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Sökning: WFRF:(Karlsson Linda) > Naturvetenskap

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2.
  • Ekstrand, Eva-Maria, 1985-, et al. (författare)
  • Identifying targets for increased biogasproduction through chemical and organicmatter characterization of digestate from full‑scale biogas plants : what remains and why?
  • 2022
  • Ingår i: Biotechnology for Biofuels and Bioproducts. - London, United Kingdom : BioMed Central. - 2731-3654. ; 15:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: This study examines the destiny of macromolecules in different full-scale biogas processes. From previousstudies it is clear that the residual organic matter in outgoing digestates can have significant biogas potential,but the factors dictating the size and composition of this residual fraction and how they correlate with the residualmethane potential (RMP) are not fully understood. The aim of this study was to generate additional knowledge of thecomposition of residual digestate fractions and to understand how they correlate with various operational and chemicalparameters. The organic composition of both the substrates and digestates from nine biogas plants operating onfood waste, sewage sludge, or agricultural waste was characterized and the residual organic fractions were linked tosubstrate type, trace metal content, ammonia concentration, operational parameters, RMP, and enzyme activity.Results: Carbohydrates represented the largest fraction of the total VS (32–68%) in most substrates. However, inthe digestates protein was instead the most abundant residual macromolecule in almost all plants (3–21 g/kg). Thedegradation efficiency of proteins generally lower (28–79%) compared to carbohydrates (67–94%) and fats (86–91%).High residual protein content was coupled to recalcitrant protein fractions and microbial biomass, either from thesubstrate or formed in the degradation process. Co-digesting sewage sludge with fat increased the protein degradationefficiency with 18%, possibly through a priming mechanism where addition of easily degradable substrates alsotriggers the degradation of more complex fractions. In this study, high residual methane production (> 140 L CH4/kgVS) was firstly coupled to operation at unstable process conditions caused mainly by ammonia inhibition (0.74 mgNH3-N/kg) and/or trace element deficiency and, secondly, to short hydraulic retention time (HRT) (55 days) relative tothe slow digestion of agricultural waste and manure.Conclusions: Operation at unstable conditions was one reason for the high residual macromolecule content andhigh RMP. The outgoing protein content was relatively high in all digesters and improving the degradation of proteinsrepresents one important way to increase the VS reduction and methane production in biogas plants. Post-treatment
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3.
  • Sandås, Kristofer, et al. (författare)
  • Nanometa Live : a user-friendly application for real-time metagenomic data analysis and pathogen identification
  • 2024
  • Ingår i: Bioinformatics. - : Oxford University Press. - 1367-4803 .- 1367-4811. ; 40:3
  • Tidskriftsartikel (refereegranskat)abstract
    • Summary: Nanometa Live presents a user-friendly interface designed for real-time metagenomic data analysis and pathogen identification utilizing Oxford Nanopore Technologies’ MinION and Flongle flow cells. It offers an efficient workflow and graphical interface for the visualization and interpretation of metagenomic data as it is being generated. Key features include automated BLAST validation, streamlined handling of custom Kraken2 databases, and a simplified graphical user interface for enhanced user experience. Nanometa Live is particularly notable for its capability to run without constant internet or server access once installed, setting it apart from similar tools. It provides a comprehensive view of taxonomic composition and facilitates the detection of user-defined pathogens or other species of interest, catering to both researchers and clinicians.Availability and implementation: Nanometa Live has been implemented as a local web application using the Dash framework with Snakemake handling the data processing. The source code is freely accessible on the GitHub repository at https://github.com/FOIBioinformatics/nanometa_live and it is easily installable using Bioconda. It includes containerization support via Docker and Singularity, ensuring ease of use, reproducibility, and portability.
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4.
