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Sökning: WFRF:(Li Li) > Högskolan Kristianstad

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1.
  • Kraus, Robert H. S., et al. (författare)
  • Genome wide SNP discovery, analysis and evaluation in mallard (Anas platyrhynchos)
  • 2011
  • Ingår i: BMC Genomics. - 1471-2164 .- 1471-2164. ; 12, s. 150-
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: Next generation sequencing technologies allow to obtain at low cost the genomic sequence information that currently lacks for most economically and ecologically important organisms. For the mallard duck genomic data is limited. The mallard is, besides a species of large agricultural and societal importance, also the focal species when it comes to long distance dispersal of Avian Influenza. For large scale identification of SNPs we performed Illumina sequencing of wild mallard DNA and compared our data with ongoing genome and EST sequencing of domesticated conspecifics. This is the first study of its kind for waterfowl. Results: More than one billion base pairs of sequence information were generated resulting in a 16x coverage of a reduced representation library of the mallard genome. Sequence reads were aligned to a draft domesticated duck reference genome and allowed for the detection of over 122,000 SNPs within our mallard sequence dataset. In addition, almost 62,000 nucleotide positions on the domesticated duck reference showed a different nucleotide compared to wild mallard. Approximately 20,000 SNPs identified within our data were shared with SNPs identified in the sequenced domestic duck or in EST sequencing projects. The shared SNPs were considered to be highly reliable and were used to benchmark non-shared SNPs for quality. Genotyping of a representative sample of 364 SNPs resulted in a SNP conversion rate of 99.7%. The correlation of the minor allele count and observed minor allele frequency in the SNP discovery pool was 0.72. Conclusion: We identified almost 150,000 SNPs in wild mallards that will likely yield good results in genotyping. Of these, similar to 101,000 SNPs were detected within our wild mallard sequences and similar to 49,000 were detected between wild and domesticated duck data. In the similar to 101,000 SNPs we found a subset of similar to 20,000 SNPs shared between wild mallards and the sequenced domesticated duck suggesting a low genetic divergence. Comparison of quality metrics between the total SNP set (122,000 + 62,000 = 184,000 SNPs) and the validated subset shows similar characteristics for both sets. This indicates that we have detected a large amount (similar to 150,000) of accurately inferred mallard SNPs, which will benefit bird evolutionary studies, ecological studies (e. g. disentangling migratory connectivity) and industrial breeding programs.
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2.
  • Kraus, Robert H. S., et al. (författare)
  • Genome wide SNP discovery, analysis and evaluation in mallard (Anas platyrhynchos)
  • 2011
  • Ingår i: BMC Genomics. - : BioMed Central Ltd.. - 1471-2164. ; 12
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: Next generation sequencing technologies allow to obtain at low cost the genomic sequence information that currently lacks for most economically and ecologically important organisms. For the mallard duck genomic data is limited. The mallard is, besides a species of large agricultural and societal importance, also the focal species when it comes to long distance dispersal of Avian Influenza. For large scale identification of SNPs we performed Illumina sequencing of wild mallard DNA and compared our data with ongoing genome and EST sequencing of domesticated conspecifics. This is the first study of its kind for waterfowl. Results: More than one billion base pairs of sequence information were generated resulting in a 16x coverage of a reduced representation library of the mallard genome. Sequence reads were aligned to a draft domesticated duck reference genome and allowed for the detection of over 122,000 SNPs within our mallard sequence dataset. In addition, almost 62,000 nucleotide positions on the domesticated duck reference showed a different nucleotide compared to wild mallard. Approximately 20,000 SNPs identified within our data were shared with SNPs identified in the sequenced domestic duck or in EST sequencing projects. The shared SNPs were considered to be highly reliable and were used to benchmark non-shared SNPs for quality. Genotyping of a representative sample of 364 SNPs resulted in a SNP conversion rate of 99.7%. The correlation of the minor allele count and observed minor allele frequency in the SNP discovery pool was 0.72. Conclusion: We identified almost 150,000 SNPs in wild mallards that will likely yield good results in genotyping. Of these, similar to 101,000 SNPs were detected within our wild mallard sequences and similar to 49,000 were detected between wild and domesticated duck data. In the similar to 101,000 SNPs we found a subset of similar to 20,000 SNPs shared between wild mallards and the sequenced domesticated duck suggesting a low genetic divergence. Comparison of quality metrics between the total SNP set (122,000 + 62,000 = 184,000 SNPs) and the validated subset shows similar characteristics for both sets. This indicates that we have detected a large amount (similar to 150,000) of accurately inferred mallard SNPs, which will benefit bird evolutionary studies, ecological studies (e. g. disentangling migratory connectivity) and industrial breeding programs.
