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- Appelberg, Jonas, 1964-, et al.
(författare)
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Lung aeration during sleep in patients with obstructive sleep apnoea
- 2010
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Ingår i: Clinical Physiology and Functional Imaging. - Oxford : Blackwell Publishing. - 1475-0961 .- 1475-097X. ; 30:4, s. 301-307
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Tidskriftsartikel (refereegranskat)abstract
- P>Background: Previous studies have indicated that patients with obstructive sleep apnoea (OSA) have altered ventilation and lung volumes awake and the results suggest that this may be a determinant of severity of desaturations during sleep. However, little is known about regional lung aeration during sleep in patients with OSA. Methods: Twelve patients with OSA were included in the study. Computed tomography was used to study regional lung aeration during wakefulness and sleep. Lung aeration was calculated in ml gas/g lung tissue in four different regions of interest (ROI1-4), along the border of the lung from ventral to dorsal. Results: Lung aeration in the dorsal (dependent) lung region (ROI4) was lower during sleep compared to wakefulness 0 center dot 78 +/- 0 center dot 19 versus 0 center dot 88 +/- 0 center dot 19 (mean +/- SD) ml gas/g lung tissue (P = 0 center dot 005). Associations were found between awake expiratory reserve volume and change in lung aeration from wakefulness to sleep in ROI4 (r = -0 center dot 69; P = 0 center dot 012). In addition, the change in lung aeration in the dorsal region correlated to sleep time (r = 0 center dot 69; P = 0 center dot 014) but not to time in supine position. The difference in lung aeration between inspiration and expiration (i.e. ventilation), was larger in the ventral lung region when expressed as ml gas per g lung tissue. In two patients it was noted that, during on-going obstructive apnoea, lung aeration tended to be increased rather than decreased. Conclusions: Aeration in the dorsal lung region is reduced during sleep in patients with OSA. The decrease is related to lung volume awake and to sleep time.
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- Enoksson, Fredrik, 1977-, et al.
(författare)
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Using a Hematology Curriculum in a Web Portfolio Environment
- 2011
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Ingår i: Knowledge Management & E-Learning: An International Journal. - 2073-7904. ; 3:1, s. 84-97
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Tidskriftsartikel (refereegranskat)abstract
- In 2005 the European Hematology Association developed theEuropean Hematology Curriculum. This was distributed as a printed bookletand the intention was that junior hematologist could use it for personalcompetence development. In the EU-funded project H-net this Curriculum hasbeen adapted into the a web environment by using RDF and placed inside aweb portfolio system. How this is done is further described in this article.Furthermore, the possibilities of reusing the curriculum in ways that was notinitially intended is described, such as describing Learning Resources inside theweb-portfolio system with how they relate to different parts of the curriculum.That way a search for learning resources inside the portfolio by using thecurriculum is enabled. And, since the medical field of hematology is closelyrelated to other medical fields the design of the web-version of the curriculumwas done in a way that builds for possible combination with any othercurriculum in another medical field.
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| 4. |
- Jensen, Poul Erik H., et al.
(författare)
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Detection and kinetics of persistent neutralizing anti-interferon-beta antibodies in patients with multiple sclerosis : Results from the ABIRISK prospective cohort study
- 2019
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Ingår i: Journal of Neuroimmunology. - : Elsevier. - 0165-5728 .- 1872-8421. ; 326, s. 19-27
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Tidskriftsartikel (refereegranskat)abstract
- Two validated assays, a bridging ELISA and a luciferase-based bioassay, were compared for detection of anti-drug antibodies (ADA) against interferon-beta (IFN-β) in patients with multiple sclerosis. Serum samples were tested from patients enrolled in a prospective study of 18 months. In contrast to the ELISA, when IFN-β-specific rabbit polyclonal and human monoclonal antibodies were tested, the bioassay was the more sensitive to detect IFN-β ADA in patients' sera. For clinical samples, selection of method of ELISA should be evaluated prior to the use of a multi-tiered approach. A titer threshold value is reported that may be used as a predictor for persistently positive neutralizing ADA.
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| 5. |
- Lindberg, Aleksandra
(författare)
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Efficiency and Selectivity in the Chlorate Process
- 2021
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This licentiate thesis presents experimental studies concerning two parts of the electrochemical cell in the chlorate process: a cathode and an anode.Newly synthesized MnOx electrodes were investigated for the cathodic reaction, hydrogen evolution reaction (HER) in the chlorate process. In industry addition of toxic and carcinogenic chromium (VI) as sodium dichromate provides high efficiency. Here undesirable addition of sodium dichromate was avoided while high cathodic efficiency was achieved. Cathodic efficiency and selectivity towards HER, achieved by the MnOx electrodes annealed at different temperatures, were measured by means of mass spectrometry (MS). The second study investigated oxygen evolution in the chlorate process, which is an anodic side reaction. The evolution of oxygen decreases anodic efficiency and also presents a safety risk due to occurrence of HER in the undivided cell. We followed the amount of produced oxygen by two types of the electrode TiRu, similar to that industrially used, and synthesized TiRuSnSb, by means of MS. The produced oxygen amount was compared to the amount produced by Pt. To our best knowledge, this was the first study that successfully disentangles three different sources of oxygen with good time resolution. Oxygen is produced by homogenous hypochlorite decomposition, heterogeneously by different catalysts present in the electrolyte solution and anodically during the electrolysis i.e. electrochemically. Different electrode materials catalyzed hypochlorite decomposition differently and led to a different volume of oxygen produced.
