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Sökning: WFRF:(Lindner Anna) > Naturvetenskap

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1.
  • Menkveld, Albert J., et al. (författare)
  • Nonstandard Errors
  • 2024
  • Ingår i: JOURNAL OF FINANCE. - : Wiley-Blackwell. - 0022-1082 .- 1540-6261. ; 79:3, s. 2339-2390
  • Tidskriftsartikel (refereegranskat)abstract
    • In statistics, samples are drawn from a population in a data-generating process (DGP). Standard errors measure the uncertainty in estimates of population parameters. In science, evidence is generated to test hypotheses in an evidence-generating process (EGP). We claim that EGP variation across researchers adds uncertainty-nonstandard errors (NSEs). We study NSEs by letting 164 teams test the same hypotheses on the same data. NSEs turn out to be sizable, but smaller for more reproducible or higher rated research. Adding peer-review stages reduces NSEs. We further find that this type of uncertainty is underestimated by participants.
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2.
  • Nilsson, R. Henrik, 1976, et al. (författare)
  • Improving ITS sequence data for identification of plant pathogenic fungi
  • 2014
  • Ingår i: Fungal Diversity. - : Springer Science and Business Media LLC. - 1560-2745 .- 1878-9129. ; 67:1, s. 11-19
  • Tidskriftsartikel (refereegranskat)abstract
    • Plant pathogenic fungi are a large and diverse assemblage of eukaryotes with substantial impacts on natural ecosystems and human endeavours. These taxa often have complex and poorly understood life cycles, lack observable, discriminatory morphological characters, and may not be amenable to in vitro culturing. As a result, species identification is frequently difficult. Molecular (DNA sequence) data have emerged as crucial information for the taxonomic identification of plant pathogenic fungi, with the nuclear ribosomal internal transcribed spacer (ITS) region being the most popular marker. However, international nucleotide sequence databases are accumulating numerous sequences of compromised or low-resolution taxonomic annotations and substandard technical quality, making their use in the molecular identification of plant pathogenic fungi problematic. Here we report on a concerted effort to identify high-quality reference sequences for various plant pathogenic fungi and to re-annotate incorrectly or insufficiently annotated public ITS sequences from these fungal lineages. A third objective was to enrich the sequences with geographical and ecological metadata. The results – a total of 31,954 changes – are incorporated in and made available through the UNITE database for molecular identification of fungi (http://unite.ut.ee), including standalone FASTA files of sequence data for local BLAST searches, use in the next-generation sequencing analysis platforms QIIME and mothur, and related applications. The present initiative is just a beginning to cover the wide spectrum of plant pathogenic fungi, and we invite all researchers with pertinent expertise to join the annotation effort.
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3.
  • Gibaud, Thomas, et al. (författare)
  • New routes to food gels and glasses
  • 2012
  • Ingår i: Faraday Discussions. - : Royal Society of Chemistry (RSC). - 1364-5498 .- 1359-6640. ; 158, s. 267-284
  • Tidskriftsartikel (refereegranskat)abstract
    • We describe the possibility to create solid-like protein samples whose structural and mechanical properties can be varied and tailored over an extremely large range in a very controlled way through an arrested spinodal decomposition process. We use aqueous lysozyme solutions as a model globular protein system. A combination of video microscopy, small-angle neutron and X-ray scattering and reverse Monte Carlo modeling is used to characterize the structure of the bicontinuous network with two coexisting phases of a dilute protein solution and a glassy or arrested dense protein backbone at all relevant length scales. Rheological measurements are then used to determine the complex mechanical response of these protein gels as a function of protein concentration and quench temperature. While in particular the origin of the dependence of the mechanical properties on quench depth and concentration is not well understood currently, it seems ultimately connected to the particular bicontinuous structure of the arrested spinodal network created by the interplay between the early stage of a spinodal decomposition and the position of the glass line. We then generalize this behavior and discuss how this could open up new routes to prepare gel-like food systems with adjustable structural and mechanical properties. We present results from a first feasibility study where we use a depletion interaction caused by the addition of small non-adsorbing polymers to suspensions of casein micelles in order to create food gels with tunable structural and mechanical properties through an arrested spinodal decomposition process.
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4.
  • Kalinina, Iana, et al. (författare)
  • Pivoting of microtubules around the spindle pole accelerates kinetochore capture.
  • 2013
  • Ingår i: Nature Cell Biology. - : Springer Science and Business Media LLC. - 1465-7392 .- 1476-4679. ; 15:1, s. 82-7
  • Tidskriftsartikel (refereegranskat)abstract
    • During cell division, spindle microtubules attach to chromosomes through kinetochores, protein complexes on the chromosome. The central question is how microtubules find kinetochores. According to the pioneering idea termed search-and-capture, numerous microtubules grow from a centrosome in all directions and by chance capture kinetochores. The efficiency of search-and-capture can be improved by a bias in microtubule growth towards the kinetochores, by nucleation of microtubules at the kinetochores and at spindle microtubules, by kinetochore movement, or by a combination of these processes. Here we show in fission yeast that kinetochores are captured by microtubules pivoting around the spindle pole, instead of growing towards the kinetochores. This pivoting motion of microtubules is random and independent of ATP-driven motor activity. By introducing a theoretical model, we show that the measured random movement of microtubules and kinetochores is sufficient to explain the process of kinetochore capture. Our theory predicts that the speed of capture depends mainly on how fast microtubules pivot, which was confirmed experimentally by speeding up and slowing down microtubule pivoting. Thus, pivoting motion allows microtubules to explore space laterally, as they search for targets such as kinetochores.
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5.
  • Törnkvist, Anna, 1973- (författare)
  • Aspects of Porous Graphitic Carbon as Packing Material in Capillary Liquid Chromatography
  • 2003
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • In this thesis, porous graphitic carbon (PGC) has been used as packing material in packed capillary liquid chromatography. The unique chromatographic properties of PGC has been studied in some detail and applied to different analytical challenges using both electrospray ionization-mass spectrometry (ESI-MS) and ultra violet (UV) absorbance detection.The crucial importance of disengaging the conductive PGC chromatographic separation media from the high voltage mass spectrometric interface has been shown. In the absence of a grounded point between the column and ESI emitter, a current through the column was present, and changed retention behaviors for 3-O-methyl-DOPA and tyrosine were observed. An alteration of the chromatographic properties was also seen when PGC was chemically oxidized with permanganate, possibly due to an oxidation of the few surface groups present on the PGC material.The dynamic adsorption of the chiral selector lasalocid onto the PGC support resulted in a useful and stable chiral stationary phase. Extraordinary enantioselectivity was observed for 1-(1-naphthyl)ethylamine, and enantioseparation was also achieved for other amines, amino acids, acids and alcohols.Finally, a new strategy for separation of small biologically active compounds in plasma and brain tissue has been developed. With PGC as stationary phase it was possible to utilize a mobile phase of high content of organic modifier, without the addition of ion-pairing agents, and still selectively separate the analytes.
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