| 1. |
- Abariute, Laura, et al.
(författare)
-
Uptake of nanowires by human lung adenocarcinoma cells
- 2019
-
Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 14:6
-
Tidskriftsartikel (refereegranskat)abstract
- Semiconductor nanowires are increasingly used in optoelectronic devices. However, their effects on human health have not been assessed fully. Here, we investigate the effects of gallium phosphide nanowires on human lung adenocarcinoma cells. Four different geometries of nanowires were suspended in the cell culture for 48 hours. We show that cells internalize the nanowires and that the nanowires have no effect on cell proliferation rate, motility, viability and intracellular ROS levels. By blocking specific internalization pathways, we demonstrate that the nanowire uptake is the result of a combination of processes, requiring dynamin and actin polymerization, which suggests an internalization through macropinocytosis and phagocytosis.
|
|
| 2. |
- Berthing, Trine, et al.
(författare)
-
Pulmonary toxicity and translocation of gallium phosphide nanowires to secondary organs following pulmonary exposure in mice
- 2023
-
Ingår i: Journal of Nanobiotechnology. - 1477-3155. ; 21:1
-
Tidskriftsartikel (refereegranskat)abstract
- Background: III-V semiconductor nanowires are envisioned as being integrated in optoelectronic devices in the near future. However, the perspective of mass production of these nanowires raises concern for human safety due to their asbestos- and carbon nanotube-like properties, including their high aspect ratio shape. Indeed, III-V nanowires have similar dimensions as Mitsui-7 multi-walled carbon nanotubes, which induce lung cancer by inhalation in rats. It is therefore urgent to investigate the toxicological effects following lung exposure to III-V nanowires prior to their use in industrial production, which entails risk of human exposure. Here, female C57BL/6J mice were exposed to 2, 6, and 18 µg (0.12, 0.35 and 1.1 mg/kg bw) of gallium phosphide (III-V) nanowires (99 nm diameter, 3.7 μm length) by intratracheal instillation and the toxicity was investigated 1, 3, 28 days and 3 months after exposure. Mitsui-7 multi-walled carbon nanotubes and carbon black Printex 90 nanoparticles were used as benchmark nanomaterials. Results: Gallium phosphide nanowires induced genotoxicity in bronchoalveolar lavage cells and acute inflammation with eosinophilia observable both in bronchoalveolar lavage and lung tissue (1 and 3 days post-exposure). The inflammatory response was comparable to the response following exposure to Mitsui-7 multi-walled carbon nanotubes at similar dose levels. The nanowires underwent partial dissolution in the lung resulting in thinner nanowires, with an estimated in vivo half-life of 3 months. Despite the partial dissolution, nanowires were detected in lung, liver, spleen, kidney, uterus and brain 3 months after exposure. Conclusion: Pulmonary exposure to gallium phosphide nanowires caused similar toxicological effects as the multi-walled carbon nanotube Mitsui-7. Graphical Abstract: [Figure not available: see fulltext.]
|
|
| 3. |
- Cedervall, Tommy, et al.
(författare)
-
Food chain transport of nanoparticles affects behaviour and fat metabolism in fish.
- 2012
-
Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 7:2
-
Tidskriftsartikel (refereegranskat)abstract
- Nano-sized (10(-9)-10(-7) m) particles offer many technical and biomedical advances over the bulk material. The use of nanoparticles in cosmetics, detergents, food and other commercial products is rapidly increasing despite little knowledge of their effect on organism metabolism. We show here that commercially manufactured polystyrene nanoparticles, transported through an aquatic food chain from algae, through zooplankton to fish, affect lipid metabolism and behaviour of the top consumer. At least three independent metabolic parameters differed between control and test fish: the weight loss, the triglycerides∶cholesterol ratio in blood serum, and the distribution of cholesterol between muscle and liver. Moreover, we demonstrate that nanoparticles bind to apolipoprotein A-I in fish serum in-vitro, thereby restraining them from properly utilising their fat reserves if absorbed through ingestion. In addition to the metabolic effects, we show that consumption of nanoparticle-containing zooplankton affects the feeding behaviour of the fish. The time it took the fish to consume 95% of the food presented to them was more than doubled for nanoparticle-exposed compared to control fish. Since many nano-sized products will, through the sewage system, end up in freshwater and marine habitats, our study provides a potential bioassay for testing new nano-sized material before manufacturing. In conclusion, our study shows that from knowledge of the molecular composition of the protein corona around nanoparticles it is possible to make a testable molecular hypothesis and bioassay of the potential biological risks of a defined nanoparticle at the organism and ecosystem level.
