| 1. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 2. |
- Weinstein, John N., et al.
(författare)
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The cancer genome atlas pan-cancer analysis project
- 2013
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Ingår i: Nature Genetics. - : Springer Science and Business Media LLC. - 1061-4036 .- 1546-1718. ; 45:10, s. 1113-1120
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Forskningsöversikt (refereegranskat)abstract
- The Cancer Genome Atlas (TCGA) Research Network has profiled and analyzed large numbers of human tumors to discover molecular aberrations at the DNA, RNA, protein and epigenetic levels. The resulting rich data provide a major opportunity to develop an integrated picture of commonalities, differences and emergent themes across tumor lineages. The Pan-Cancer initiative compares the first 12 tumor types profiled by TCGA. Analysis of the molecular aberrations and their functional roles across tumor types will teach us how to extend therapies effective in one cancer type to others with a similar genomic profile. © 2013 Nature America, Inc. All rights reserved.
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| 3. |
- Ming, Yue, et al.
(författare)
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金属镁的氧化及氧化机理研究进展
- 2021
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Ingår i: Cailiao Daobao/Materials Reports. - 1005-023X. ; 35:19, s. 19134-19141
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Forskningsöversikt (refereegranskat)abstract
- As the lightest commercial metal structure material, magnesium alloy shows a wide application prospect in aerospace, automobile, 3C pro-ducts and other fields. At the same time, it is of great strategic significance to promote the application of magnesium alloy materials, in the face of the increasing shortage of iron and aluminum resources in the world and the dilemma of a large number of imported iron and aluminum ores. Compared with common steel and aluminum alloy, the research and development of magnesium alloy are not enough, and its application is also limited. The poor corrosion resistance of magnesium alloy is partly due to the high chemical activity of magnesium and the lack of protective effect of the film formed on the surface. Especially at high temperature, magnesium and its alloys are easy to oxidize, even burn, and release a lot of heat, which has become one of the bottlenecks limiting the extensive application of magnesium alloys. In recent years, a lot of researches have been carried out on the oxidation mechanism and influencing factors of magnesium and its alloys. It is considered that the oxidation of magnesium alloy is affected by factors, such as P-B value of oxide film, evaporation and diffusion of magnesium and so on. At present, the oxidation resistance of magnesium is improved by alloying. This provides theoretical support for the preparation of magnesium alloy with high oxidation resistance. At the same time, it expands the application prospect of magnesium alloy at high temperature, and will bring huge economic benefits to magnesium alloy industry. This paper summarizes the research progress of oxidation characteristics and mechanism of magnesium and its alloys at home and abroad. Firstly, the oxidation of magnesium is briefly introduced. Secondly, the mechanism and influencing factors of magnesium oxidation are analyzed, and the influence rules and mechanism of P-B value, diffusion, evaporation, microstructure and alloy elements on the oxidation behavior of magnesium are emphatically discussed. Finally, the shortcomings of the current research are summarized, and suggestions on the research direction of magnesium oxidation resistance are put forward.
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| 4. |
- Wu, Xiaofang, et al.
(författare)
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The effects of chondroitin and/or glucosamine on patients with Kashin-Beck disease
- 2016
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Ingår i: Science Insights Medicine. - : Insight Publisher. - 2378-8097. ; 2016
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Forskningsöversikt (refereegranskat)abstract
- Kashin-Beck disease (KBD), an endemic disease, is a special type of osteoarthritis (OA). Nowadays, due to prevention and treatment methods including selenium supplements, changing grains and water source as well as health education, the morbidity of KBD is reduced significantly as compared to that in the 1950s. However, many elderly adult KBD patients are still suffering from the degenerative changes of cartilage, pain, stiffness and deformation of joints, which are quite similar or even more serious than OA. Chondroitin sulfate and glucosamine have been widely used as symptomatic slow-acting drugs for the treatment of OA. Although their therapeutic effects, biochemical data, pharmacokinetics, preclinical studies, safety and economic evaluation have been well investigated in OA, they are not clearly studied in KBD. In this review, we will evaluate the clinical evidence (randomized controlled trials and non-randomized controlled trials), safeties and cost-effectiveness of chondroitin sulfate and glucosamine for the treatment of KBD. Moreover, the therapeutic mechanisms of chondroitin sulfate and glucosamine are also discussed in details.
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