| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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| 3. |
- Dong, Qibing, et al.
(författare)
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Regulating concentration of surface oxygen vacancies in Bi 2 MoO 6 /Bi-MOF for boosting photocatalytic ammonia synthesis
- 2024
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Ingår i: Journal of Catalysis. - 0021-9517 .- 1090-2694. ; 433
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Tidskriftsartikel (refereegranskat)abstract
- Surface oxygen vacancies (OVs) engineering has been widely adopted as an effective strategy to enhance photocatalytic performance. At present, photocatalytic systems capable of precisely regulating surface OVs concentrations, which could help illuminate the effects of the surface OVs concentration on N2 fixation activity, are still scarce. Herein, bismuth-based metal organic framework (Bi-MOF) was loaded onto the surface of Bi2MoO6 (BMO) as an operable platform, and the OVs concentration in the Bi-MOF component of BMO/Bi-MOF could be regulated by reduction of bismuth ions therein. Experimental results confirm that optimum construction of OVs in the Bi-MOF promotes the photoelectrons transfer from BMO to Bi-MOF, facilitating the activation of N2 at OVs. Consequently, the optimized catalyst shows superior performance in NH3 production, which reaches 125.78 μmol h−1 g−1, 21.4 higher than that of BMO. This work underline the significance of regulating surface OVs concentration, providing inspiration for the development of efficient OVs-modified photocatalysts.
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| 4. |
- Liang, Hanwei, et al.
(författare)
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Supporting zero-waste building: A novel spatial explicit material flow analysis model for construction waste
- 2024
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Ingår i: Journal of Cleaner Production. - 0959-6526. ; 456
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Tidskriftsartikel (refereegranskat)abstract
- The accurate quantification of construction waste (CW) is prerequisite for achieving zero-waste in the building sector. This study employs a Spatial Material Flow Analysis model considering building life cycle to estimate the spatio-temporal dynamics of CW in Nanjing, China, over two decades. Additionally, it employs an ARIMA model for forecasting CW generation over the forthcoming decade. During 2000 to 2020, Nanjing's CW witnessed a CW increase from 0.8 to 59.0 Tg, predominantly comprising non-metallic materials. Meanwhile, the spatial distribution of CW has widened, accompanied by the emergence of numerous CW hotspots, in parallel with economic and population growth. Future projections suggest a continuous yet decelerating increase in CW generation, highlighting a significant opportunity for resource recovery. The application of this hybrid approach not only enhances CW management accuracy but also supports the broader goal of fostering sustainable building practices. This research expects to enlighten the stakeholders committed to advancing sustainable construction and waste management strategies in the context of zero-waste buildings.
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| 5. |
- Liang, Hanwei, et al.
(författare)
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Towards net zero carbon buildings: Accounting the building embodied carbon and life cycle-based policy design for Greater Bay Area, China
- 2024
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Ingår i: Geoscience Frontiers. - 1674-9871. ; 15:3
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Tidskriftsartikel (refereegranskat)abstract
- Carbon mitigation of buildings is critical to promote a net-zero society. The international society has vigorously promoted “Net Zero Carbon Buildings” across the globe, and accounting for building carbon emissions is critical to support this initiative. Embodied carbon, which represents carbon emissions from the entire lifecycle of the buildings, is fundamental for realizing the idea of zero carbon. However, only limited studies have been conducted so far that take into account the city scale. This paper aimed to act as a first try to account for the embodied carbon emissions in buildings in 2020 for the Guangdong-Hong Kong Macau Greater Bay Area in China (GBA). We integrated remote sensing techniques such as night-time light data (NLT) and building material flows analysis to calculate and spatialize the newly generated building material stocks (MS). Based on the MS data, we further applied life cycle assessment (LCA) to assess the embodied carbon in the buildings. The results highlighted that over 163 million tons of embodied carbon in buildings of GBA are expected to be generated, from 497 million tons of newly generated building MS in 2020. The embodied carbon in each life cycle stage is valuable for further lifecycle-based policy designs for: (i) supporting the updating of the green building certification system with consideration of the embodied carbon; (ii) promoting the green building material application and certification; and (iii) reducing the embodied carbon intensity from compact urban planning policy, such as the urban agglomeration policies in GBA. The goal of this paper was to shed a light on reducing carbon emissions from the perspective of the entire lifecycle and promote the development of net zero carbon buildings in China and Asia-Pacific.
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| 6. |
- Weinstein, John N., et al.
(författare)
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The cancer genome atlas pan-cancer analysis project
- 2013
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Ingår i: Nature Genetics. - : Springer Science and Business Media LLC. - 1546-1718 .- 1061-4036. ; 45:10, s. 1113-1120
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Tidskriftsartikel (refereegranskat)abstract
- The Cancer Genome Atlas (TCGA) Research Network has profiled and analyzed large numbers of human tumors to discover molecular aberrations at the DNA, RNA, protein and epigenetic levels. The resulting rich data provide a major opportunity to develop an integrated picture of commonalities, differences and emergent themes across tumor lineages. The Pan-Cancer initiative compares the first 12 tumor types profiled by TCGA. Analysis of the molecular aberrations and their functional roles across tumor types will teach us how to extend therapies effective in one cancer type to others with a similar genomic profile. © 2013 Nature America, Inc. All rights reserved.
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| 7. |
- Dong, Tianyun, et al.
