| 1. |
- Chen, Li-li, et al.
(författare)
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Multilayered polyelectrolyte-coated gold nanorods as multifunctional optical contrast agents for cancer cell imaging
- 2010
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Ingår i: Journal of Zhejiang University: Science B. - 1673-1581. ; 11:6, s. 417-422
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Tidskriftsartikel (refereegranskat)abstract
- We report the application of multilayered polyelectrolyte-coated gold nanorods (GNRs) as multifunctional optical contrast agents for cancer cell imaging. The surface modification of GNRs improves their chemical stability and facilitates them to be taken up by cancer cells through electrostatic interaction. The unique longitudinal surface plasmon resonance property of GNRs makes them suitable as both "scattering contrast agents" and "Raman contrast agents". In our experiments, the staining of GNRs in cells was further confirmed by dark field microscopy and Raman microscopy. Our experiment results indicated that GNRs have great potential as multifunctional "optical contrast agents" for future in vivo animal imaging.
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| 2. |
- Jiang, Tao, et al.
(författare)
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Tetraphenylethene end-capped diketopyrrolopyrrole fluorogens with AIE and large two-photon absorption cross-sections features and application in bioimaging
- 2016
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Ingår i: Dyes and pigments. - : Elsevier. - 0143-7208 .- 1873-3743. ; 133, s. 201-213
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Tidskriftsartikel (refereegranskat)abstract
- In this work, a series of new diketopyrrolopyrrole-based dyes have been synthesized by connecting tetraphenylethene to the diketopyrrolopyrrole core. All the four compounds exhibit good aggregation induced emission property with nonemissive in the solution but strong red fluorescence in the aggregate or solid state. Also, these new dyes exhibit large two-photon absorption cross sections (a), in which the a data measured by the open aperture Z-scan technique are determined to be 150, 300, 1140 and 1016 GM for four dyes, respectively. In addition, compound with stilbene and four tetraphenylethene units (DPP-TPE-3) was used as the luminogen and encapsulated into nanoparticles for cell imaging and two-photon excited fluorescence blood vessels imaging. The result indicates that it can be used as the effective fluorescence probe for bioimaging and has great potential for bioapplications. (C) 2016 Elsevier Ltd. All rights reserved.
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| 3. |
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| 4. |
- Li, Xin, et al.
(författare)
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Study on enhancement of fluorescence with gold nanorods
- 2008
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Ingår i: Asia Optical Fiber Communication and Optoelectronic Exposition and Conference 2008. - : Optical Society of America. - 9781557528636
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Konferensbidrag (refereegranskat)abstract
- Enhancement of fluorescence of streptavidin conjugated with gold nanorods (GNRs) was observed. The impact of GNRs with different surface plasmon resonance bands and different concentrations to the efficiency of the fluorescence enhancement was studied.
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| 5. |
- Cai, Fuhong, et al.
(författare)
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Multifunctional optical imaging using dye-coated gold nanorods in a turbid medium
- 2011
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Ingår i: Journal of Biomedical Optics. - : SPIE-Intl Soc Optical Eng. - 1083-3668 .- 1560-2281. ; 16:1, s. 016002-
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Tidskriftsartikel (refereegranskat)abstract
- We report multifunctional optical imaging using dye-coated gold nanorods. Three types of useful information, namely, Raman, fluorescence signals, and absorption contrast, can be obtained from a phantom experiment. These three kinds of information are detected in a nanoparticle-doped-phantom using diffuse optical imaging. Our novel nanoparticle could be used as a multimodality marker for future bioimaging applications.
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| 6. |
- Chu, Liliang, et al.
