chain of care
- 2012
-
Ingår i: Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine. - : BioMed Central. - 1757-7241. ; 20:42
-
Tidskriftsartikel (refereegranskat)abstract
- BackgroundSepsis is a life-threatening condition where the risk of death has been reported to be even higher than that associated with the major complications of atherosclerosis, i.e. myocardial infarction and stroke. In all three conditions, early treatment could limit organ dysfunction and thereby improve the prognosis.AimTo describe what has been published in the literature a/ with regard to the association between delay until start of treatment and outcome in sepsis with the emphasis on the pre-hospital phase and b/ to present published data and the opportunity to improve various links in the pre-hospital chain of care in sepsis.MethodsA literature search was performed on the PubMed, Embase (Ovid SP) and Cochrane Library databases.ResultsIn overall terms, we found a small number of articles (n=12 of 1,162 unique hits) which addressed the prehospital phase. For each hour of delay until the start of antibiotics, the prognosis appeared to become worse. However, there was no evidence that prehospital treatment improved the prognosis.Studies indicated that about half of the patients with severe sepsis used the emergency medical service (EMS) for transport to hospital. Patients who used the EMS experienced a shorter delay to treatment with antibiotics and the start of early goal-directed therapy (EGDT). Among EMS-transported patients, those in whom the EMS staff already suspected sepsis at the scene had a shorter delay to treatment with antibiotics and the start of EGDT.There are insufficient data on other links in the prehospital chain of care, i.e. patients, bystanders and dispatchers.ConclusionSevere sepsis is a life-threatening condition. Previous studies suggest that, with every hour of delay until the start of antibiotics, the prognosis deteriorates. About half of the patients use the EMS. We need to know more about the present situation with regard to the different links in the prehospital chain of care in sepsis.
|
|
| 6. |
|
|
| 7. |
- Lindmark, Fredrik, et al.
(författare)
-
Analysis of the macrophage scavenger receptor 1 gene in Swedish hereditary and sporadic prostate cancer.
- 2004
-
Ingår i: The Prostate. - : Wiley. - 0270-4137 .- 1097-0045. ; 59:2, s. 132-140
-
Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: The macrophage scavenger receptor 1 (MSR1) gene on chromosome 8p22 was recently reported as a candidate gene for hereditary prostate cancer (HPC). Here, we further elucidate the role of MSR1 in both Swedish families with HPC and in a cohort of unselected prostate cancer. METHODS: DNA samples from 83 Swedish HPC families and 215 unselected population based cases of prostate cancer as well as 425 age-matched controls were genotyped. RESULTS: A total of 18 variants were identified, including 2 exonic, 7 intronic changes, and 9 changes in the 5'- or 3'-uncoding region. Of the two exonic changes, one previously reported truncation mutation was identified, a R293X nonsense mutation. This mutation was found in 2 of the 83 (2.4%) HPC families. The R293X mutation was found more frequently in men with PC (4.9%) than in unaffected men (2.7%), consistent with previous published results, however our results were not significant (P = 0.16). To additionally test for potential association of common sequence variants and increased risk for the disease, five common polymorphisms (PRO3, INDEL1, IVS5-57, P275A, INDEL7) were genotyped in the group of 215 prostate cancer cases and 425 age-matched controls. No association between any of the five common sequence variants and prostate cancer were found. CONCLUSION: Our results suggest that mutations in MSR1 gene might play a role in prostate cancer susceptibility, particularly the R293X mutation. This study warrants further investigations of the role of MSR1 in prostate cancer etiology.
|
|
| 8. |
- Stattin, Eva-Lena, et al.
(författare)
-
A missense mutation in the aggrecan C-type lectin domain disrupts extracellular matrix interactions and causes dominant familial osteochondritis dissecans
- 2010
-
Ingår i: American Journal of Human Genetics. - : Elsevier BV. - 0002-9297 .- 1537-6605. ; 86:2, s. 126-137
-
Tidskriftsartikel (refereegranskat)abstract
- Osteochondritis dissecans is a disorder in which fragments of articular cartilage and subchondral bone dislodge from the joint surface. We analyzed a five-generation family in which affected members had autosomal-dominant familial osteochondritis dissecans. A genome-wide linkage analysis identified aggrecan (ACAN) as a prime candidate gene for the disorder. Sequence analysis of ACAN revealed heterozygosity for a missense mutation (c.6907G > A) in affected individuals, resulting in a p.V2303M amino acid substitution in the aggrecan G3 domain C-type lectin, which mediates interactions with other proteins in the cartilage extracellular matrix. Binding studies with recombinant mutated and wild-type G3 proteins showed loss of fibulin-1, fibulin-2, and tenascin-R interactions for the V2303M protein. Mass spectrometric analyses of aggrecan purified from patient cartilage verified that V2303M aggrecan is produced and present in the tissue. Our results provide a molecular mechanism for the etiology of familial osteochondritis dissecans and show the importance of the aggrecan C-type lectin interactions for cartilage function in vivo.
|
|
| 9. |
|
|
| 10. |
- Suserud, Björn-Ove, et al.
(författare)
-
Caring for patients at high speed
- 2013
-
Ingår i: Emergency Nurse. - : RCNI. - 1354-5752 .- 2047-8984. ; 21:7, s. 14-18
-
Tidskriftsartikel (refereegranskat)abstract
- Aim: The aim of this article is to explore whether ambulance clinicians in Sweden perceive their working environment to be safe. Method: Twenty four ambulance nurses and nine paramedics at five ambulance stations in urban and rural areas of Sweden were interviewed. Findings: After transcripts of the interviews had been analysed, nine issues that affect how participants perceive the safety of patient care in ambulances emerged: planning before departure; use of safety belts; driving at high speeds; patient first, safety second; equipment design and placement; noise; driving styles; presence of relatives; documentation. Conclusion: Ambulance personnel should have greater involvement in the design of ambulance care spaces and drivers should be given more regular training.
|
|