|
|
| 4. |
|
|
| 5. |
- Dabkowska, Aleksandra, et al.
(författare)
-
Surface nanostructures for fluorescence probing of supported lipid bilayers on reflective substrates.
- 2015
-
Ingår i: Nanoscale. - : Royal Society of Chemistry (RSC). - 2040-3372 .- 2040-3364. ; 7:43, s. 18020-18024
-
Tidskriftsartikel (refereegranskat)abstract
- The fluorescence interference contrast (FLIC) effect prevents the use of fluorescence techniques to probe the continuity and fluidity of supported lipid bilayers on reflective materials due to a lack of detectable fluorescence. Here we show that adding nanostructures onto reflective surfaces to locally confer a certain distance between the deposited fluorophores and the reflecting surface enables fluorescence detection on the nanostuctures. The nanostructures consist of either deposited nanoparticles or epitaxial nanowires directly grown on the substrate and are designed such that they can support a lipid bilayer. This simple method increases the fluorescence signal sufficiently to enable bilayer fluorescence detection and to observe the recovery of fluorescence after photobleaching in order to assess lipid bilayer formation on any reflective surface.
|
|
| 6. |
- Kumar, Saroj, et al.
(författare)
-
Antibodies Covalently Immobilized on Actin Filaments for Fast Myosin Driven Analyte Transport
- 2012
-
Ingår i: PLOS ONE. - : Public Library of Science. - 1932-6203. ; 7:10
-
Tidskriftsartikel (refereegranskat)abstract
- Biosensors would benefit from further miniaturization, increased detection rate and independence from external pumps and other bulky equipment. Whereas transportation systems built around molecular motors and cytoskeletal filaments hold significant promise in the latter regard, recent proof-of-principle devices based on the microtubule-kinesin motor system have not matched the speed of existing methods. An attractive solution to overcome this limitation would be the use of myosin driven propulsion of actin filaments which offers motility one order of magnitude faster than the kinesin-microtubule system. Here, we realized a necessary requirement for the use of the actomyosin system in biosensing devices, namely covalent attachment of antibodies to actin filaments using heterobifunctional cross-linkers. We also demonstrated consistent and rapid myosin II driven transport where velocity and the fraction of motile actin filaments was negligibly affected by the presence of antibody-antigen complexes at rather high density (>20 mu m(-1)). The results, however, also demonstrated that it was challenging to consistently achieve high density of functional antibodies along the actin filament, and optimization of the covalent coupling procedure to increase labeling density should be a major focus for future work. Despite the remaining challenges, the reported advances are important steps towards considerably faster nanoseparation than shown for previous molecular motor based devices, and enhanced miniaturization because of high bending flexibility of actin filaments.
|
|
| 7. |
- Lard, Mercy, et al.
(författare)
-
Biosensing using arrays of vertical semiconductor nanowires : mechanosensing and biomarker detection
- 2019
-
Ingår i: Nanotechnology. - : IOP Publishing. - 0957-4484 .- 1361-6528. ; 30:21
-
Tidskriftsartikel (refereegranskat)abstract
- Due to their high aspect ratio and increased surface-to-foot-print area, arrays of vertical semiconductor nanowires are used in numerous biological applications, such as cell transfection and biosensing. Here we focus on two specific valuable biosensing approaches that, so far, have received relatively limited attention in terms of their potential capabilities: cellular mechanosensing and lightguiding-induced enhanced fluorescence detection. Although proposed a decade ago, these two applications for using vertical nanowire arrays have only very recently achieved significant breakthroughs, both in terms of understanding their fundamental phenomena, and in the ease of their implementation. We review the status of the field in these areas and describe significant findings and potential future directions.
|
|
| 8. |
- Lard, Mercy, et al.
