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| 2. |
- Schoch, Conrad L., et al.
(författare)
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Finding needles in haystacks: linking scientific names, reference specimens and molecular data for Fungi
- 2014
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Ingår i: Database: The Journal of Biological Databases and Curation. - : Oxford University Press (OUP). - 1758-0463. ; 2014:bau061, s. 1-21
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Tidskriftsartikel (refereegranskat)abstract
- DNA phylogenetic comparisons have shown that morphology-based species recognition often underestimates fungal diversity. Therefore, the need for accurate DNA sequence data, tied to both correct taxonomic names and clearly annotated specimen data, has never been greater. Furthermore, the growing number of molecular ecology and microbiome projects using high-throughput sequencing require fast and effective methods for en masse species assignments. In this article, we focus on selecting and re-annotating a set of marker reference sequences that represent each currently accepted order of Fungi. The particular focus is on sequences from the internal transcribed spacer region in the nuclear ribosomal cistron, derived from type specimens and/or ex-type cultures. Re-annotated and verified sequences were deposited in a curated public database at the National Center for Biotechnology Information (NCBI), namely the RefSeq Targeted Loci (RTL) database, and will be visible during routine sequence similarity searches with NR_prefixed accession numbers. A set of standards and protocols is proposed to improve the data quality of new sequences, and we suggest how type and other reference sequences can be used to improve identification of Fungi.
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| 3. |
- Borde, Annika, 1979, et al.
(författare)
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Increased water transport in PDMS silicone films by addition of excipients
- 2012
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Ingår i: Acta Biomaterialia. - : Elsevier BV. - 1742-7061 .- 1878-7568. ; 8:2, s. 579-588
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Tidskriftsartikel (refereegranskat)abstract
- The development of new adhesive wound care products intended for an application over a prolonged time requires good water transporting properties of the adhesive for the maintenance of a suitable environment around the wound. The ability of polydimethylsiloxane (PDMS)-based silicone films to transport water has led to its use in skin pressure-sensitive adhesives and it would be advantageous to find ways for controlling or increasing water transport across PDMS films in order to be able to develop improved skin adhesives. In this study we present a way to increase water transport in such films by the addition of hydrophilic excipients. Three hydrophilic additives, highly water-soluble sucrose and the two superabsorbent polymers (SAP) Carbopol® and Pemulen™, were investigated. The effect of the excipients was characterized by water transport studies, swelling tests, scanning electron microscopy imaging and confocal microscopy. The cross-linked polymers, primarily Pemulen™, were efficient water transport enhancers, whereas sucrose did not show any effect. The effect of the additives seemed to correlate with their water binding capacity. For SAPs the formation of a percolating structure by swollen polymer was also suggested, which enhances water penetration by the higher volume fraction of areas with a higher diffusion constant (swollen SAP), leading to a faster transport through the entire film. © 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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| 4. |
- Wilson, R., et al.
(författare)
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Reconstructing Holocene climate from tree rings : The potential for a long chronology from the Scottish Highlands
- 2012
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Ingår i: The Holocene. - : SAGE Publications. - 0959-6836 .- 1477-0911. ; 22:1, s. 3-11
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Tidskriftsartikel (refereegranskat)abstract
- Despite promising research in the 1980s showing the potential of Scots pine (Pinus sylvestris L.) for the reconstruction of past summer temperatures in the Scottish Highlands, little dendroclimatic work has been attempted in this region since. This reflects, in part, the limited number of sparsely distributed remnant natural/ semi-natural pine woodlands in the Scottish Highlands and the lack of old growth forest therein. On average, most of the pine trees dated in this region are around 225 years in age. Here, we present the first results of an ongoing interdisciplinary initiative to develop a long Scottish chronology through the acquisition of modern, historical and subfossil pine material from the native pinewoods, historic structures and lakes of the Scottish Highlands. Radiocarbon dating of 25 subfossil pine timbers recovered from lake sediments identified the presence of preserved material covering the last 8000 years with initial clusters focused on the last two millennia and early-mid Holocene. Although developing a well-replicated 8000 year pine chronology will take many years, this preliminary study indicates that a millennial length pine chronology from the northwest Cairngorm region is a feasible and realistic objective in the near future. The importance of such a record in this climatically important sector of northwest Europe cannot be underestimated.
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| 5. |
- Cerveny, D., et al.
(författare)
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Neuroactive drugs and other pharmaceuticals found in blood plasma of wild European fish
- 2021
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Ingår i: Environmental International. - : Elsevier BV. - 0160-4120 .- 1873-6750. ; 146
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Tidskriftsartikel (refereegranskat)abstract
- To gain a better understanding of which pharmaceuticals could pose a risk to fish, 94 pharmaceuticals representing 23 classes were analyzed in blood plasma from wild bream, chub, and roach captured at 18 sites in Germany, the Czech Republic and the UK, respectively. Based on read across from humans, we evaluated the risks of pharmacological effects occurring in the fish for each measured pharmaceutical. Twenty-three compounds were found in fish plasma, with the highest levels measured in chub from the Czech Republic. None of the German bream had detectable levels of pharmaceuticals, whereas roach from the Thames had mostly low concentrations. For two pharmaceuticals, four individual Czech fish had plasma concentrations higher than the concentrations reached in the blood of human patients taking the corresponding medication. For nine additional compounds, determined concentrations exceeded 10% of the corresponding human therapeutic plasma concentration in 12 fish. The majority of the pharmaceuticals where a clear risk for pharmacological effects was identified targets the central nervous system. These include e.g. flupentixol, haloperidol, and risperidone, all of which have the potential to affect fish behavior. In addition to identifying pharmaceuticals of environmental concern, the results emphasize the value of environmental monitoring of internal drug levels in aquatic wildlife, as well as the need for more research to establish concentration-response relationships. © 2020 The Author(s)
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