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- Abrahamsson, L., et al.
(författare)
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Behöver arbetslinjen inte forskning?
- 2007
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Ingår i: Svenska Dagbladet Brännpunkt (debattinlägg). ; :2007-11-28
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 5. |
- Karlsson, J, et al.
(författare)
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Roles of Individual Amino Acids in Helix 14 of the Membrane Domain of Proton-Translocating Transhydrogenase from Escherichia coli As Deduced from Cysteine Mutagenesis
- 2003
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Ingår i: Biochemistry. - : American Chemical Society (ACS). - 0006-2960 .- 1520-4995. ; 42, s. 6575-6581
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Tidskriftsartikel (refereegranskat)abstract
- Proton-translocating nicotinamide nucleotide transhydrogenase is a membrane-bound protein composed of three domains: the hydrophilic NAD(H)-binding domain, the hydrophilic NADP(H)-binding domain, and the hydrophobic membrane domain. The latter harbors the proton channel. In Escherichia coli transhydrogenase, the membrane domain is composed of 13 transmembrane helices, of which especially helices 13 and 14 contain conserved residues. To characterize the roles of the individual residues Leu240 to Ser260 in helix 14, these were mutated as single mutants to cysteines in the cysteine-free background, and in the case of Gly245, Gly249, and Gly252, also to leucines. In addition to the residues forming the helix, residues Asn238 and Asp239 were also mutated. Except for I242C, all mutants were normally expressed, purified, and characterized with respect to, e.g., catalytic activities and proton pumping. The results show that mutation of the conserved glycines Gly245, Gly249, and Gly252, located on the same face of the helix, led to a general inhibition of all activities, especially in the case of Gly252, suggesting a role of these glycines in helix-helix interactions. In contrast, mutation of the conserved serines Ser250, Ser251, and Ser256 led to enhanced activities of all reactions, including the cyclic reaction which was mediated by bound NADP(H). Mutation of the remaining residues resulted in intermediate inhibitory effects. The results strongly support an important regulatory role of the membrane domain on the NADP(H)-binding site.
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| 6. |
- Lexell, Jan, et al.
(författare)
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Isokinetic torque and surface electromyography during fatiguing muscle contractions in young and older men and women
- 2006
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Ingår i: Isokinetics and exercise science. - 0959-3020 .- 1878-5913. ; 14:3, s. 225-234
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Tidskriftsartikel (refereegranskat)abstract
- The overall aim was to investigate the fatigue pattern and perceived fatigue during repetitive dynamic contractions in young and older men and women. Sixteen older men and women (mean age 73 ± 3 years) and 22 young men and women (mean age 28 ± 6 years) performed 100 repeated maximum concentric isokinetic knee extension contractions at 90°·s-1 (1.57 rad·s-1). Throughout the 100 contractions, peak torque (PT) and surface electromyography (EMG) signals from the rectus femoris muscle and the vastus lateralis muscle were recorded simultaneously, together with perceived fatigue (CR-10 scale of Borg). The mean frequency of the power spectrum (MNF) and the root mean square of the signal amplitude (RMS) of the EMG were affected by age and with a sex related difference but for PT the difference of the fatigue pattern was most evident between the sexes. In addition, the young men rated the highest fatigue. Based on EMG, PT and ratings of perceived fatigue it was concluded that the old men fatigued most, the old women fatigued least and the young men perceived greatest fatigue.
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| 8. |
- Nygårdh, Annette, et al.
(författare)
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An experience-based co-design to accomplish person-centered self-care support for elderly persons with heart failure
- 2015
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Ingår i: European Journal of Cardiovascular Nursing. - 1474-5151 .- 1873-1953. ; 14, s. 51-52
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Tidskriftsartikel (refereegranskat)abstract
- Introduction: Self-care refers to activities performed with the intention of improving or restoring health and well-being. A good understanding of the adaptations needed in daily life is a prerequisite to motivate individuals to implement such changes. Facilitating the person with heart-failure’s (HF) understanding of the diagnosis, how to handle symptoms, and when to seek healthcare, is one way of proactive self-care. Previous Information and communication technology (ICT) support has proved to be effective in younger patients, but the development has not been user-driven, i.e. involving references and experiences from the patient, their family members or healthcare professionals and have rarely been implemented in clinical practice. In addition, disregard of the user hands over control and responsibility to the healthcare professionals and misses the patient’s desires and requirements.Aim: To describe an experience-based co-design of ICT for self-care support in the context of heart failure careMethods: The steps in the process of developing ICT support programs for self-care were guided by an experienced based co-design methodology including: 1) Workshops regarding self-care needs. 2) Workshops regarding self-care support as a means to cope with the phases of the disease. 3) Workshops to assess the usability of the suggested ideas. 4) Workshops to test the different supports in action. The project involved older persons with HF, their family members, healthcare professionals, IT-developers, informatics, development leaders, and researchers. There was also a quality improvement process involved to improve working routines and communication between the person with HF and the healthcare professionals, which in turn, increases the value of the innovation. Data collection include tape- and video recorded workshops and diary notes supplied by the informants. All data are to be analyzed by content analysis to identify needs, opportunities and challenges of self-care.Results and conclusion: This design was found to be a useful method for finding and assess usability of person-centered self-care ICT support. To involve users in the development of self-care support has improved the opportunities to realize support needed. Sharing these perspectives increases professionally and organizationally learning in order to accomplish person-centered self-care. Preliminary findings show that ICT will be a part of solving some of the self-care needs described by patients, family member and health care providers and the next step in the project will be to test and adapt these tools.