(författare)
-
Detection of Single Actin Filaments at Fluorescence Interference Contrast Checkpoints
- 2012
-
Ingår i: Biophysical Journal. - : Biophysical Society. - 0006-3495 .- 1542-0086. ; 102:3 Suppl. 1, s. 727A-727A
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- A number of emerging concepts for on-chip biotechnologies replace micro-fluidic flow by active, molecular-motor driven transport of filaments. Examples include applications in bio-simulation, diagnostics, and drug screening. Here we employ actomyosin molecular motors, embedded in nanostructures, as a platform for bio-simulation of the time evolution of motile objects in complex networks. A specific need for this type of application is detection of filaments at specific checkpoints in the device with high signal-to-noise ratio, for example to record the number and speed of filaments at a certain location in the device. To serve this need, we make use of fluorescence interference contrast (FLIC) at thin gold lines running perpendicular to nano-sized polymer resist channels that guide filament motion. We have demonstrated that it is possible to track single or multiple filaments passing over these gold lines, using either an enhanced or quenched fluorescence signal. We will discuss the fine-tuning of the device design, development of an algorithm for analyzing the optical readout signal from these detectors, and explo-ration of the error limits of detection. The results will help establish the viability of active, motor-driven on-chip applications which, among other advantages, offer substantial potential for miniaturization due to the absence of a need for pumps. The results also open for automatic read-out of velocity inhigh-throughput motility assays e.g. for drug discovery or fundamental bio-physical investigations. This work is supported by MONAD, an EU-FP7 collaborative effort.
|
|
| 9. |
- Lard, Mercy, et al.
(författare)
-
Fluorescence resonance energy transfer between phenanthrene and PAMAM dendrimers.
- 2010
-
Ingår i: Physical chemistry chemical physics : PCCP. - : Royal Society of Chemistry (RSC). - 1463-9084 .- 1463-9076. ; 12, s. 9285-9291
-
Tidskriftsartikel (refereegranskat)abstract
- We describe herein an adsorption-induced energy transfer between phenanthrene, a major environmental pollutant, and a fluorescently labeled dendrimer acting as a host molecule. We find experimentally that such energy transfer is the most efficient at a solvent pH of 8 and for a phenanthrene ratio dendrimer molar ratio of 1 ratio 2. Using molecular dynamics simulations we show that the strongest binding interactions occur between phenanthrene and the primary amines of the dendrimer. The simulations provide evidence that at low pH, phenanthrene-phenanthrene interactions are favorable and compete with phenanthrene-dendrimer binding. This study offers a new scheme for detecting dendrimer molecular assembly and a physical basis for exploiting dendrimer nanotechnologies for water purification and environmental remediation.
|
|
| 10. |
- Lard, Mercy, et al.
(författare)
-
Molecular Motor Transport through Hollow Nanowires
- 2014
-
Ingår i: Nano letters (Print). - : American Chemical Society (ACS). - 1530-6984 .- 1530-6992. ; 14:6, s. 3041-3046
-
Tidskriftsartikel (refereegranskat)abstract
- Biomolecular motors offer self-propelled, directed transport in designed microscale networks and can potentially replace pump-driven nanofluidics. However, in existing systems, transportation is limited to the two-dimensional plane. Here we demonstrate fully one-dimensional (1D) myosin-driven motion of fluorescent probes (actin filaments) through 80 nm wide, Al2O3 hollow nanowires of micrometer length. The motor-driven transport is orders of magnitude faster than would be possible by passive diffusion. The system represents a necessary element for advanced devices based on gliding assays, for example, in lab-on-a-chip systems with channel crossings and in pumpless nanosyringes. It may also serve as a scaffold for bottom-up assembly of muscle proteins into actin ordered contractile units, mimicking the muscle sarcomere.
|
|