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3.
  • Lakhan, Abdullah, et al. (författare)
  • Dynamic application partitioning and task-scheduling secure schemes for biosensor healthcare workload in mobile edge cloud
  • 2021
  • Ingår i: Electronics. - 2079-9292. ; 10:22, s. 1-30
  • Tidskriftsartikel (refereegranskat)abstract
    • Currently, the use of biosensor-enabled mobile healthcare workflow applications in mobile edge-cloud-enabled systems is increasing progressively. These applications are heavyweight and divided between a thin client mobile device and a thick server edge cloud for execution. Application partitioning is a mechanism in which applications are divided based on resource and energy parameters. However, existing application-partitioning schemes widely ignore security aspects for healthcare applications. This study devises a dynamic application-partitioning workload task-scheduling-secure (DAPWTS) algorithm framework that consists of different schemes, such as min-cut algorithm, searching node, energy-enabled scheduling, failure scheduling, and security schemes. The goal is to minimize the energy consumption of nodes and divide the application between local nodes and edge nodes by applying the secure min-cut algorithm. Furthermore, the study devises the secure-min-cut algorithm, which aims to migrate data between nodes in a secure form during application partitioning in the system. After partitioning the applications, the node-search algorithm searches optimally to run applications under their deadlines. The energy and failure schemes maintain the energy consumption of the nodes and the failure of the system. Simulation results show that DAPWTS outperforms existing baseline approaches by 30% in terms of energy consumption, deadline, and failure of applications in the system.
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4.
  • Lakhan, Abdullah, et al. (författare)
  • Dynamic application partitioning and task-scheduling secure schemes for biosensor healthcare workload in mobile edge cloud
  • 2021
  • Ingår i: Electronics. - : MDPI AG. - 2079-9292. ; 10:22, s. 1-30
  • Tidskriftsartikel (refereegranskat)abstract
    • Currently, the use of biosensor-enabled mobile healthcare workflow applications in mobile edge-cloud-enabled systems is increasing progressively. These applications are heavyweight and divided between a thin client mobile device and a thick server edge cloud for execution. Application partitioning is a mechanism in which applications are divided based on resource and energy parameters. However, existing application-partitioning schemes widely ignore security aspects for healthcare applications. This study devises a dynamic application-partitioning workload task-scheduling-secure (DAPWTS) algorithm framework that consists of different schemes, such as min-cut algorithm, searching node, energy-enabled scheduling, failure scheduling, and security schemes. The goal is to minimize the energy consumption of nodes and divide the application between local nodes and edge nodes by applying the secure min-cut algorithm. Furthermore, the study devises the secure-min-cut algorithm, which aims to migrate data between nodesin a secure form during application partitioning in the system. After partitioning the applications, the node-search algorithm searches optimally to run applications under their deadlines. The energy and failure schemes maintain the energy consumption of the nodes and the failure of the system. Simulation results show that DAPWTS outperforms existing baseline approaches by 30% in terms of energy consumption, deadline, and failure of applications in the system.
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5.
  • Li, Hezun, et al. (författare)
  • Corporate governance and innovation
  • 2019
  • Ingår i: A research agenda for entrepreneurship and innovation. - Cheltenham : Edward Elgar Publishing. - 978 1 78811 600 8
  • Bokkapitel (populärvet., debatt m.m.)
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7.
  • Li, Jie, et al. (författare)
  • A five year perspective of traffic pattern evolution in a residential broadband access network
  • 2012
  • Ingår i: Future Network & MobileSummit 2012. - 9781905824304
  • Konferensbidrag (refereegranskat)abstract
    • In this paper we describe a systematic study on long-term evolution of residential broadband Internet traffic covering 5 calendar years from June 2007 to May 2011. The traffic evolution is characterized both in the term of the total traffic volume, as well as the traffic volumes and shares for different application categories (file sharing, video streaming etc.), with the focus on comparing the traffic on the per IP user basis and among different broadband subscription groups. The results show that the average daily total traffic generated by each private end user increased only by about 33 % during the past 5 years. Further, the results show that the P2P filesharing has been dominating the network total traffic, but the daily file-sharing traffic volume per end user largely remains the same. Also, the daily streaming-media traffic volume per end user has increased dramatically by over 500% during the studied period of time. In the meantime, the daily web-browsing traffic volume per end user has increased by about 300%. Finally, a further investigation among 4 different FTTH broadband subscription groups with 1, 10 , 30, and 100 Mbit/s symmetric access speeds shows that the lower the access speed, the more diversified the end user traffic tend to be.