  • Cornelissen, Johannes H C, et al. (författare)
  • Global negative vegetation feedback to climate warming responses of leaf litter decomposition rates in cold biomes
  • 2007
  • Ingår i: Ecology Letters. - : Wiley. - 1461-023X .- 1461-0248. ; 10:7, s. 619-627
  • Tidskriftsartikel (refereegranskat)abstract
    • Whether climate change will turn cold biomes from large long-term carbon sinks into sources is hotly debated because of the great potential for ecosystem-mediated feedbacks to global climate. Critical are the direction, magnitude and generality of climate responses of plant litter decomposition. Here, we present the first quantitative analysis of the major climate-change-related drivers of litter decomposition rates in cold northern biomes worldwide.Leaf litters collected from the predominant species in 33 global change manipulation experiments in circum-arctic-alpine ecosystems were incubated simultaneously in two contrasting arctic life zones. We demonstrate that longer-term, large-scale changes to leaf litter decomposition will be driven primarily by both direct warming effects and concomitant shifts in plant growth form composition, with a much smaller role for changes in litter quality within species. Specifically, the ongoing warming-induced expansion of shrubs with recalcitrant leaf litter across cold biomes would constitute a negative feedback to global warming. Depending on the strength of other (previously reported) positive feedbacks of shrub expansion on soil carbon turnover, this may partly counteract direct warming enhancement of litter decomposition.
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5.
  • Gumbel, Jörg, et al. (författare)
  • The MATS satellite mission - gravity wave studies by Mesospheric Airglow/Aerosol Tomography and Spectroscopy
  • 2020
  • Ingår i: Atmospheric Chemistry And Physics. - : COPERNICUS GESELLSCHAFT MBH. - 1680-7316 .- 1680-7324. ; 20:1, s. 431-455
  • Tidskriftsartikel (refereegranskat)abstract
    • Global three-dimensional data are a key to understanding gravity waves in the mesosphere and lower thermosphere. MATS (Mesospheric Airglow/Aerosol Tomography and Spectroscopy) is a new Swedish satellite mission that addresses this need. It applies space-borne limb imaging in combination with tomographic and spectroscopic analysis to obtain gravity wave data on relevant spatial scales. Primary measurement targets are O-2 atmospheric band dayglow and nightglow in the near infrared, and sunlight scattered from noctilucent clouds in the ultraviolet. While tomography provides horizontally and vertically resolved data, spectroscopy allows analysis in terms of mesospheric temperature, composition, and cloud properties. Based on these dynamical tracers, MATS will produce a climatology on wave spectra during a 2-year mission. Major scientific objectives include a characterization of gravity waves and their interaction with larger-scale waves and mean flow in the mesosphere and lower thermosphere, as well as their relationship to dynamical conditions in the lower and upper atmosphere. MATS is currently being prepared to be ready for a launch in 2020. This paper provides an overview of scientific goals, measurement concepts, instruments, and analysis ideas.
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8.
  • Samuelsson, Linda, 1973, et al. (författare)
  • Using NMR metabolomics to identify responses of an environmental estrogen in blood plasma of fish
  • 2006
  • Ingår i: Aquatic Toxicology (Amsterdam, Netherlands). - : Elsevier BV. - 0166-445X. ; 78:4, s. 341-349
  • Tidskriftsartikel (refereegranskat)abstract
    • Nuclear magnetic resonance (NMR) based metabolomics in combination with multivariate data analysis may become valuable tools to study environmental effects of pharmaceuticals and other chemicals in aquatic organisms. To explore the usefulness of this approach in fish, we have used H-1 NMR metabolomics to compare blood plasma and plasma lipid extracts from rainbow trout exposed to the synthetic contraceptive estrogen ethinylestradiol (EE2) with plasma from control fish. The plasma metabolite profile was affected in fish exposed to 10 ng/L but not 0.87 ng/L of EE2, which was in agreement with an induced vitellogenin synthesis in the high dose group only, as measured by ELISA. The main affected metabolites were vitellogenin, alanine, phospholipids and cholesterol. The responses identified by this discovery-driven method could be put in context with previous knowledge of the effects of estrogens on fish. This adds confidence to the approach of using NMR metabolomics to identify environmental effects of pharmaceuticals and other contaminants. (c) 2006 Elsevier B.V. All rights reserved.
