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Sökning: WFRF:(Karlsson R) > Mälardalens universitet

  • Resultat 1-3 av 3
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1.
  • Chiu, D T, et al. (författare)
  • Chemical transformations in individual ultrasmall biomimetic containers
  • 1999
  • Ingår i: Science. - Stanford Univ, Dept Chem, Stanford, CA 94305 USA. Univ Gothenburg, Dept Chem, S-41296 Gothenburg, Sweden. Pomona Coll, Dept Chem, Claremont, CA 91711 USA. : AMER ASSOC ADVANCEMENT SCIENCE. - 0036-8075 .- 1095-9203. ; 283:5409, s. 1892-1895
  • Tidskriftsartikel (refereegranskat)abstract
    • Individual phospholipid vesicles, 1 to 5 micrometers in diameter, containing a single reagent or a complete reaction system, were immobilized with an infrared laser optical trap or by adhesion to modified borosilicate glass surfaces. Chemical transformations were initiated either by electroporation or by electrofusion, in each case through application of a short (10-microsecond), intense (20 to 50 kilovolts per centimeter) electric pulse delivered across ultramicroelectrodes. Product formation was monitored by far-field laser fluorescence microscopy. The ultrasmall characteristic of this reaction volume led to rapid diffusional mixing that permits the study of fast chemical kinetics. This technique is also well suited for the study of reaction dynamics of biological molecules within lipid-enclosed nanoenvironments that mimic cell membranes.
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2.
  • Chiu, D T, et al. (författare)
  • Manipulating the biochemical nanoenvironment around single molecules contained within vesicles
  • 1999
  • Ingår i: Chemical Physics. - Univ Gothenburg, Dept Chem, SE-41296 Gothenburg, Sweden. Stanford Univ, Dept Chem, Stanford, CA 94305 USA. : ELSEVIER. - 0301-0104 .- 1873-4421. ; 247:1, s. 133-139
  • Tidskriftsartikel (refereegranskat)abstract
    • A method to study single-molecule reactions confined in a biomimetic container is described. The technique combines rapid vesicle preparation, optical trapping and fluorescence confocal microscopy for performing simultaneous single-vesicle trapping and single-molecule detection experiments. The collisional environment between a single enzyme and substrate inside a vesicle is characterized by a Brownian dynamics Monte Carlo simulation. (C) 1999 Elsevier Science B.V. All rights reserved.
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3.
  • Cornelissen, Johannes H C, et al. (författare)
  • Global negative vegetation feedback to climate warming responses of leaf litter decomposition rates in cold biomes
  • 2007
  • Ingår i: Ecology Letters. - : Wiley. - 1461-023X .- 1461-0248. ; 10:7, s. 619-627
  • Tidskriftsartikel (refereegranskat)abstract
    • Whether climate change will turn cold biomes from large long-term carbon sinks into sources is hotly debated because of the great potential for ecosystem-mediated feedbacks to global climate. Critical are the direction, magnitude and generality of climate responses of plant litter decomposition. Here, we present the first quantitative analysis of the major climate-change-related drivers of litter decomposition rates in cold northern biomes worldwide.Leaf litters collected from the predominant species in 33 global change manipulation experiments in circum-arctic-alpine ecosystems were incubated simultaneously in two contrasting arctic life zones. We demonstrate that longer-term, large-scale changes to leaf litter decomposition will be driven primarily by both direct warming effects and concomitant shifts in plant growth form composition, with a much smaller role for changes in litter quality within species. Specifically, the ongoing warming-induced expansion of shrubs with recalcitrant leaf litter across cold biomes would constitute a negative feedback to global warming. Depending on the strength of other (previously reported) positive feedbacks of shrub expansion on soil carbon turnover, this may partly counteract direct warming enhancement of litter decomposition.
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  • Resultat 1-3 av 3
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Karlsson, A. (2)
Strömberg, Anette (2)
Zare, R. N. (2)
Chiu, D T (2)
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Mälardalens universitet  (3)Ta bort avgränsningen
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