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> Watanabe M. >
Comparative anatomi...
Comparative anatomical distribution of neuronal calcium-binding protein (NECAB) 1 and -2 in rodent and human spinal cord
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- Zhang, M. -D (author)
- Karolinska Institutet
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- Barde, S. (author)
- Karolinska Institutet
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Szodorai, E. (author)
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Josephson, A. (author)
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- Mitsios, N. (author)
- Karolinska Institutet
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Watanabe, M. (author)
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Attems, J. (author)
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Lubec, G. (author)
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Kovács, G. M. (author)
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- Uhlén, Mathias (author)
- KTH,Proteomik och nanobioteknologi
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- Mulder, J. (author)
- Karolinska Institutet
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- Harkany, T. (author)
- Karolinska Institutet
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- Hökfelt, T. (author)
- Karolinska Institutet
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Kovacs, GG (author)
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(creator_code:org_t)
- 2016-02-02
- 2016
- English.
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In: Brain Structure and Function. - : Springer. - 1863-2653 .- 1863-2661. ; , s. 1-21
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Subject headings
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- Neuronal calcium-binding protein 1 and -2 (NECAB1/2) localize to multiple excitatory neuron populations in the mouse spinal cord. Here, we analyzed rat and human spinal cord, combining in situ hybridization and immunohistochemistry, complementing newly collated data on mouse spinal cord for direct comparisons. Necab1/2 mRNA transcripts showed complementary distribution in rodent’s spinal cord. Multiple-labeling fluorescence histochemistry with neuronal phenotypic markers localized NECAB1 to a dense fiber plexus in the dorsal horn, to neurons mainly in superficial layers and to commissural interneurons in both rodent species. NECAB1-positive (+) motor neurons were only found in mice. NECAB1 distribution in the human spinal cord was similar with the addition of NECAB1-like immunoreactivity surrounding myelinated axons. NECAB2 was mainly present in excitatory synaptic boutons in the dorsal horn of all three species, and often in calbindin-D28k+ neuronal somata. Rodent ependymal cells expressed calbindin-D28k. In humans, they instead were NECAB2+ and/or calretinin+. Our results reveal that the association of NECAB2 to excitatory neuronal circuits in the spinal cord is evolutionarily conserved across the mammalian species investigated so far. In contrast, NECAB1 expression is more heterogeneous. Thus, our study suggests that the phenotypic segregation of NECAB1 and -2 to respective excitatory and inhibitory spinal systems can underpin functional modalities in determining the fidelity of synaptic neurotransmission and neuronal responsiveness, and might bear translational relevance to humans.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- Calbindin-D28k
- Ependymal cell
- PKCγ
- SST2A
- VGLUT1
- VGLUT2
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Zhang, M. -D
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Barde, S.
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Szodorai, E.
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Josephson, A.
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Mitsios, N.
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Watanabe, M.
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show more...
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Attems, J.
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Lubec, G.
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Kovács, G. M.
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Uhlén, Mathias
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Mulder, J.
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Harkany, T.
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Hökfelt, T.
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Kovacs, GG
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
- Articles in the publication
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Brain Structure ...
- By the university
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Royal Institute of Technology
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Karolinska Institutet