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9.
  • Sundberg, Jenny, 1978, et al. (författare)
  • Variation in ozone concentration in relation to local climate in south-west Sweden
  • 2006
  • Ingår i: Water Air and Soil Pollution. - : Springer Science and Business Media LLC. - 0049-6979 .- 1573-2932. ; 173:1-4, s. 339-354
  • Tidskriftsartikel (refereegranskat)abstract
    • Ozone (O-3) concentration and air temperature/relative humidity were monitored using diffusive samplers (weekly, 3 m above ground) and Tinytag loggers (10 min sampling, 1 m above ground, self-ventilating radiation shields), respectively, in the forested landscape of south-west Sweden, 40 km north-east of Gothenburg. Two forest sites were included, one at a hilltop (175 m a.s.l.), and one in a nearby (similar to 1 km) valley (110 m a.s.l.). In addition, a valley site (similar to 3 km from the forest sites, 60 m a.s.l.) in an agricultural landscape was included, where ozone was measured using both a UV-based monitor and diffusive sampling. At the agricultural site measurements of temperature and relative humidity were made using a radiation shield with forced ventilation and with Tinytags, as on the forest sites, in addition to observations of wind speed and the vertical temperature gradient. Furthermore, comparison with O-3 concentrations at urban and coastal sites in the region was made. The temperature dependent, systematic error of using a self-ventilating radiation shield was estimated and corrected for. It was found that the elevated forested site experienced higher O-3 concentrations and lower evening cooling rates in calm situations as compared to the forest site in the valley and in particular as compared to the agricultural site. This can be explained by a stronger coupling with the planetary boundary layer at the elevated site and more pronounced night-inversions at the valley sites. The difference in weekly O-3 concentration between the two forested sites was correlated with the difference in average minimum night-time temperature. The coastal site had the highest ozone concentrations, related largely, but not fully, to higher night-time O-3 concentrations. The urban site showed a depression in O-3 concentration associated with the combination of large NO emissions and slow air mixing during the morning traffic rush hours.
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10.
  • Bråkenhielm, Carl-Reinhold (bidragsgivare, creator_code:cre_t)
  • Kunskapsläget på kärnavfallsområdet 2022 : Samhället, tekniken och etiken
  • 2022
  • Rapport (populärvet., debatt m.m.)abstract
    • Kärnavfallsrådet (Statens råd för kärnavfallsfrågor) är en tvärvetenskaplig kommitté som har i uppdrag att ge regeringen råd i frågor om använt kärnbränsle, kärnavfall och rivning av kärntekniska anläggningar, (M 1992:A Kärnavfallsrådet. Dir. 2018:18). I februari vartannat år ger Kärnavfallsrådet sin självständiga bedömning av det aktuella läget inom kärnavfallsområdet. Bedömningen presenteras i form av en kunskapslägesrapport. Syftet med rapporten är att upp-märksamma och beskriva frågor som Kärnavfallsrådet anser viktiga och att redogöra för rådets synpunkter i dessa. Kärnavfallsrådet överlämnar härmed till regeringen årets kunskapslägesrapport SOU 2022:7 Kunskapsläget på kärnavfallsområdet 2022 – Samhället, tekniken och etiken.Bakom denna rapport står samtliga ledamöter och sakkunniga i Kärnavfallsrådet. Kärnavfallsrådets vetenskapliga sekreterare Johanna Swedin har varit projektledare för arbetet med kunskapslägesrapporten.Rapporterna om kunskapsläget på kärnavfallsområdet åren 1998, 2001, 2004, 2007, 2010, 2011, 2012, 2013, 2014, 2015, 2016, 2017, 2018 och 2020 finns även tillgängliga i en engelsk version. Rådet kommer att publicera en engelsk översättning av årets rapport under våren 2022.
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