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- Blomberg, Anders, 1956, et al.
(författare)
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Establishing the barnacle Balanus improvisus as a potent invertebrate monitoring system in marine ecotoxicogenomics
- 2009
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Ingår i: SETAC Europe 19th Annual Meeting Abstract Book, Göteborg 31 May - 4 June, 2009.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- There is a need for potent invertebrate systems for assessing the impacts of environmental contaminants on marine ecosystems. Balanus improvisus, a marine athropod, has a number of promising characteristics that make it a good candidate in such efforts. We have conducted sequencing of a cDNA library from the cyprid larval stage and identified several detoxification systems as well as novel B. improvisus specific genes. To investigate the toxicological gene expression response in this organism, we performed a short-term exposure experiment of the cyprid larvae to two different biofouling substances. From a natural population of B. improvisus, 300 - 1000 cyprids were treated for 23 hours with 390nM CuO or with two different concentrations (10nM or 10μM) of meditomidine. Protein expression changes were studied by 2D-PAGE analysis after DIGE labelling. For gene expression analysis by DNA miroarrays total RNA was extracted and used for cDNA and cRNA/aRNA templates. Roughly 2000 B. improvisus genes were studied represented by 3000 different probes on the arrays (each in duplicates). Candidate genes were confirmed by qPCR. A number of protein expression changes were detected on the 2D gels as a result of the different treatments. Interestingly, the response to the different treatments clearly formed distinct groups in principle component analysis. The DNA microarray analysis revealed several genes as toxicity indicators, e.g. various heat shock proteins, some proteases and factors involved in regulatory processes (transcription factors). Our data indicate that B. improvisus may serve as a tool to evaluate the impacts of marine pollution, and thus to fill the niche as an important invertebrate marine model organism for ecotoxicology and environmental genomics.
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- Borgentun, Carl, 1979, et al.
(författare)
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Full characterization of a semiconductor laser beam by simultaneous capture of the near- and far-field
- 2010
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Ingår i: Conference Digest - IEEE International Semiconductor Laser Conference. - 0899-9406. - 9781424456833 ; , s. 127-128
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- We present a new measurement technique for fully characterizing a semiconductor laser beam. The reflections from both surfaces of a planoconvex lens are used to simultaneously capture the near- and far-field. The optical phase is then retrieved using the Gerchberg-Saxton algorithm with improved numerical operations.
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- Borgentun, Carl, 1979, et al.
(författare)
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Method for measuring reflectance of semiconductor disk laser gain element under optical pump excitation
- 2011
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Ingår i: Conference on Lasers and Electro-Optics (CLEO), Baltimore, USA, 1-6/5 2011. - Washington, D.C. : OSA.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- We present a new measurement method for measuring the spectral reflectance of a semiconductor disk laser gain element under optical pumping, providing valuable information on the spectral dependence of gain under close-to-normal operating conditions.
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- Borgentun, Carl, 1979, et al.
(författare)
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Optically pumped high-power semiconductor disk laser with gain element engineered for wide tunability
- 2010
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Ingår i: Proceedings of SPIE - The International Society for Optical Engineering. - : SPIE. - 0277-786X .- 1996-756X. - 9780819481931 ; 7720:1, s. 772014-8
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- The layer structure of the gain element in an optically pumped semiconductor disk laser (OP-SDL) was designed for wide tunability. This was achieved by a parametric optimization of the structure, which in effect balanced the spectrally varying influence of the gain of the quantum wells, the longitudinal distribution of the standing wave lasing field in the structure, and the degree of resonance in the subcavity formed between the distributed Bragg reflector at the bottom and the air-semiconductor interface at the top. The quality measure in the optimization was the spectral reflectance of the gain element for light incident from the external cavity at low power. This unsaturated reflectance was compared to its target function, which was constant at a specified value larger than unity over a wide, prescribed wavelength range. The fabricated gain element was used in a linear OP-SDL with a rotatable intra-cavity birefringent filter for wavelength tuning. The design principles for achieving wide tunability were experimentally validated by the strong agreement between measurements and simulations of the spectral threshold pump intensity. Furthermore, tuning experiments at high pump powers were performed showing that the lasing wavelength could be tuned from 967 nm to 1010 nm with a maximum output power of 2.6 W.
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