1. |
- Andersson, Erik, et al.
(författare)
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Ambio fit for the 2020s
- 2022
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Ingår i: Ambio. - : Springer Nature. - 0044-7447 .- 1654-7209. ; 51:5, s. 1091-1093
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Tidskriftsartikel (refereegranskat)
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2. |
- Kalantari, Aida, 1986, et al.
(författare)
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Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis
- 2017
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Ingår i: Frontiers in Microbiology. - : Frontiers Media SA. - 1664-302X. ; 8:APR
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Tidskriftsartikel (refereegranskat)abstract
- 3-Hydroxypropanoic acid (3-HP) is an important biomass-derivable platform chemical that can be converted into a number of industrially relevant compounds. There have been several attempts to produce 3-HP from renewable sources in cell factories, focusing mainly on Escherichia coli, Klebsiella pneumoniae, and Saccharomyces cerevisiae. Despite the significant progress made in this field, commercially exploitable large-scale production of 3-HP in microbial strains has still not been achieved. In this study, we investigated the potential of Bacillus subtilis as a microbial platform for bioconversion of glycerol into 3-HP. Our recombinant B. subtilis strains overexpress the two-step heterologous pathway containing glycerol dehydratase and aldehyde dehydrogenase from K. pneumoniae. Genetic engineering, driven by in silico optimization, and optimization of cultivation conditions resulted in a 3-HP titer of 10 g/L, in a standard batch cultivation. Our findings provide the first report of successful introduction of the biosynthetic pathway for conversion of glycerol into 3-HP in B. subtilis. With this relatively high titer in batch, and the robustness of B. subtilis in high density fermentation conditions, we expect that our production strains may constitute a solid basis for commercial production of 3-HP.
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3. |
- Khandani, Arefeh Kalantari, et al.
(författare)
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Millimeter wave Wideband and Low-loss Compact Power Divider Based on Gap Waveguide: For Use in Wideband Antenna Array System
- 2024
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Ingår i: IEEE Access. - 2169-3536 .- 2169-3536. ; 12, s. 116478-116488
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Tidskriftsartikel (refereegranskat)abstract
- This paper presents a wideband and low-loss design of a compact power divider based on gap waveguide technology. The proposed power divider consists of two adjacent E-plane groove gap waveguide and a small ridge section to couple and equally divide the EM energy from the input E-plane groove gap waveguide to the two output ones in-phase. The simulation results show that the proposed waveguide power divider has about 40% impedance bandwidth while its size is 0.7λ × 0.6λ at the center frequency. An 8-way power divider is designed using the proposed 2-way power divider and the back-toback configuration of the 8-way one is fabricated to investigate the performance of the proposed design. In addition, to show the application of the presented power divider, a wideband linear horn antenna array is designed and fabricated. The measured results agree well with the simulated ones and prove the excellent low-loss and wide bandwidth of the proposed power divider over the band of interest from 50-75GHz. The measured S 11 of the entire 8-way power divider remains below -10 dB level, the insertion loss is around 1 dB over the band of interest. Also, the S 11 of the horn array integrated with the 8-way feed network remains below -10 dB and the low sidelobes of the radiation pattern of the entire horn array indicates excellent phase and amplitude balance for the power divider over the entire bandwidth of interest.
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