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- Miliotis, Tasso, et al.
(författare)
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Protein identification platform utilizing micro dispensing technology interfaced to matrix-assisted laser desorption ionization time-of-flight mass spectrometry
- 2000
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Ingår i: Journal of Chromatography A. - 0021-9673. ; 886:1-2, s. 99-110
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Tidskriftsartikel (refereegranskat)abstract
- An integrated protein microcharacterization/identification platform has been developed. The system has been designed to allow a high flexibility in order to tackle challenging analytical problems. The platform comprises a cooled microautosampler, an integrated system for microcolumn HPLC, and a capillary reversed-phase column that is interfaced to matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) system via a low internal volume flow-through microdispenser. The chromatographic separation is continuously transferred onto a MALDI target plate as discrete spots as the dispenser ejects bursts of droplets of the column effluent in a precise array pattern. A refrigerated microfraction collector was coupled to the outlet of the flow-through microdispenser enabling enrichment and re-analysis of interesting fractions. The use of target plates pre-coated with matrix simplified and increased the robustness of the system. By including a separation step prior to the MALDI-TOF-MS analysis and hereby minimizing suppression effects allowed us to obtain higher sequence coverage of proteins compared to conventional MALDI sample preparation methodology. Additionally, synthetic peptides corresponding to autophosphorylated forms of the tryptic fragment 485-496 (ALGADDSYYTAR) of tyrosine kinase ZAP-70 were identified at sensitivities reaching 150 amol. Copyright (C) 2000 Elsevier Science B.V.
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- Miliotis, Tasso, et al.
(författare)
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Ready-made matrix-assisted laser desorption/ionization target plates coated with thin matrix layer for automated sample deposition in high-density array format.
- 2002
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Ingår i: Rapid Communications in Mass Spectrometry. - : Wiley. - 1097-0231 .- 0951-4198. ; 16:2, s. 117-126
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Tidskriftsartikel (refereegranskat)abstract
- The methodology for ready-made matrix-assisted laser desorption/ionization (MALDI) target plates covered with an optimized thin layer consisting of matrix and nitrocellulose has been developed. Piezoelectric microdispensing enabled sample depositions in a high-density array format of 2000 sample depositions on a conventionally sized target plate (45 x 47 mm). The sample depositions were made reproducibly in a fully automated mode by using an in-house developed computer-controlled piezoelectric flow-through microdispenser. Additionally, the piezoelectric technique facilitated significant analyte enrichment that increased the detection sensitivity. The MS signal was obtained rapidly, generally within ten laser pulses. An airbrush device was used to generate a fine spray of matrix and nitrocellulose dissolved in acetone. The acetone evaporated instantly when reaching the target plate leaving the entire surface with a thin and uniform matrix/nitrocellulose coating consisting of very small crystals of matrix embedded in the nitrocellulose. These crystals acted as a seed-layer on subsequent analyte depositions, rendering homogeneous sample spots when using alpha-cyano-4-hydroxycinnamic acid (CHCA) as matrix. The relative standard deviation of the signal intensity between spots was (20-30)% (n = 30). The detection sensitivity was improved by restricting the sample spot diameter to 300 microm. The spot size was affected by the deposition rate and the evaporation rate of the dispensed sample volume. Mass spectra of a 25-amol peptide mixture deposition were successfully recorded.