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- Lindberg, Eva-Rut, 1958-
(författare)
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Byggprojektering för personer med funktionsnedsättningen elöverkänslighet : ett arbete i spänningsfältet mellan tro och vetande
- 2011
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Elöverkänslighet är ett officiellt funktionshinder. Enligt Socialstyrelsens Miljöhälsorapport 2009 uppger 3,2 procent av befolkningen i åldrarna mellan 18 och 80 år att de är känsliga/överkänsliga eller allergiska mot elektriska och magnetiska fält, vilket motsvarar drygt 200 000 personer. 0,4 procent uppger att de har svåra besvär. Detta motsvarar knappt 30 000 personer som får svåra sjukdomsliknande symptom som exempelvis yrsel, illamående, huvudvärk, sömnproblem och hudbesvär när de vistas i elintensiva miljöer som större delen av befolkningen inte besväras av. Avhandlingen består dels av teoretiska studier över radiofrekvent strålning och hur denna kan avskärmas, dels av en enkätundersökning för att undersöka hur landets kommuner, försäkringskassekontor och tre landsting bemöter dessa personer. Genom den arkitektorienterade forskningsmetoden ”research by design” har ett förslag på ett elfritt typhus, som även kan avskärma de högre frekvenserna från den radiofrekventa strålningen, utformats men ännu inte byggts. Utifrån enkätundersökningen framgår att personer med funktionsnedsättningen elöverkänslighet ytterst sällan får hjälp med den tillgänglighetsanpassning i bostaden de enligt svensk lag har rätt till. Avhandlingen visar på vad som byggtekniskt kan utföras för att förbättra boendemiljön för personer med funktionsnedsättningen elöverkänslighet.
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| 9. |
- Ofverholm, Ingegerd, et al.
(författare)
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Comprehensive Genomic Profiling Alters Clinical Diagnoses in a Significant Fraction of Tumors Suspicious of Sarcoma
- 2024
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Ingår i: Clinical Cancer Research. - : American Association for Cancer Research (AACR). - 1078-0432 .- 1557-3265. ; 30:12, s. 2647-2658
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: Tumor classification is a key component in personalized cancer care. For soft-tissue and bone tumors, this classification is currently based primarily on morphology assessment and IHC staining. However, these standard-of-care methods can pose challenges for pathologists. We therefore assessed how whole-genome and whole-transcriptome sequencing (WGTS) impacted tumor classification and clinical management when interpreted together with histomorphology.Experimental Design: We prospectively evaluated WGTS in routine diagnostics of 200 soft-tissue and bone tumors suspicious for malignancy, including DNA and RNA isolation from the tumor, and DNA isolation from a peripheral blood sample or any non-tumor tissue.Results: On the basis of specific genomic alterations or absence of presumed findings, WGTS resulted in reclassification of 7% (13/197) of the histopathologic diagnoses. Four cases were downgraded from low-grade sarcomas to benign lesions, and two cases were reclassified as metastatic malignant melanomas. Fusion genes associated with specific tumor entities were found in 30 samples. For malignant soft-tissue and bone tumors, we identified treatment relevant variants in 15% of cases. Germline pathogenic variants associated with a hereditary cancer syndrome were found in 22 participants (11%).Conclusions: WGTS provides an important dimension of data that aids in the classification of soft-tissue and bone tumors, correcting a significant fraction of clinical diagnoses, and identifies molecular targets relevant for precision medicine. However, genetic findings need to be evaluated in their morphopathologic context, just as germline findings need to be evaluated in the context of patient phenotype and family history.
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| 10. |
- Orsmark-Pietras, Christina, et al.
(författare)
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Precision Diagnostics in Myeloid Malignancies : Development and Validation of a National Capture-Based Gene Panel
- 2024
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Ingår i: Genes Chromosomes and Cancer. - : Wiley. - 1045-2257 .- 1098-2264. ; 63:7
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Tidskriftsartikel (refereegranskat)abstract
- Gene panel sequencing has become a common diagnostic tool for detecting somatically acquired mutations in myeloid neoplasms. However, many panels have restricted content, provide insufficient sensitivity levels, or lack clinically validated workflows. We here describe the development and validation of the Genomic Medicine Sweden myeloid gene panel (GMS-MGP), a capture-based 191 gene panel including mandatory genes in contemporary guidelines as well as emerging candidates. The GMS-MGP displayed uniform coverage across all targets, including recognized difficult GC-rich areas. The validation of 117 previously described somatic variants showed a 100% concordance with a limit-of-detection of a 0.5% variant allele frequency (VAF), achieved by utilizing error correction and filtering against a panel-of-normals. A national interlaboratory comparison investigating 56 somatic variants demonstrated highly concordant results in both detection rate and reported VAFs. In addition, prospective analysis of 323 patients analyzed with the GMS-MGP as part of standard-of-care identified clinically significant genes as well as recurrent mutations in less well-studied genes. In conclusion, the GMS-MGP workflow supports sensitive detection of all clinically relevant genes, facilitates novel findings, and is, based on the capture-based design, easy to update once new guidelines become available. The GMS-MGP provides an important step toward nationally harmonized precision diagnostics of myeloid malignancies.
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