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Search: WFRF:(Klein M) > Södertörn University

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1.
  • Rich, Rebecca L., et al. (author)
  • A global benchmark study using affinity-based biosensors
  • 2009
  • In: Analytical Biochemistry. - : Elsevier BV. - 0003-2697 .- 1096-0309. ; 386:2, s. 194-216
  • Journal article (peer-reviewed)abstract
    • To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used.
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2.
  • Rao, P. M, et al. (author)
  • The Information and Communication Technologies and Enforcement of Intellectual Property Rights : a Relationship Perspective
  • 2008
  • In: The Journal of World Intellectual Property. - : Wiley. - 1422-2213 .- 1747-1796. ; 11:2, s. 105-119
  • Journal article (peer-reviewed)abstract
    • Intellectual capital represents an increasingly important area in the world economy. Information and communication technologies (ICTs) have played an essential role in the globalization of the economy. The protection of intellectual property rights (IPRs) is an essential factor in international economic affairs. This article discusses the legal issues concerning proprietary and open software licensing and suggests alternatives to legally enforcing IPRs. Global technology networking and outsourcing have involved the use of open source software (OSS), and have implications for the enforcement of IPRs. Propriety and OSS licensing are analyzed in this article. Companies may adopt alternative strategies to the strict enforcement of IPRs. A relationship view of IPRs is presented, a perspective that adopts relationship marketing as a means of gaining profits from IPRs in the ICT sector. By establishing a relationship to customers, software producers may gain customer loyalty and commitment from users of their software and, in the long run, retain a position as a chosen supplier of software.
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