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8.
  • Li, Jie, et al. (författare)
  • Performance analysis of local caching replacement policies for internet video streaming services
  • 2014
  • Ingår i: SoftCOM 2014. - : IEEE Communications Society. - 9789532900521
  • Konferensbidrag (refereegranskat)abstract
    • In this work, the performance of 5 representative caching replacement policies was investigated and compared for caching Internet video-on-demand (VoD) in local access networks. Two measured traces of end-user requests were used in the analyses for two typical VoD services: TV-on-demand and user generated content represented by YouTube. The studied policies range from simple least recently used (LRU) and least frequently used (LFU) algorithms to more advanced ones denoted as LFU-dynamic lifespan (LFU-DL), Adaptive replacement cache (ARC) and Greedy-dual size frequency (GDSF). Our results show that the ARC policy always outperforms the other policies due to its adaptive nature and its ability to track changes in the traffic patterns. On the other hand, the simple LRU policy can also achieve a caching performance which is comparable to that of the more advanced ARC policy especially for the TV-on-demand service when the potential caching gain is high. On the contrary, the simple LFU policy always shows the poorest performance.However, by applying a proper lifespan supplement under the LFU-DL policy, the caching performance can be effectively enhanced to the level achievable using ARC and LRU policies. Moreover, the GDSF policy does not outperform simple LRU or LFU-DL, especially for YouTube video clips when the potential caching gain is relatively low. The advantage of GDSF manifested in our analysis is, however, its outstanding cache space usage efficiency among the five studied caching algorithms.
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9.
  • Li, Jie, et al. (författare)
  • YouTube traffic content analysis in the perspective of clip category and duration
  • 2013
  • Ingår i: 2013 Fourth International Conference on the Network of the Future (NOF 2013). - : IEEE Communications Society. - 9781479916429
  • Konferensbidrag (refereegranskat)abstract
    • In this work, we study YouTube traffic characteristics in a medium-sized Swedish residential municipal network that has ~ 2600 mainly FTTH broadband-connected households. YouTube traffic analyses were carried out in the perspective of video clip category and duration, in order to understand their impact on the potential local network caching gains. To the best of our knowledge, this is the first time systematic analysis of YouTube traffic content in the perspective of video clip category and duration in a residential broadband network. Our results show that the requested YouTube videoclips from the end users in the studied network were imbalanced in regarding the video categories and durations. The dominating video category was Music, both in terms of the total traffic share as well as the contribution to the overall potential local network caching gain. In addition, most of the requested video clips were between 2-5 min in duration, despite video clips with durations over 15 min were also popular among certain video categories, e.g. film videos.
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10.
  • Rolandsson Enes, Sara, et al. (författare)
  • Primary mesenchymal stem cells in human transplanted lungs are CD90/CD105 perivascularly located tissue-resident cells
  • 2014
  • Ingår i: BMJ Open Respiratory Research. - : BMJ Publishing Group. - 2052-4439. ; 1:1
  • Tidskriftsartikel (refereegranskat)abstract
    • BACKGROUND: Mesenchymal stem cells (MSC) have not only been implicated in the development of lung diseases, but they have also been proposed as a future cell-based therapy for lung diseases. However, the cellular identity of the primary MSC in human lung tissues has not yet been reported. This study therefore aimed to identify and characterise the ‘bona fide’ MSC in human lungs and to investigate if the MSC numbers correlate with the development of bronchiolitis obliterans syndrome in lung-transplanted patients. METHODS: Primary lung MSC were directly isolated or culture-derived from central and peripheral transbronchial biopsies of lung-transplanted patients and evaluated using a comprehensive panel of in vitro and in vivo assays. RESULTS: Primary MSC were enriched in the CD90/CD105 mononuclear cell fraction with mesenchymal progenitor frequencies of up to four colony-forming units, fibroblast/100 cells. In situ staining of lung tissues revealed that CD90/CD105 MSCs were located perivascularly. MSC were tissue-resident and exclusively donor lung-derived even in biopsies obtained from patients as long as 16 years after transplantation. Culture-derived mesenchymal stromal cells showed typical in vitro MSC properties; however, xenotransplantation into non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice showed that lung MSC readily differentiated into adipocytes and stromal tissues, but lacked significant in vivo bone formation. CONCLUSIONS: These data clearly demonstrate that primary MSC in human lung tissues are not only tissue resident but also tissue-specific. The identification and phenotypic characterisation of primary lung MSC is an important first step in identifying the role of MSC in normal lung physiology and pulmonary diseases.
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