(författare)
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Biocompatible near-infrared fluorescent nanoparticles for macro and microscopic in vivo functional bioimaging
- 2014
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Ingår i: Biomedical Optics Express. - 2156-7085. ; 5:11, s. 4076-4088
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Tidskriftsartikel (refereegranskat)abstract
- Near-infrared (NIR) imaging technology has been widely used for biomedical research and applications, since it can achieve deep penetration in biological tissues due to less absorption and scattering of NIR light. In our research, polymer nanoparticles with NIR fluorophores doped were synthesized. The morphology, absorption/emission features and chemical stability of the fluorescent nanoparticles were characterized, separately. NIR fluorescent nanoparticles were then utilized as bright optical probes for macro in vivo imaging of mice, including sentinel lymph node (SLN) mapping, as well as distribution and excretion monitoring of nanoparticles in animal body. Furthermore, we applied the NIR fluorescent nanoparticles in in vivo microscopic bioimaging via a confocal microscope. Under the 635 nm-CW excitation, the blood vessel architecture in the ear and the brain of mice, which were administered with nanoparticles, was visualized very clearly. The imaging depth of our one-photon microscopy, which was assisted with NIR fluorescent nanoprobes, can reach as deep as 500 mu m. Our experiments show that NIR fluorescent nanoparticles have great potentials in various deep-tissue imaging applications.
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| 7. |
- Jiang, Li, et al.
(författare)
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Raman reporter-coated gold nanorods and their applications in multimodal optical imaging of cancer cells
- 2011
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Ingår i: Analytical and Bioanalytical Chemistry. - : Springer Science and Business Media LLC. - 1618-2642 .- 1618-2650. ; 400:9, s. 2793-2800
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Tidskriftsartikel (refereegranskat)abstract
- We report the preparation of a kind of surface-enhanced Raman scattering (SERS) tags and explore their applications in multifunctional optical imaging of cancer cells. The proposed nanoparticles (SERS tags) are prepared by connecting dye molecules directly onto the surfaces of gold nanorods through Au-S or Au-N interactions. The dye molecules are used as Raman reporters, while gold nanorods are used as enhanced materials due to their localized surface plasmon resonance effect. Multilayered polymers are further coated onto the surfaces of the nanoparticles to reach better stability and biocompatibility. Gold nanorods with different aspect ratios and different dye molecules conjugated are compared in order to achieve the diversity of SERS tags and find out the optimized condition of SERS tags with the highest signal intensity. Our experiments show that the resulting nanoparticles, which are uptaken by cancer cells, can provide not only dark field cells images but also multiplexing SERS images.
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| 8. |
- Li, Xin, et al.
(författare)
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Nanoparticle-assisted DNA nanosensor
- 2008
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Ingår i: AOE 2007. - SHENZHEN : AOE. - 9780978921736 ; , s. 84-86
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Konferensbidrag (refereegranskat)abstract
- We report a sensitive nanosensor based on a micro-fluidic chip and nanoparticles to detect low concentrations of DNA. The emission of CdSe/ZnS-QDs linked with single strand DNAs was quenched by gold nanoparticles linked with the complementary sequences after hybridization. Sensitively detected signal of DNA was obtained from a 100 mu m capillary.
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| 9. |
- Qian, Jun, et al.
(författare)
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Fluorescence-surface enhanced Raman scattering co-functionalized gold nanorods as near-infrared probes for purely optical in vivo imaging
- 2011
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Ingår i: Biomaterials. - : Elsevier BV. - 0142-9612 .- 1878-5905. ; 32:6, s. 1601-1610
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Tidskriftsartikel (refereegranskat)abstract
- Gold nanorods (GNRs) have been widely used for bio-imaging. However, GNRs assisted optical in vivo deep tissue imaging is severely restricted due to signal attenuation, low contrast, complex process or low real-timing. To overcome these problems, we functionalized GNRs with both near-infrared (NIR) fluorescence and surface enhanced Raman scattering (SERS) and utilized these co-functionalized GNRs for purely optical in vivo imaging of live mice. Our proposed technology has the combined advantages of high real-timing, high imaging contrast and deep detection ability. The distribution and excretion of intravenously injected GNRs in deep tissues of live mice were observed in vivo for the first time through purely optical imaging. We also demonstrated successfully in vivo biomedical applications of the co-functionalized GNRs to sentinel lymph node (SLN) mapping and tumor targeting of mice. The present technology has great future potentials for disease diagnosis and clinical therapies.
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| 10. |
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