(författare)
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Effects of simplifying train bogies on surrounding flow and aerodynamic forces
- 2019
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Ingår i: Journal of Wind Engineering and Industrial Aerodynamics. - : Elsevier BV. - 0167-6105. ; 191, s. 170-182
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Tidskriftsartikel (refereegranskat)abstract
- © 2019 Elsevier Ltd In this study, the numerical solution of a high-speed train with several simplified bogies is investigated. The time-averaged flow field around the train, the surface pressure, and the aerodynamic forces on the train are discussed. The results reveal that a simpler bogie structure can achieve a higher underbody flow velocity and change fluctuations beneath the train owing to the resulting turbulence level. The simplification of bogies has a smaller effect on the side slipstream velocity and pressure compare to which in underbody, and at 3 m away from the centre of the track, the simplified bogie with wheels and a simple side frame used in this study obtains similar results to cases wherein more complex bogies are used. The surface pressure under the train is affected by bogie simplification, especially in the bogie cabin end area, resulting in aerodynamic drag and lift variations. If underbody flow or aerodynamic drag and lift forces are the focus of study, then the geometry of the centre region of the bogie, i.e. its main structures features, should be maintained in simplified models.
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| 8. |
- Dong, Tianyun, 1990, et al.
(författare)
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Numerical investigation of a high-speed train underbody flows: Studying flow structures through large-eddy simulation and assessment of steady and unsteady Reynolds-averaged Navier-Stokes and improved delayed detached eddy simulation performance
- 2022
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Ingår i: Physics of Fluids. - : AIP Publishing. - 1070-6631 .- 1089-7666. ; 34:1
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Tidskriftsartikel (refereegranskat)abstract
- The underbody flow of a truncated, 1:10 scaled, CRH380A model is investigated at Re = 2.78 × 105 in this paper. The large-eddy simulation (LES) is used to study the main features of the development of the underbody flow under the snowplow, in the bogie/cavity region and after the cavity (equip-cabin region). A grid independence study and a validation against experimental data have been done prior to the investigation. The snowplow region is dominated by a pair of separated counter-rotating vortices, which further affects the downstream flow. A strong shear layer is observed in the cavity region, and the turbulent flow is intensively triggered by the shear instability and the complex bogie components within the cavity region. The equip-cabin region allows the turbulent flow to develop without any disturbance, decreasing the turbulence intensity. Moreover, the steady and unsteady Reynolds-averaged Navier-Stokes (RANS, URANS) model and the improved delayed detached eddy simulation (IDDES) are used to compute the same flow, and to compare the results to LES. The solution differences, in terms of aerodynamic forces and the underbody flow state, are analyzed. Specifically, the minimum velocity discrepancy, at line2, between RANS (URANS) and LES is 14.4%, while IDDES is 3.6%. The solution accuracy vs the computational cost is also reported.
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| 9. |
- Dong, Tianyun, et al.
(författare)
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The effect of ground clearance on the aerodynamics of a generic high-speed train
- 2020
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Ingår i: Journal of Fluids and Structures. - : Elsevier BV. - 1095-8622 .- 0889-9746. ; 95
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Tidskriftsartikel (refereegranskat)abstract
- The influence of ground clearance on the flow around a simplified high-speed train is investigated in this paper. Four clearance heights are studied using IDDES. After a grid independence study, the results of the simulations are validated against experimental data present in the literature. It is found that the drag decreases when reducing the clearance gap from the baseline height to a possibly critical height, while drag remains constant when the clearance is lower than this critical height. The negative lift (downforce) increases with the decreasing of the clearance gap. The flow is particularly influenced by the gap height at the underbody and wake regions, where a lower underbody velocity and a higher wake velocity are observed with lower clearance down to case h2. Therefore, the different topologies of the wake are presented and described. Particular attention is paid to the description of the wake flow and to the position and the formation of the flow mixing region. Specifically, with decreasing clearance, the mix of the tail downwash and underbody flows happen earlier, and the core of the counter-rotating vortices in the wake tends to develop with an increasing height trend. Overall, aerodynamic performance and flow structure descriptions show positive and negative effects when decreasing gap clearances, which should be taken into account for new design strategies.
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| 10. |
- Dong, Tianyun, et al.
(författare)
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The effect of reducing the underbody clearance on the aerodynamics of a high-speed train
- 2020
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Ingår i: Journal of Wind Engineering and Industrial Aerodynamics. - : Elsevier BV. - 0167-6105. ; 204
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Tidskriftsartikel (refereegranskat)abstract
- The effect of lowering the ground clearance, on train aerodynamics is investigated using IDDES. The high speed train geometry with its full underbody complexity is used in the investigation. The clearance is reduced by installing extra panels on the track. The numerical results are verified and validated by a grid independence study and experimental data. This work shows that, when lowered the clearance, the underbody velocity at the head car decreases, while the underbody velocity at the middle and tail car increases. The reduced clearance barely affects the time-averaged slipstream at 3 m away from the center of track. However, at a closer distance to the train body, the difference in velocity is observed to reach up to 50% between the two clearance configurations and the clearance has an opposite effect on the trackside and platform slipstream. Based on the analysis of ensemble-averaged slipstream, lowering the underbody clearance, the characteristic air speed at the trackside and platform height increases by 2.0% and 6.7%, respectively. The total drag is almost unaffected by the clearance, but the changed distribution of the drag indicates a larger drag depends on the bogie structure if longer grouped train is used. The total lift decreases 25.9% after the clearance is reduced. Specifically, 4.1% of the negative lift is increased at the head car, while the positive lift decreases 92.7% and 1.8% for the middle and tail car, respectively. Overall it is shown that reducing the underbody clearance barely affect the slipstream at standard positions, but affects more aerodynamic loads of the train.
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