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| 9. |
- Pedersen, Anders, 1976, et al.
(författare)
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Properties of the apo-form of the NADP(H)-binding domain III of proton-pumping Escherichia coli transhydrogenase: implications for the reaction mechanism of the intact enzyme
- 2003
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Ingår i: Biochim.Biophys. Acta (Bioenergetics). ; 1604, s. 55-59
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Tidskriftsartikel (refereegranskat)abstract
- Proton-translocating nicotinamide nucleotide transhydrogenases contain an NAD(H)-binding domain (dI), an NADP(H)-binding domain (dIII) and a membrane domain (dII) with the proton channel. Separately expressed and isolated dIII contains tightly bound NADP(H), predominantly in the oxidized form, possibly representing a so-called “occluded” intermediary state of the reaction cycle of the intact enzyme. Despite a Kd in the micromolar to nanomolar range, this NADP(H) exchanges significantly with the bulk medium. Dissociated NADP+ is thus accessible to added enzymes, such as NADP-isocitrate dehydrogenase, and can be reduced to NADPH. In the present investigation, dissociated NADP(H) was digested with alkaline phosphatase, removing the 2′-phosphate and generating NAD(H). Surprisingly, in the presence of dI, the resulting NADP(H)-free dIII catalyzed a rapid reduction of 3-acetylpyridine-NAD+ by NADH, indicating that 3-acetylpyridine-NAD+ and/or NADH interacts unspecifically with the NADP(H)-binding site. The corresponding reaction in the intact enzyme is not associated with proton pumping. It is concluded that there is a 2′-phosphate-binding region in dIII that controls tight binding of NADP(H) to dIII, which is not a required for fast hydride transfer. It is likely that this region is the Lys424–Arg425–Ser426 sequence and loops D and E. Further, in the intact enzyme, it is proposed that the same region/loops may be involved in the regulation of NADP(H) binding by an electrochemical proton gradent.
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| 10. |
- TIGERSTRÖM, ANNA KATARINA, 1976, et al.
(författare)
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Functional split and crosslinking of the membrane domain of the beta subunit of proton-translocating transhydrogenase from Escherichia coli
- 2003
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Ingår i: Biochemistry. - : American Chemical Society (ACS). - 0006-2960 .- 1520-4995. ; 42, s. 10998-11003
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Tidskriftsartikel (refereegranskat)abstract
- Proton pumping nicotinamide nucleotide transhydrogenase from Escherichia coli contains an alpha subunit with the NAD(H)-binding domain I and a beta subunit with the NADP(H)-binding domain III. The membrane domain (domain II) harbors the proton channel and is made up of the hydrophobic parts of the alpha and beta subunits. The interface in domain II between the alpha and the beta subunits has previously been investigated by cross-linking loops connecting the four transmembrane helices in the alpha subunit and loops connecting the nine transmembrane helices in the beta subunit. However, to investigate the organization of the nine transmembrane helices in the beta subunit, a split was introduced by creating a stop codon in the loop connecting transmembrane helices 9 and 10 by a single mutagenesis step, utilizing an existing downstream start codon. The resulting enzyme was composed of the wild-type alpha subunit and the two new peptides beta1 and beta2. As compared to other split membrane proteins, the new transhydrogenase was remarkably active and catalyzed activities for the reduction of 3-acetylpyridine-NAD(+) by NADPH, the cyclic reduction of 3-acetylpyridine-NAD(+) by NADH (mediated by bound NADP(H)), and proton pumping, amounting to about 50-107% of the corresponding wild-type activities. These high activities suggest that the alpha subunit was normally folded, followed by a concerted folding of beta1 + beta2. Cross-linking of a betaS105C-betaS237C double cysteine mutant in the functional split cysteine-free background, followed by SDS-PAGE analysis, showed that helices 9, 13, and 14 were in close proximity. This is the first time that cross-linking between helices in the same beta subunit has been demonstrated.
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