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- Strandberg, Gustav, 1977-
(författare)
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High-resolution simulations of two cold palaeo climates in Europe : MIS 3 and LGM
- 2015
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The study of past climate is important because it increases our understanding of how the climate system works. Past climate is often reconstructed by using proxies (that is observations of things that tell something about past climate, for example tree rings, pollen in lake sediments and fossils). Model simulations of past climate further increases the knowledge since it has the possibility to gap the space and time between the sparse and scattered proxy observations, since a model simulation gives relatively continuous information about the whole simulated area. Model simulations can also give internally coherent information about parameters that is not easily reconstructed from proxies (for example heat fluxes). In this thesis two periods in the past are simulated by climate models: the Marine Isotope Stage 3 (MIS 3), 44 000 years ago, and the Last Glacial Maximum (LGM), 21 000 years ago. Both periods are characterised by low temperature, low sea level and low level of carbon dioxide. The topography in northern Europe is dominated by ice sheets covering Iceland, Norway and parts of Sweden at MIS3; and more extensive ice sheets covering Iceland, Scandinavia, the British Isles and Northern Germany at LGM. These periods are firstly simulated by a global climate model. Those simulations are subsequently used in a regional climate model to increase the level of detail over Europe. To make the regional climate model simulation more realistic vegetation simulated by a dynamical vegetation model is used in the regional climate model. The climate models simulate European climates much colder than today, especially at LGM. The temperature differences ranges from 5 to 45 °C colder than today; the largest differences being at the ice sheets where the perennial ice cover and the high altitude keep temperatures low. Precipitation is reduced with as much as almost 100 % in northern Europe due to reduced evaporation. Precipitation is increased with as much as 100 % in parts of southern Europe due to changes in atmospheric circulation. The simulations are in broad agreement with proxies, although there are differences. The vegetation model simulates tundra like vegetation (herbs and shrubs) in the ice-free parts of central and southern Europe. The eastern parts of Europe are dominated by needle-leaved trees. The short and cool summers limit vegetation. The simulated vegetation is in broad agreement with reconstructions. Sensitivity studies of vegetation show that changed vegetation can change the monthly mean temperature with 1-3 °C in some seasons and regions. The response depends on regional surface characteristics. Sensitivity studies of ice sheets show that the simulated climate is consistent with the assumptions about the ice sheet extent made in the simulation. The simulated climate is cold enough in northern Europe to support the ice sheet, and warm enough in southern Europe to prevent the ice sheet from expanding in this direction. A removal of the ice sheet would only have an effect on the local scale in the vicinity of the ice sheet, but this experiment did not include changes in the large-scale global atmospheric circulation. Although the regional climate model simulations are to a large degree depending on the global climate model simulations they provide new information. When comparing proxies with model data or studying local/regional climatic features (such as the interplay between climate and vegetation) high horizontal resolution, as in the regional climate model, is important.
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- Strandberg, Gustav, 1977-
(författare)
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Modelling regional climate-vegetation interactions in Europe : A palaeo perspective
- 2017
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Studies in paleoclimate are important because they give us knowledge about how the climate system works and puts the current climate change in necessary perspective. By studying (pre)historic periods we increase our knowledge not just about these periods, but also about the processes that are important for climatic variations and changes. This thesis deals mainly with the interaction between climate and vegetation. Vegetation changes can affect climate in many different ways. These effects can be divided into two main categories: biogeochemical and biogeophysical processes. This thesis studies the biogeophysical effects of vegetation changes on climate in climate models. Climate models are a necessary tool for investigating how climate responds to changes in the climate system, as well as for making predictions of future climate. The biogeophysical processes are strongly related to characteristics of the land surface. Vegetation changes alter the land surface’s albedo (ability to reflect incoming solar radiation), roughness and evapotranspiration (the sum of evaporation and tran-spiration), which in turn affects the energy fluxes between the land surface and the atmosphere and thereby the climate. It is not, however, evident in what way; denser vegetation (e.g. forest instead of grassland) gives decreased albedo, which results in higher temperature, but also increased evapotranspiration, which contrastingly results in lower temperature. Vegetation changes are in this thesis studied in four different (pre)historic periods: two very cold periods with no human influence (c. 44,000 and 21,000 years ago), one warm period with minor human influence (c. 6,000 years ago) and a cold period with substantial human influence (c. 200 years ago). In addition to that the present climate is studied. The combination of these periods gives an estimate of the effect of both natural and anthropogenic vegetation on climate in different climatic contexts. The results show that vegetation changes can change temperature with 1–3 °C depending on season and region. The response is not the same everywhere, but depends on local properties of the land surface. During the winter half of the year, the albedo effect is usually most important as the difference in albedo between forest and open land is very large. During the summer half of the year the evapotranspiration effect is usually most important as differences in albedo between different vegetation types are smaller. A prerequisite for differences in evapotranspiration is that there is sufficient amount of water available. In dry regions, evapotranspiration does not change much with changes in vegetation, which means that the albedo effect will dominate also in summer. The conclusion of these studies is that vegetation changes can have a considerable effect on climate, comparable to the effect of increasing amounts of greenhouse gases in scenarios of future climate. Thus, it is important to have an appropriate description of the vegetation in studies of past, present and future climate. This means that vegetation has the potential to work as a feedback mechanism to natural climatic variations, but also that man can alter climate by altering the vegetation. It also means that mankind may have influenced climate before we started to use fossil fuel. Consequently, vegetation changes can be used as a means to mitigate climate change locally.
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